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人心包成纤维细胞
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更新日期：2022/8/19 17:45:54
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人心包成纤维细胞
上海义森生物科技有限公司
DescriptionPericardial Fibroblasts are the principal cell type of the serous pericardium. They help to form the strong connective tissue that allows the pericardium to serve as a protective sac for the heart. Pericardial fibroblasts are exposed to a dynamic mechanical environment, being attached to the diaphragm, which influences the structure integrity of both healthy ａnd healing tissue. Fibroblasts secrete a non-rigid extracellular matrix that is rich in type I and/or type III collagen [1] ａnd there is good evidence that fibroblasts in different parts of the body are intrinsically different [2]. Fibroblasts have been extensively used for a wide range of cellular ａnd molecular studies because they are one of easiest types of cells to grow in culture, ａnd their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection.
HPcF from ScienCell Research Laboratories are isolated from the human heart. HPcF are cryopreserved at secondary culture （passage one） ａnd are delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HPcF are characterized by immunofluorescent method with antibody to fibronectin. HPcF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast ａnd fungi. HPcF are guaranteed to further expand for 15 population doublings at the condition provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Fibroblast Medium （FM, Cat. No. 2301） for the culturing of HPcF in vitro.
Product Use
HPcF are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Directly ａnd immediately transfer cells from dry ice to liquid nitrogen upon receiving ａnd keep the cells in liquid nitrogen until cell culture needed for experiments.
Shipping
Dry ice.
Reference
[1] Gabbiani, G., Rungger-Brandle, E., The fibroblast. In Tissue Repair ａnd Regeneration （L. E. Glynn, ed.）, pp 1- 50. Handbook of Inflammation, Vol. 3. Amsterdam, Elsevier, 1981.
[2] Conrad, G. W., Hart, G. W., Chen, Y. （1977） Differences in vitro between fibroblast-like cells from cornea, heart, ａnd skin of embryonic chicks. J. Cell Sci. 26:119-137.
DescriptionPericardial Fibroblasts are the principal cell type of the serous pericardium. They help to form the strong connective tissue that allows the pericardium to serve as a protective sac for the heart. Pericardial fibroblasts are exposed to a dynamic mechanical environment, being attached to the diaphragm, which influences the structure integrity of both healthy ａnd healing tissue. Fibroblasts secrete a non-rigid extracellular matrix that is rich in type I and/or type III collagen [1] ａnd there is good evidence that fibroblasts in different parts of the body are intrinsically different [2]. Fibroblasts have been extensively used for a wide range of cellular ａnd molecular studies because they are one of easiest types of cells to grow in culture, ａnd their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection.
HPcF from ScienCell Research Laboratories are isolated from the human heart. HPcF are cryopreserved at secondary culture （passage one） ａnd are delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HPcF are characterized by immunofluorescent method with antibody to fibronectin. HPcF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast ａnd fungi. HPcF are guaranteed to further expand for 15 population doublings at the condition provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Fibroblast Medium （FM, Cat. No. 2301） for the culturing of HPcF in vitro.
Product Use
HPcF are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Directly ａnd immediately transfer cells from dry ice to liquid nitrogen upon receiving ａnd keep the cells in liquid nitrogen until cell culture needed for experiments.
Shipping
Dry ice.
Reference
[1] Gabbiani, G., Rungger-Brandle, E., The fibroblast. In Tissue Repair ａnd Regeneration （L. E. Glynn, ed.）, pp 1- 50. Handbook of Inflammation, Vol. 3. Amsterdam, Elsevier, 1981.
[2] Conrad, G. W., Hart, G. W., Chen, Y. （1977） Differences in vitro between fibroblast-like cells from cornea, heart, ａnd skin of embryonic chicks. J. Cell Sci. 26:119-137.
Pericardial Fibroblasts are the principal cell type of the serous pericardium. They help to form the strong connective tissue that allows the pericardium to serve as a protective sac for the heart. Pericardial fibroblasts are exposed to a dynamic mechanical environment, being attached to the diaphragm, which influences the structure integrity of both healthy ａnd healing tissue. Fibroblasts secrete a non-rigid extracellular matrix that is rich in type I and/or type III collagen [1] ａnd there is good evidence that fibroblasts in different parts of the body are intrinsically different [2]. Fibroblasts have been extensively used for a wide range of cellular ａnd molecular studies because they are one of easiest types of cells to grow in culture, ａnd their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection.
HPcF from ScienCell Research Laboratories are isolated from the human heart. HPcF are cryopreserved at secondary culture （passage one） ａnd are delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HPcF are characterized by immunofluorescent method with antibody to fibronectin. HPcF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast ａnd fungi. HPcF are guaranteed to further expand for 15 population doublings at the condition provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Fibroblast Medium （FM, Cat. No. 2301） for the culturing of HPcF in vitro.
Product Use
HPcF are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Directly ａnd immediately transfer cells from dry ice to liquid nitrogen upon receiving ａnd keep the cells in liquid nitrogen until cell culture needed for experiments.
Shipping
Dry ice.
Reference
[1] Gabbiani, G., Rungger-Brandle, E., The fibroblast. In Tissue Repair ａnd Regeneration （L. E. Glynn, ed.）, pp 1- 50. Handbook of Inflammation, Vol. 3. Amsterdam, Elsevier, 1981.
[2] Conrad, G. W., Hart, G. W., Chen, Y. （1977） Differences in vitro between fibroblast-like cells from cornea, heart, ａnd skin of embryonic chicks. J. Cell Sci. 26:119-137.
