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人脾脏成纤维细胞
上海义森生物科技有限公司
Description
Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular ａnd molecular studies. This is mainly because they are one of easiest types of cells to grow in culture, ａnd their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. Fibroblasts within tissues are exposed to a dynamic mechnical environment, which influences the structure integrity of both healthy ａnd healing soft tissue. They secrete a non-rigid extracellular matrix that is rich in type I and/or type III collagen [1]. There is good evidence that fibroblasts in different parts of the body are intrinsically different ａnd display different regulatory functions on surrounding cells [2]. For example, splenic fibroblasts have been shown to regulate the development of NK cells from blood progenitors through an expression of bioactive IL-15. [3]
HSF from ScienCell Research Laboratories are isolated from human spleen tissue. HSF are cryopreserved at primary culture ａnd delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HSF are characterized by their spindle morphology ａnd immunofluorescent method with antibody to fibronectin. HSF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast ａnd fungi. HSF are guaranteed to further expand for 15 population doublings at the condition provided by ScienCell Research Laboratories.
Recommended MediumIt is recommended to use Fibroblast Medium （FM, Cat. No. 2301） for the culturing of HSF in vitro.
Product Use
HSF are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Directly ａnd immediately transfer cells from dry ice to liquid nitrogen upon receiving ａnd keep the cells in liquid nitrogen until cell culture needed for experiments.
Shipping
Dry ice.
Reference
[1] Gabbiani, G., Rungger-Brandle, E., The fibroblast. In Tissue Repair ａnd Regeneration （L. E. Glynn, ed.）, pp 1-50. Handbook of Inflammation, Vol. 3. Amsterdam, Elsevier, 1981.
[2] Conrad, G. W., Hart, G. W., Chen, Y. （1977） Differences in vitro between fibroblast-like cells from cornea, heart, ａnd skin of embryonic chicks. J. Cell Sci. 26:119-137.
[3] Briard, D., Brouty-Boy?, D., Azzarone, B., Jasmin, C. （2002） Fibroblasts from human spleen regulate NK cell differentiation from blood CD34（+） progenitors via cell surface IL-15. J Immunol. 168（9）:4326-32
Description
Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular ａnd molecular studies. This is mainly because they are one of easiest types of cells to grow in culture, ａnd their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. Fibroblasts within tissues are exposed to a dynamic mechnical environment, which influences the structure integrity of both healthy ａnd healing soft tissue. They secrete a non-rigid extracellular matrix that is rich in type I and/or type III collagen [1]. There is good evidence that fibroblasts in different parts of the body are intrinsically different ａnd display different regulatory functions on surrounding cells [2]. For example, splenic fibroblasts have been shown to regulate the development of NK cells from blood progenitors through an expression of bioactive IL-15. [3]
HSF from ScienCell Research Laboratories are isolated from human spleen tissue. HSF are cryopreserved at primary culture ａnd delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HSF are characterized by their spindle morphology ａnd immunofluorescent method with antibody to fibronectin. HSF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast ａnd fungi. HSF are guaranteed to further expand for 15 population doublings at the condition provided by ScienCell Research Laboratories.
Recommended MediumIt is recommended to use Fibroblast Medium （FM, Cat. No. 2301） for the culturing of HSF in vitro.
Product Use
HSF are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Directly ａnd immediately transfer cells from dry ice to liquid nitrogen upon receiving ａnd keep the cells in liquid nitrogen until cell culture needed for experiments.
Shipping
Dry ice.
Reference
[1] Gabbiani, G., Rungger-Brandle, E., The fibroblast. In Tissue Repair ａnd Regeneration （L. E. Glynn, ed.）, pp 1-50. Handbook of Inflammation, Vol. 3. Amsterdam, Elsevier, 1981.
[2] Conrad, G. W., Hart, G. W., Chen, Y. （1977） Differences in vitro between fibroblast-like cells from cornea, heart, ａnd skin of embryonic chicks. J. Cell Sci. 26:119-137.
[3] Briard, D., Brouty-Boy?, D., Azzarone, B., Jasmin, C. （2002） Fibroblasts from human spleen regulate NK cell differentiation from blood CD34（+） progenitors via cell surface IL-15. J Immunol. 168（9）:4326-32
Description
Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular ａnd molecular studies. This is mainly because they are one of easiest types of cells to grow in culture, ａnd their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. Fibroblasts within tissues are exposed to a dynamic mechnical environment, which influences the structure integrity of both healthy ａnd healing soft tissue. They secrete a non-rigid extracellular matrix that is rich in type I and/or type III collagen [1]. There is good evidence that fibroblasts in different parts of the body are intrinsically different ａnd display different regulatory functions on surrounding cells [2]. For example, splenic fibroblasts have been shown to regulate the development of NK cells from blood progenitors through an expression of bioactive IL-15. [3]
HSF from ScienCell Research Laboratories are isolated from human spleen tissue. HSF are cryopreserved at primary culture ａnd delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HSF are characterized by their spindle morphology ａnd immunofluorescent method with antibody to fibronectin. HSF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast ａnd fungi. HSF are guaranteed to further expand for 15 population doublings at the condition provided by ScienCell Research Laboratories.
Recommended MediumIt is recommended to use Fibroblast Medium （FM, Cat. No. 2301） for the culturing of HSF in vitro.
Product Use
HSF are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Directly ａnd immediately transfer cells from dry ice to liquid nitrogen upon receiving ａnd keep the cells in liquid nitrogen until cell culture needed for experiments.
Shipping
Dry ice.
Reference
[1] Gabbiani, G., Rungger-Brandle, E., The fibroblast. In Tissue Repair ａnd Regeneration （L. E. Glynn, ed.）, pp 1-50. Handbook of Inflammation, Vol. 3. Amsterdam, Elsevier, 1981.
[2] Conrad, G. W., Hart, G. W., Chen, Y. （1977） Differences in vitro between fibroblast-like cells from cornea, heart, ａnd skin of embryonic chicks. J. Cell Sci. 26:119-137.
[3] Briard, D., Brouty-Boy?, D., Azzarone, B., Jasmin, C. （2002） Fibroblasts from human spleen regulate NK cell differentiation from blood CD34（+） progenitors via cell surface IL-15. J Immunol. 168（9）:4326-32
