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Alternate Names / Synonyms: Human ApoE2, ApoE2, Apo-E2, Apo E2, h-ApoE2, rh-ApoE2, recombinant human ApoE2, recombinant ApoE2, Apo
Gene Symbol: ApoE
Accession #: NM_000041
Gene ID: 348
Source: E. coli
Appearance: Lyophilized protein
Physical Form Description: Sterile filtered ａnd lyophilized with no additives
Molecular Weight: 34.3 kDa
Purity by SDS-PAGE: ≥90%
Purity by HPLC: ≥90%
Endotoxin Level: <0.1 ng/μg
Biological Activity: Test in process
Reconstitution Instructions: Reconstitute in dH₂O to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers ａnd store at 4°C for 1 week or –20°C for future use.
Storage Temp.: -20°C
Shipping: Blue ice
Background Information: ApoE belongs to a group of proteins that bind reversibly with lipoprotein ａnd play an important role in lipid metabolism. In addition to facilitating solublization of lipids, these proteins help to maintain the structural integrity of lipoproteins, serve as ligands for lipoprotein receptors, ａnd regulate the activity of enzymes involved in lipid metabolism. Significant quantities of ApoE are produced in liver ａnd brain ａnd to some extent in almost every organ. ApoE is an important constituent of all plasma lipoproteins. It’s interaction with specific ApoE receptor enables uptake of chylomicron remnants by liver cells, which is an essential step during normal lipid metabolism. It also binds with the LDL receptor （apo B/E）. Defects in ApoE are a cause of hyperlipoproteinemia type III. ApoE exists in three major isoforms; E2, E3, ａnd E4, which differ from one another by a single amino-acid substitution. Compared with E3 ａnd E4, E2 exhibits the lowest receptor binding affinity. E2 allele carriers had significantly lower levels of total cholesterol, low-density lipoprotein cholesterol, ａnd non-high-density lipoprotein cholesterol, as well as increased ApoE levels. Recombinant human ApoE2 is a 34.3 kDa protein containing 300 amino acid residues.
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans.
