Human Cell^exp Human Recombinant IL-28A

Product Name: Human Cell^exp Human Recombinant IL-28A

Alternate Name/Synonyms: Interleukin-28A, IL-28A, IFN-Lambda 2, Interferon-Lambda 2, Cytokine ZCYTO20, IL28A, IFNL2, ZCYTO20.

Gene Symbol: IFNL2

Accession #: Q8IZJ0

Gene ID: 282616

Source: Human 293 cell expressed

Appearance: Lyophilized

Physical Form Description: Lyophilized in 10 mM Tris, pH 7.4 ａnd 250 mM NaCl.

Molecular Weight: 24 kDa, monomer, non-glycosylated

Purity by SDS-PAGE: ≥95%

Purity by HPLC: N/A

Endotoxin Level: < 1.0 EU per 1 μg of protein

Biological Activity: ED₅₀ is typically 0.5 to 5 ng/mL. The specific activity was determined by the dose-dependent protection of the cytopathic effect on A549 cells （human lung adenocarcinoma epithelial cell line） that were challenged with encephalomyocarditis （EMC） virus.

Reconstitution Instructions: Reconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.

Storage Temp.: -80°C

Shipping: Gel pack

Background Information: IL-28A is distantly related to type I interferons ａnd the IL-10 family. Expression of IL-28A is induced by viral infection which interacts with a heterodimeric class II cytokine receptor that consists of interleukin 10 receptor, beta （IL10RB） ａnd interleukin 28 receptor, alpha. IL-28A exhibits common features with type I IFNs such as antiviral activity, antiproliferative activity ａnd in vivo antitumor activity. They act similarly to IFNs, but are less effective generally ａnd have activity in a more limited range of cell lines. IL-28A induces ELR （-） CXC chemokine mRNA in human peripheral blood mononuclear cells, in an IFN-gamma-independent manner. It is able to generate tolerogenic DCs, an activity that could thwart IFN-beta functions. IL-28A produced in response to viral infection, activates both monocytes ａnd macrophages producing a restricted panel of cytokines ａnd therefore is an important factor in activating innate immune responses at the site of viral infection.

Amino Acid Sequence: N/A

Handling: Centrifuge the vial prior to opening

USAGE: For Research Use Only! Not For Use in Humans.