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Alternate Names / Synonyms: Murine IL-10, IL-10, IL10, IL 10, m-IL-10, rm-IL-10, recombinant murine IL-10, recombinant IL-10, IL, interluekin
Gene Symbol: IL10
Accession #: P18893
Gene ID: 16153
Source: E. coli
Appearance: Lyophilized protein
Physical Form Description: Lyophilized from 3 mM Tris, pH 8.0
Molecular Weight: 18.7 kDa
Purity by SDS-PAGE: ≥98%
Purity by HPLC: ≥98%
Endotoxin Level: <0.1 ng/μg
Biological Activity: The ED₅₀ determined by the dose-dependent co-stimulation （with murine IL-4） of MC/9 cells is <2 ng/ml, corresponding to a specific activity of > 5 x 10⁵ units/mg.
Reconstitution Instructions: Reconstitute in 3 mM Tris, pH 8.0 to a concentration of 0.1-1.0 µg/µl. The solution can then be diluted into other aqueous buffers ａnd stored at 4°C for 1 week or –20°C for future use.
Storage Temp.: -20°C
Shipping: Gel pack
Background Information: IL-10 is an immunosuppressive cytokine produced by a variety of mammalian cell types including macrophages, monocytes, T cells, B cells ａnd keratinocytes. IL-10 inhibits the expression of proinflammatory cytokines such as IL-1 ａnd TNF-ƒÑ. Like IL-4, IL-10 enhances humoral immune responses ａnd attenuates cell-mediated immune reactions. Human IL-10 active on murine cells, but murine IL-10 is inactive on human cells. Recombinant murine IL-10 is an 18.7 kDa protein of 161 amino acid residues.
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans.
