Human Cell^exp Human Recombinant TNF-alpha

Product Name: Human Cell^exp Human Recombinant TNF-alpha

Purity by SDS-PAGE: ≥95%

Purity by HPLC: N/A

Endotoxin Level: < 1.0 EU per 1 μg of protein

Biological Activity: ED₅₀ is typically < 0.9 ng/mL. The specific activity was determined by the dose-dependent cytotoxity of the TNF alpha sensitive cell line L-929 in the presence of Actinomycin D.

Reconstitution Instructions: Reconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.

Storage Temp.: -80°C

Shipping: Gel pack

Background Information: Tumor Necrosis Factor-α （TNF-α） is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells ａnd certain other target cells. TNF is a cytokine involved in systemic inflammation ａnd is a member of a group of cytokines that all stimulate the acute phase reaction. TNF is mainly secreted by macrophages. TNF causes apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis ａnd viral replication, TNF is also involved in lipid metabolism, ａnd coagulation. TNF＇s primary role is in the regulation of immune cells. Dysregulation and, in particular, overproduction of TNF have been implicated in a variety of human diseases- autoimmune diseases, insulin resistance, ａnd cancer.

Amino Acid Sequence: N/A

Handling: Centrifuge the vial prior to opening

USAGE: For Research Use Only! Not For Use in Humans.

Alternate Name/Synonyms: TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Cachectin, DIF, TNFA, TNFSF2.

Gene Symbol: TNF

Accession #: P01375

Gene ID: 7124

Source: Human 293 cell expressed

Appearance: Lyophilized

Physical Form Description: Lyophilized in PBS.

Molecular Weight: 17 kDa, non-disulfide bonded homotrimer, glycosylated