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DescriptionMeningeal cells surrounding the brain participate actively in the normal development of the central nervous system. For example, they play important roles in both stabilizing the extracellular matrix of the pial surface ａnd by organizing the radial glial scaffold ａnd the lamination of the cerebellar cortex [1]. Selective pharmacological destruction of the meningeal cells during a critical ontogenetic period leads to specific malformation of both the cerbella cortex ａnd dentate gyrus [1]. Grafts of meningeal cells, which derived form meninges overlying the cerebral cortex, in adult rat spinal cord lesion promote axonal regrowth [2]. In vitro study show that meningeal cell chemotactically orient the migration of immature neurons but not glial cells [3].MMC from ScienCell Research Laboratories are isolated from mouse leptomeningi. MMC are cryopreserved at passage one cultures ａnd delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. MMC are characterized by immunofluorescent method with antibodies to fibronectin ａnd negative to GFAP, alpha-smooth muscle actin ａnd Thy 1.1. MMC are negative for mycoplasma, bacteria, yeast ａnd fungi. MMC are guaranteed to further expand for 15 population doublings in the conditions specified by ScienCell Research Laboratories.
Recommended MediumIt is recommended to use Meningeal Cell Medium （MCM, Cat. No. 1401） for the culturing of MMC in vitro.
Product UseMMC are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
StorageTransfer directly ａnd immediately cells from dry ice to liquid nitrogen upon receiving ａnd keep the cells in liquid nitrogen until cell culture needed for experiments.
ShippingDry ice. |
