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DescriptionFibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular ａnd molecular studies. This is mainly because they are one of easiest types of cells to grow in culture, ａnd their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. There is good evidence that fibroblasts in different parts of the body are intrinsically different [1]. Fibroblasts within tissues are exposed to a dynamic mechnical environment, which influences the structure integrity of both healthy ａnd healing soft tissue. Fibroblasts secrete a nonrigid extracellular matrix that is rich in type I and/or type III collagen [2]. Besides, it has been reported that the growth of splenic fibroblasts has been increased in lymphoproliferatived diseases [3].MLF from ScienCell Research Laboratories are isolated from mouse lymph nodes. MLF are cryopreserved at primary culture ａnd delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. MLF are characterized by their spindle morphology ａnd immunofluorescent method with antibody to fibronectin. MLF are negative mycoplasma, bacteria, yeast ａnd fungi. MLF are guaranteed to further expand for 10 population doublings at the condition provided by ScienCell Research Laboratories.
Recommended MediumIt is recommended to use Fibroblast Medium （FM, Cat. No. 2301） for the culturing of MLF in vitro.
Product UseMLF are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
StorageDirectly ａnd immediately transfer cells from dry ice to liquid nitrogen upon receiving ａnd keep the cells in liquid nitrogen until cell culture needed for experiments.
ShippingDry ice. |
