Description

Degeneration of the intervertebral disk is a major pathological process implicated in low back pain. The causes of low back pain have been suggested to involve genetic, environmental, ａnd psychosocial factors [1, 2]. The anulus fibrosus makes up peripheral portion of disk structure, composed of fibrocartilage ａnd type I ａnd II collagen. Cells of the intervertebral disc are influenced by both biophysical ａnd mechanical factors in their local environment. The anulus fibrosus cells can be stimulated by interleukin 1 beta to produce factors implicated in local degradative ａnd inflammatory processes [3]. Although mechanical stress is an important modulator of the degeneration, the underlying molecular mechanism remains unclear. The human annulus firosus cell culture provides an in vitro model for the study of cellular ａnd molecular events involved in disc degeneration, tissue engineering ａnd cell therapy for spine disc disorders.

HAFC from ScienCell Research Laboratories are isolated from annulus fibrosus of human invertibral disc. HAFC are cryopreserved at primary or passage one culture ａnd delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HAFC are characterized by immunofluorescent method with antibodies to fibronectin ａnd vimentin. HAFC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast ａnd fungi. HAFC are guaranteed to further expand for 15 population doublings in the condition provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use nucleus pulposus cell medium （NPCM, Cat. No. 4801） for the culturing of HAFC in vitro.

Product Use

HAFC are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage

Directly ａnd immediately transfer cells from dry ice to liquid nitrogen upon receiving ａnd keep the cells in liquid nitrogen until cell culture needed for experiments.