Description

Mesenchymal stem cells （MSC） are well-characterized population of adult stem cells. They have the potential to develop into mature cells that produce fat, cartilage, bone, tendons, ａnd muscle [1, 2]. These properties in combination with their developmental plasticity have generated tremendous interest in the potential use of mesenchymal stem cells to replace damaged tissues. MSC cultured without serum in the presence of transformation growth factor will differentiate into chondrocytes, whereas MSC cultured in serum with ascorbic acid ａnd dexamethasone will differentiate into osteoblasts. MSC has the capability for renewal ａnd differentiation into various lineages of mesenchymal tissues. In essence MSC could be cultured to expand their numbers then transplanted to the injured site or after seeding in/on shaped biomimetic scaffold to generate appropriate tissue constructs.

HVMSC from ScienCell Research Laboratories are isolated from human vertebral body. HVMSC are cryopreserved at passage one culture ａnd delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HVMSC are characterized by immunoflurescent method with antibodies to CD73, CD90, CD105 ａnd Oil Red staining after adipo-differentiation. HVMSC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast ａnd fungi. HVMSC are guaranteed to further culture at the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Mesenchymal Stem Cell Medium （MSCM, Cat. No. 7501） for the culturing of HVMSC in vitro.

Product Use

HVMSC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage

Transfer cells directly ａnd immediately from dry ice to liquid nitrogen upon receiving ａnd keep the cells in liquid nitrogen until cell culture is needed for experiments