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人眼睛脉络膜成纤维细胞
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更新日期：2022/8/19 17:45:54
所 在 地：美洲
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详细内容
人眼睛脉络膜成纤维细胞
上海义森生物科技有限公司
Description
Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They are one of the easiest types of cells to grow in culture ａnd their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. Fibroblasts secrete a non-rigid extracellular matrix that is rich in type I and/or type III collagen [1]. During wound healing, fibroblasts switch from a migrator, repopulation phenotype to a contractile, matrix-reassembling phenotype. Choroid fibroblasts are exposed to a dynamic environment that influences cellular VEGF expression, which plays a significant role in angiogenesis ａnd macular degeneration [2].
HOCF from ScienCell Research Laboratories are isolated from human ocular choroid tissue. HOCF are cryopreserved at primary culture. Each vial contains >5 x 10^5 cells in 1 ml volume. HOCF are characterized by their spindle morphology ａnd immunofluorescent method with antibody to fibronectin. HOCF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast ａnd fungi. HOCF are guaranteed to further expand for 15 population doublings at the condition provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Fibroblast Medium （FM, Cat. No. 2301） for the culturing of HOCF in vitro.
Product Use
HOCF are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Directly ａnd immediately transfer cells from dry ice to liquid nitrogen upon receiving ａnd keep the cells in liquid nitrogen until cell culture needed for experiments.
Shipping
Dry ice.
Reference
[1] Gabbiani, G., Rungger-Brandle, E., The fibroblast. In Tissue Repair ａnd Regeneration （L. E. Glynn, ed.）, pp 1- 50. Handbook of Inflammation, Vol. 3. Amsterdam, Elsevier, 1981.
[2] Kvanta, A. （1995） Expression ａnd regulation of vascular endothelial growth factor in choroidal fibroblasts. Curr Eye Res. 14（11）:1015-20.
Description
Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They are one of the easiest types of cells to grow in culture ａnd their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. Fibroblasts secrete a non-rigid extracellular matrix that is rich in type I and/or type III collagen [1]. During wound healing, fibroblasts switch from a migrator, repopulation phenotype to a contractile, matrix-reassembling phenotype. Choroid fibroblasts are exposed to a dynamic environment that influences cellular VEGF expression, which plays a significant role in angiogenesis ａnd macular degeneration [2].
HOCF from ScienCell Research Laboratories are isolated from human ocular choroid tissue. HOCF are cryopreserved at primary culture. Each vial contains >5 x 10^5 cells in 1 ml volume. HOCF are characterized by their spindle morphology ａnd immunofluorescent method with antibody to fibronectin. HOCF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast ａnd fungi. HOCF are guaranteed to further expand for 15 population doublings at the condition provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Fibroblast Medium （FM, Cat. No. 2301） for the culturing of HOCF in vitro.
Product Use
HOCF are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Directly ａnd immediately transfer cells from dry ice to liquid nitrogen upon receiving ａnd keep the cells in liquid nitrogen until cell culture needed for experiments.
Shipping
Dry ice.
Reference
[1] Gabbiani, G., Rungger-Brandle, E., The fibroblast. In Tissue Repair ａnd Regeneration （L. E. Glynn, ed.）, pp 1- 50. Handbook of Inflammation, Vol. 3. Amsterdam, Elsevier, 1981.
[2] Kvanta, A. （1995） Expression ａnd regulation of vascular endothelial growth factor in choroidal fibroblasts. Curr Eye Res. 14（11）:1015-20.
Description
Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They are one of the easiest types of cells to grow in culture ａnd their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. Fibroblasts secrete a non-rigid extracellular matrix that is rich in type I and/or type III collagen [1]. During wound healing, fibroblasts switch from a migrator, repopulation phenotype to a contractile, matrix-reassembling phenotype. Choroid fibroblasts are exposed to a dynamic environment that influences cellular VEGF expression, which plays a significant role in angiogenesis ａnd macular degeneration [2].
HOCF from ScienCell Research Laboratories are isolated from human ocular choroid tissue. HOCF are cryopreserved at primary culture. Each vial contains >5 x 10^5 cells in 1 ml volume. HOCF are characterized by their spindle morphology ａnd immunofluorescent method with antibody to fibronectin. HOCF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast ａnd fungi. HOCF are guaranteed to further expand for 15 population doublings at the condition provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Fibroblast Medium （FM, Cat. No. 2301） for the culturing of HOCF in vitro.
Product Use
HOCF are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Directly ａnd immediately transfer cells from dry ice to liquid nitrogen upon receiving ａnd keep the cells in liquid nitrogen until cell culture needed for experiments.
Shipping
Dry ice.
Reference
[1] Gabbiani, G., Rungger-Brandle, E., The fibroblast. In Tissue Repair ａnd Regeneration （L. E. Glynn, ed.）, pp 1- 50. Handbook of Inflammation, Vol. 3. Amsterdam, Elsevier, 1981.
[2] Kvanta, A. （1995） Expression ａnd regulation of vascular endothelial growth factor in choroidal fibroblasts. Curr Eye Res. 14（11）:1015-20.
