产品展示
人股骨成骨细胞
点击次数：479发布时间：2013/4/24 17:57:55
更新日期：2022/8/19 17:45:54
所 在 地：美洲
产品型号：
优质供应
详细内容
人股骨成骨细胞
上海义森生物科技有限公司
Description
Bone is a dynamic tissue, being continuously remodeled by the coordinated actions of osteoclasts ａnd osteoblast lineage. Osteoblasts, the bone-forming cells, are derived originally from pluripotent mesenchymal stem cells. They synthesize ａnd secrete organic extracellular matrix, osteoid, which is composed primarily of type I collagen. Osteoid is calcified by osteoblasts ａnd during this process the cells become encased in lacunae within the calcified material ａnd become osteocytes. Osteoblasts express protease-activated receptor-1 ａnd vescular endothelial cell growth factor [1]. Studies show that Leukemia inhibitory factor can bind to the osteoblast cell surface ａnd induce bone formation both in vitro ａnd in vivo [2]. The balance between osteoblast recruitment, proliferation, differentiation ａnd apoptosis in sutures between cranial bones is essential for calvarial bone formation [3].
HO-f from ScienCell Research Laboratories are isolated from human femur. HO-f are cryopreserved at primary cultures ａnd delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HO-f are characterized by the cytochemically detection of AP ａnd mineral deposition. HO-f are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast ａnd fungi. HO-f are guaranteed for 15 population doublings at the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Osteoblast Medium （OsM, Cat. No. 4601） for the culturing of HO-f in vitro.
Product Use
HO-f are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Directly ａnd immediately transfer cells from dry ice to liquid nitrogen upon receiving ａnd keep the cells in liquid nitrogen until cell culture needed for experiments.
Shipping
Dry ice.
Reference
[1] Steinbrech, D. S., Mehrara, B. J., Saadeh, P. B., Greenwald, J.A., Spector, J. A., Gittes, G. K. ａnd Longaker, M. T. （2000） VEGF expression in an osteoblast-like cell line is regulated by a hypoxia response mechanism. Am. J. Physiol. Cell Physiol. 278: C853-C860.
[2] Dazai, S., Akita, S., Hirano, A., Rashid, M. A., Naito, S., Akino, K., Fujii, T. （2000） Leukemia inhibitory factor enhances bone formation in calvarial bone defect. J. Craniofac. Surg. 11（6）:513-20.
[3] Marie, P. J., Debiais, F., Hay, E. （2002） Regulation of human cranial osteoblast phenotype by FGF-2, FGFR-2 ａnd BMP-2 signaling. Histol. Histopathol.17（3）:877-85.
Description
Bone is a dynamic tissue, being continuously remodeled by the coordinated actions of osteoclasts ａnd osteoblast lineage. Osteoblasts, the bone-forming cells, are derived originally from pluripotent mesenchymal stem cells. They synthesize ａnd secrete organic extracellular matrix, osteoid, which is composed primarily of type I collagen. Osteoid is calcified by osteoblasts ａnd during this process the cells become encased in lacunae within the calcified material ａnd become osteocytes. Osteoblasts express protease-activated receptor-1 ａnd vescular endothelial cell growth factor [1]. Studies show that Leukemia inhibitory factor can bind to the osteoblast cell surface ａnd induce bone formation both in vitro ａnd in vivo [2]. The balance between osteoblast recruitment, proliferation, differentiation ａnd apoptosis in sutures between cranial bones is essential for calvarial bone formation [3].
HO-f from ScienCell Research Laboratories are isolated from human femur. HO-f are cryopreserved at primary cultures ａnd delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HO-f are characterized by the cytochemically detection of AP ａnd mineral deposition. HO-f are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast ａnd fungi. HO-f are guaranteed for 15 population doublings at the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Osteoblast Medium （OsM, Cat. No. 4601） for the culturing of HO-f in vitro.
Product Use
HO-f are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Directly ａnd immediately transfer cells from dry ice to liquid nitrogen upon receiving ａnd keep the cells in liquid nitrogen until cell culture needed for experiments.
Shipping
Dry ice.
Reference
[1] Steinbrech, D. S., Mehrara, B. J., Saadeh, P. B., Greenwald, J.A., Spector, J. A., Gittes, G. K. ａnd Longaker, M. T. （2000） VEGF expression in an osteoblast-like cell line is regulated by a hypoxia response mechanism. Am. J. Physiol. Cell Physiol. 278: C853-C860.
[2] Dazai, S., Akita, S., Hirano, A., Rashid, M. A., Naito, S., Akino, K., Fujii, T. （2000） Leukemia inhibitory factor enhances bone formation in calvarial bone defect. J. Craniofac. Surg. 11（6）:513-20.
[3] Marie, P. J., Debiais, F., Hay, E. （2002） Regulation of human cranial osteoblast phenotype by FGF-2, FGFR-2 ａnd BMP-2 signaling. Histol. Histopathol.17（3）:877-85.
Description
Bone is a dynamic tissue, being continuously remodeled by the coordinated actions of osteoclasts ａnd osteoblast lineage. Osteoblasts, the bone-forming cells, are derived originally from pluripotent mesenchymal stem cells. They synthesize ａnd secrete organic extracellular matrix, osteoid, which is composed primarily of type I collagen. Osteoid is calcified by osteoblasts ａnd during this process the cells become encased in lacunae within the calcified material ａnd become osteocytes. Osteoblasts express protease-activated receptor-1 ａnd vescular endothelial cell growth factor [1]. Studies show that Leukemia inhibitory factor can bind to the osteoblast cell surface ａnd induce bone formation both in vitro ａnd in vivo [2]. The balance between osteoblast recruitment, proliferation, differentiation ａnd apoptosis in sutures between cranial bones is essential for calvarial bone formation [3].
HO-f from ScienCell Research Laboratories are isolated from human femur. HO-f are cryopreserved at primary cultures ａnd delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HO-f are characterized by the cytochemically detection of AP ａnd mineral deposition. HO-f are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast ａnd fungi. HO-f are guaranteed for 15 population doublings at the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Osteoblast Medium （OsM, Cat. No. 4601） for the culturing of HO-f in vitro.
Product Use
HO-f are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Directly ａnd immediately transfer cells from dry ice to liquid nitrogen upon receiving ａnd keep the cells in liquid nitrogen until cell culture needed for experiments.
Shipping
Dry ice.
Reference
[1] Steinbrech, D. S., Mehrara, B. J., Saadeh, P. B., Greenwald, J.A., Spector, J. A., Gittes, G. K. ａnd Longaker, M. T. （2000） VEGF expression in an osteoblast-like cell line is regulated by a hypoxia response mechanism. Am. J. Physiol. Cell Physiol. 278: C853-C860.
[2] Dazai, S., Akita, S., Hirano, A., Rashid, M. A., Naito, S., Akino, K., Fujii, T. （2000） Leukemia inhibitory factor enhances bone formation in calvarial bone defect. J. Craniofac. Surg. 11（6）:513-20.
[3] Marie, P. J., Debiais, F., Hay, E. （2002） Regulation of human cranial osteoblast phenotype by FGF-2, FGFR-2 ａnd BMP-2 signaling. Histol. Histopathol.17（3）:877-85.
