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人甲状腺成纤维细胞
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更新日期：2022/8/19 17:45:54
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人甲状腺成纤维细胞
上海义森生物科技有限公司
DescriptionFibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular ａnd molecular studies. This is mainly because they are one of easiest types of cells to grow in culture, ａnd their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. There is good evidence that fibroblasts in different parts of the body are intrinsically different [1]. Fibroblasts secrete a non-rigid extracellular matrix that is rich in type I and/or type III collagen [2]. They are responsible for much of the synthesis of extracellular matrix in connective tissues ａnd play major roles in wound healing. Many diseases are associated with fibroblasts, either because fibroblasts are implicated in their etiology or because of the fibrosis that accompanies damage to other cell types in tissues. Because the thyroid is easily susceptible to inflammatory autoimmune diseases that can result in tissue damage, thyroid fibroblasts may play an important role in regulating thyroid function ａnd reaction to disease [3]. HThF from ScienCell Research Laboratories are isolated from human thyroid tissue. HThF are cryopreserved at passage one ａnd delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HThF are characterized by their spindle morphology ａnd immunofluorescent method with antibody to fibronectin. HThF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast ａnd fungi. HThF are guaranteed to further expand for 15 population doublings at the condition provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Fibroblast Medium （FM, Cat. No. 2301） for the culturing of HThF in vitro.
Product Use
HThF are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Directly ａnd immediately transfer cells from dry ice to liquid nitrogen upon receiving ａnd keep the cells in liquid nitrogen until cell culture needed for experiments.
Shipping
Dry ice.
Reference
[1] Conrad, G. W., Hart, G. W., Chen, Y. （1977） Differences in vitro between fibroblast-like cells from cornea, heart, ａnd skin of embryonic chicks. J. Cell Sci. 26:119-137.
[2] Gabbiani, G., Rungger-Brandle, E., The fibroblast. In Tissue Repair ａnd Regeneration （L. E. Glynn, ed.）, pp 1-50. Handbook of Inflammation, Vol. 3. Amsterdam, Elsevier, 1981.
[3] Smith, et al. （1997） Human Thyroid Fibroblasts Exhibit a Distinctive Phenotype in Culture: Characteristic Ganglioside Profile ａnd Functional CD40 Expression. Endocrinology. 138 （12）: 5576-88
DescriptionFibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular ａnd molecular studies. This is mainly because they are one of easiest types of cells to grow in culture, ａnd their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. There is good evidence that fibroblasts in different parts of the body are intrinsically different [1]. Fibroblasts secrete a non-rigid extracellular matrix that is rich in type I and/or type III collagen [2]. They are responsible for much of the synthesis of extracellular matrix in connective tissues ａnd play major roles in wound healing. Many diseases are associated with fibroblasts, either because fibroblasts are implicated in their etiology or because of the fibrosis that accompanies damage to other cell types in tissues. Because the thyroid is easily susceptible to inflammatory autoimmune diseases that can result in tissue damage, thyroid fibroblasts may play an important role in regulating thyroid function ａnd reaction to disease [3]. HThF from ScienCell Research Laboratories are isolated from human thyroid tissue. HThF are cryopreserved at passage one ａnd delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HThF are characterized by their spindle morphology ａnd immunofluorescent method with antibody to fibronectin. HThF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast ａnd fungi. HThF are guaranteed to further expand for 15 population doublings at the condition provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Fibroblast Medium （FM, Cat. No. 2301） for the culturing of HThF in vitro.
Product Use
HThF are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Directly ａnd immediately transfer cells from dry ice to liquid nitrogen upon receiving ａnd keep the cells in liquid nitrogen until cell culture needed for experiments.
Shipping
Dry ice.
Reference
[1] Conrad, G. W., Hart, G. W., Chen, Y. （1977） Differences in vitro between fibroblast-like cells from cornea, heart, ａnd skin of embryonic chicks. J. Cell Sci. 26:119-137.
[2] Gabbiani, G., Rungger-Brandle, E., The fibroblast. In Tissue Repair ａnd Regeneration （L. E. Glynn, ed.）, pp 1-50. Handbook of Inflammation, Vol. 3. Amsterdam, Elsevier, 1981.
[3] Smith, et al. （1997） Human Thyroid Fibroblasts Exhibit a Distinctive Phenotype in Culture: Characteristic Ganglioside Profile ａnd Functional CD40 Expression. Endocrinology. 138 （12）: 5576-88
Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular ａnd molecular studies. This is mainly because they are one of easiest types of cells to grow in culture, ａnd their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. There is good evidence that fibroblasts in different parts of the body are intrinsically different [1]. Fibroblasts secrete a non-rigid extracellular matrix that is rich in type I and/or type III collagen [2]. They are responsible for much of the synthesis of extracellular matrix in connective tissues ａnd play major roles in wound healing. Many diseases are associated with fibroblasts, either because fibroblasts are implicated in their etiology or because of the fibrosis that accompanies damage to other cell types in tissues. Because the thyroid is easily susceptible to inflammatory autoimmune diseases that can result in tissue damage, thyroid fibroblasts may play an important role in regulating thyroid function ａnd reaction to disease [3]. HThF from ScienCell Research Laboratories are isolated from human thyroid tissue. HThF are cryopreserved at passage one ａnd delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HThF are characterized by their spindle morphology ａnd immunofluorescent method with antibody to fibronectin. HThF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast ａnd fungi. HThF are guaranteed to further expand for 15 population doublings at the condition provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Fibroblast Medium （FM, Cat. No. 2301） for the culturing of HThF in vitro.
Product Use
HThF are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Directly ａnd immediately transfer cells from dry ice to liquid nitrogen upon receiving ａnd keep the cells in liquid nitrogen until cell culture needed for experiments.
Shipping
Dry ice.
Reference
[1] Conrad, G. W., Hart, G. W., Chen, Y. （1977） Differences in vitro between fibroblast-like cells from cornea, heart, ａnd skin of embryonic chicks. J. Cell Sci. 26:119-137.
[2] Gabbiani, G., Rungger-Brandle, E., The fibroblast. In Tissue Repair ａnd Regeneration （L. E. Glynn, ed.）, pp 1-50. Handbook of Inflammation, Vol. 3. Amsterdam, Elsevier, 1981.
[3] Smith, et al. （1997） Human Thyroid Fibroblasts Exhibit a Distinctive Phenotype in Culture: Characteristic Ganglioside Profile ａnd Functional CD40 Expression. Endocrinology. 138 （12）: 5576-88
