产品展示
人肠成纤维细胞
点击次数：191发布时间：2013/4/24 15:56:42
更新日期：2022/8/19 17:45:54
所 在 地：美洲
产品型号：
优质供应
详细内容
人肠成纤维细胞
上海义森生物科技有限公司
Description
Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular ａnd molecular studies. This is mainly because they are one of easiest types of cells to grow in culture, ａnd their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. There is good evidence that fibroblasts in different parts of the body are intrinsically different [1]. Fibroblasts secrete a non-rigid extracellular matrix that is rich in type I and/or type III collagen [2]. They are responsible for much of the synthesis of extracellular matrix in connective tissues ａnd play major roles in wound healing. Many diseases are associated with fibroblasts, either because fibroblasts are implicated in their etiology or because of the fibrosis that accompanies damage to other cell types in tissues. For example, the development of bowl stenosis in Crohn’s disease patients is caused by extreme fibroblast proliferation ａnd extracellular matrix expansion [3].
HIF from ScienCell Research Laboratories are isolated from human intestinal tissue. HIF are cryopreserved at passage one ａnd delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HIF are characterized by their spindle morphology ａnd immunofluorescent method with antibody to fibronectin. HIF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast ａnd fungi. HIF are guaranteed to further expand for 15 population doublings at the condition provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Fibroblast Medium （FM, Cat. No. 2301） for the culturing of HIF in vitro.
Product Use
HIF are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Directly ａnd immediately transfer cells from dry ice to liquid nitrogen upon receiving ａnd keep the cells in liquid nitrogen until cell culture needed for experiments.
Shipping
Dry ice.
Reference
[1] Conrad, G. W., Hart, G. W., Chen, Y. （1977） Differences in vitro between fibroblast-like cells from cornea, heart, ａnd skin of embryonic chicks. J. Cell Sci. 26:119-137.
[2] Gabbiani, G., Rungger-Brandle, E., The fibroblast. In Tissue Repair ａnd Regeneration （L. E. Glynn, ed.）, pp 1-50. Handbook of Inflammation, Vol. 3. Amsterdam, Elsevier, 1981.
[3] Luna, J., Masamunt, MC., Rickmann, M., Mora, R., Espana, C., Delgado, S., Llach, J., Vaquero, E., Sans, M. （2011） Rocotrienols have potent antifibrogenic effects in human intestinal fibroblasts. Inflamm Bowel Dis. 17（3）:732-41
Description
Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular ａnd molecular studies. This is mainly because they are one of easiest types of cells to grow in culture, ａnd their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. There is good evidence that fibroblasts in different parts of the body are intrinsically different [1]. Fibroblasts secrete a non-rigid extracellular matrix that is rich in type I and/or type III collagen [2]. They are responsible for much of the synthesis of extracellular matrix in connective tissues ａnd play major roles in wound healing. Many diseases are associated with fibroblasts, either because fibroblasts are implicated in their etiology or because of the fibrosis that accompanies damage to other cell types in tissues. For example, the development of bowl stenosis in Crohn’s disease patients is caused by extreme fibroblast proliferation ａnd extracellular matrix expansion [3].
HIF from ScienCell Research Laboratories are isolated from human intestinal tissue. HIF are cryopreserved at passage one ａnd delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HIF are characterized by their spindle morphology ａnd immunofluorescent method with antibody to fibronectin. HIF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast ａnd fungi. HIF are guaranteed to further expand for 15 population doublings at the condition provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Fibroblast Medium （FM, Cat. No. 2301） for the culturing of HIF in vitro.
Product Use
HIF are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Directly ａnd immediately transfer cells from dry ice to liquid nitrogen upon receiving ａnd keep the cells in liquid nitrogen until cell culture needed for experiments.
Shipping
Dry ice.
Reference
[1] Conrad, G. W., Hart, G. W., Chen, Y. （1977） Differences in vitro between fibroblast-like cells from cornea, heart, ａnd skin of embryonic chicks. J. Cell Sci. 26:119-137.
[2] Gabbiani, G., Rungger-Brandle, E., The fibroblast. In Tissue Repair ａnd Regeneration （L. E. Glynn, ed.）, pp 1-50. Handbook of Inflammation, Vol. 3. Amsterdam, Elsevier, 1981.
[3] Luna, J., Masamunt, MC., Rickmann, M., Mora, R., Espana, C., Delgado, S., Llach, J., Vaquero, E., Sans, M. （2011） Rocotrienols have potent antifibrogenic effects in human intestinal fibroblasts. Inflamm Bowel Dis. 17（3）:732-41
Description
Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular ａnd molecular studies. This is mainly because they are one of easiest types of cells to grow in culture, ａnd their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. There is good evidence that fibroblasts in different parts of the body are intrinsically different [1]. Fibroblasts secrete a non-rigid extracellular matrix that is rich in type I and/or type III collagen [2]. They are responsible for much of the synthesis of extracellular matrix in connective tissues ａnd play major roles in wound healing. Many diseases are associated with fibroblasts, either because fibroblasts are implicated in their etiology or because of the fibrosis that accompanies damage to other cell types in tissues. For example, the development of bowl stenosis in Crohn’s disease patients is caused by extreme fibroblast proliferation ａnd extracellular matrix expansion [3].
HIF from ScienCell Research Laboratories are isolated from human intestinal tissue. HIF are cryopreserved at passage one ａnd delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HIF are characterized by their spindle morphology ａnd immunofluorescent method with antibody to fibronectin. HIF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast ａnd fungi. HIF are guaranteed to further expand for 15 population doublings at the condition provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Fibroblast Medium （FM, Cat. No. 2301） for the culturing of HIF in vitro.
Product Use
HIF are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Directly ａnd immediately transfer cells from dry ice to liquid nitrogen upon receiving ａnd keep the cells in liquid nitrogen until cell culture needed for experiments.
Shipping
Dry ice.
Reference
[1] Conrad, G. W., Hart, G. W., Chen, Y. （1977） Differences in vitro between fibroblast-like cells from cornea, heart, ａnd skin of embryonic chicks. J. Cell Sci. 26:119-137.
[2] Gabbiani, G., Rungger-Brandle, E., The fibroblast. In Tissue Repair ａnd Regeneration （L. E. Glynn, ed.）, pp 1-50. Handbook of Inflammation, Vol. 3. Amsterdam, Elsevier, 1981.
[3] Luna, J., Masamunt, MC., Rickmann, M., Mora, R., Espana, C., Delgado, S., Llach, J., Vaquero, E., Sans, M. （2011） Rocotrienols have potent antifibrogenic effects in human intestinal fibroblasts. Inflamm Bowel Dis. 17（3）:732-41
