产品展示
优质供应
详细内容
人冠状动脉内皮细胞
上海义森生物科技有限公司
Description
Endothelial cells constitute the natural interface between the blood ａnd the underlying tissue. Changes in endothelial cell function appear to play a key role in the pathogenesis of atherosclerosis. Endothelial cells synthesize ａnd secrete activators as well as inhibitors of both the coagulation system ａnd the fibrinolysis system in addition to mediators that influence the adhesion ａnd aggregation of blood platelets. Endothelial cells also release molecules that control cell proliferation ａnd modulate vessel wall tone [1]. Human endothelial cells produce antithrombotic ａnd thrombotic factors such as t-PA ａnd PAI-1 ａnd respond to TNF-alpha by modifying growth characteristics, producing cytokines such as GM-CSF, expressing ICAM-1 on the surface ａnd producing large amounts of nitric oxide ａnd endothelin [2]. Endothelial injury, with consequent endothelial dysfunction, is caused by percutaneous transluminal coronary angioplasty [3] ａnd may play an important role in subsequent restenosis [4]. Many of the endothelial processes can be studied in vitro using cultured cells, ａnd HCAEC will surely be very useful to studying ａnd understanding the molecular mechanisms involved in coronary artery diseases.
HCAEC from ScienCell Research Laboratories are isolated from human coronary artery. HCAEC are cryopreserved at passage one ａnd delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HCAEC are characterized by immunofluorescent method with antibodies to vWF/Factor VIII ａnd CD31 （P-CAM） ａnd by uptake of DiI-Ac-LDL. HCAEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast ａnd fungi. HCAEC are guaranteed to further expand for 15 population doublings at the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Endothelial Cell Medium （ECM, Cat. No. 1001） for the culturing of HCAEC in vitro.Product Use
HCAEC are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.Storage
Directly ａnd immediately transfer cells from dry ice to liquid nitrogen upon receiving ａnd keep the cells in liquid nitrogen until cell culture needed for experiments.
Description
Endothelial cells constitute the natural interface between the blood ａnd the underlying tissue. Changes in endothelial cell function appear to play a key role in the pathogenesis of atherosclerosis. Endothelial cells synthesize ａnd secrete activators as well as inhibitors of both the coagulation system ａnd the fibrinolysis system in addition to mediators that influence the adhesion ａnd aggregation of blood platelets. Endothelial cells also release molecules that control cell proliferation ａnd modulate vessel wall tone [1]. Human endothelial cells produce antithrombotic ａnd thrombotic factors such as t-PA ａnd PAI-1 ａnd respond to TNF-alpha by modifying growth characteristics, producing cytokines such as GM-CSF, expressing ICAM-1 on the surface ａnd producing large amounts of nitric oxide ａnd endothelin [2]. Endothelial injury, with consequent endothelial dysfunction, is caused by percutaneous transluminal coronary angioplasty [3] ａnd may play an important role in subsequent restenosis [4]. Many of the endothelial processes can be studied in vitro using cultured cells, ａnd HCAEC will surely be very useful to studying ａnd understanding the molecular mechanisms involved in coronary artery diseases.
HCAEC from ScienCell Research Laboratories are isolated from human coronary artery. HCAEC are cryopreserved at passage one ａnd delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HCAEC are characterized by immunofluorescent method with antibodies to vWF/Factor VIII ａnd CD31 （P-CAM） ａnd by uptake of DiI-Ac-LDL. HCAEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast ａnd fungi. HCAEC are guaranteed to further expand for 15 population doublings at the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Endothelial Cell Medium （ECM, Cat. No. 1001） for the culturing of HCAEC in vitro.Product Use
HCAEC are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.Storage
Directly ａnd immediately transfer cells from dry ice to liquid nitrogen upon receiving ａnd keep the cells in liquid nitrogen until cell culture needed for experiments.
Description
Endothelial cells constitute the natural interface between the blood ａnd the underlying tissue. Changes in endothelial cell function appear to play a key role in the pathogenesis of atherosclerosis. Endothelial cells synthesize ａnd secrete activators as well as inhibitors of both the coagulation system ａnd the fibrinolysis system in addition to mediators that influence the adhesion ａnd aggregation of blood platelets. Endothelial cells also release molecules that control cell proliferation ａnd modulate vessel wall tone [1]. Human endothelial cells produce antithrombotic ａnd thrombotic factors such as t-PA ａnd PAI-1 ａnd respond to TNF-alpha by modifying growth characteristics, producing cytokines such as GM-CSF, expressing ICAM-1 on the surface ａnd producing large amounts of nitric oxide ａnd endothelin [2]. Endothelial injury, with consequent endothelial dysfunction, is caused by percutaneous transluminal coronary angioplasty [3] ａnd may play an important role in subsequent restenosis [4]. Many of the endothelial processes can be studied in vitro using cultured cells, ａnd HCAEC will surely be very useful to studying ａnd understanding the molecular mechanisms involved in coronary artery diseases.HCAEC from ScienCell Research Laboratories are isolated from human coronary artery. HCAEC are cryopreserved at passage one ａnd delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HCAEC are characterized by immunofluorescent method with antibodies to vWF/Factor VIII ａnd CD31 （P-CAM） ａnd by uptake of DiI-Ac-LDL. HCAEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast ａnd fungi. HCAEC are guaranteed to further expand for 15 population doublings at the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Endothelial Cell Medium （ECM, Cat. No. 1001） for the culturing of HCAEC in vitro.Product Use
HCAEC are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.Storage
Directly ａnd immediately transfer cells from dry ice to liquid nitrogen upon receiving ａnd keep the cells in liquid nitrogen until cell culture needed for experiments.
