Description The tissue of the central nervous system is made up of two classes of cells that may be broadly categorized as neurons ａnd glia. Neurons are anatomic, functional, ａnd trophic units of the brain [1]. Despite great variability in size ａnd shape, all neurons share common morphologic features, which are those of the key elements of a highly complex communication network. The neurons are the dynamically polarized cells that serve as the major signaling unit of the nervous system. The human brain is known to contain about 1 x 1011 neurons, each being able to contact at least 10,000 other neurons [2]. The hippocampal neurons play a special role in learning ａnd memory ａnd cultured hippocampal neurons are a useful model for studying neuronal phenomena such as differentiation, survival, process growth, ａnd synapto-genesis. HHN from ScienCell Research Laboratories are isolated from hippocampal tissue of the brain. HHN are cryopreserved at secondary cultures ａnd delivered frozen. Each vial contains >1 x 106 cells in 1 ml volume. HHN are characterized by immunofluorescent method with antibodies to neurafilament, MAP2, ａnd beta-tubulin III. HHN are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast ａnd fungi. HHN are guaranteed to further culture in the conditions provided by ScienCell Research Laboratories. Recommended Medium It is recommended to use neuronal medium （NM, Cat. No. 1521） for the culture of human neurons in vitro. Product Use HHN are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures. Storage Transfer cells directly ａnd immediately from dry ice to liquid nitrogen upon receiving ａnd keep the cells in liquid nitrogen until cell culture is needed for experiments.