产品展示
优质供应
详细内容
人脐静脉内皮细胞
上海义森生物科技有限公司
血管内皮细胞对维持血管动态平衡起着重要作用。它们合成、分泌凝血和纤溶系统的激活因子和抑制因子、影响血小板粘附和聚集的调节因子。内皮细胞还释放控制细胞增殖和调节血管壁紧张度的分子。这些过程都可以用培养的细胞进行体外研究，人脐静脉和动脉内皮细胞是这类研究中*常用的细胞类型。除了普通的内皮细胞特征例如“鹅卵石状”， VIII因子阳性着色，能摄取乙酰基化低密度脂蛋白，人脐血管内皮细CD31染色阳性。用IL-1或TNF-α预先处理过的细胞也能选择性地表达E-selectin和VCAM。
推荐培养基:（ECM, Cat. No. 1001）
产地：San Diego,US储存：液氮
品牌：ScienCell
运输：干冰
用途：科研
The vascular endothelial cells contribute to the maintenance of vascular homeostasis. They synthesize ａnd secrete activators as well as inhibitors of both the coagulation system ａnd the fibrinolysis system in addition to mediators that influence the adhesion ａnd aggregation of blood platelets. Endothelial cells also release molecules that control cell proliferation ａnd modulate vessel wall tone. Many of the endothelial processes can be studied in vitro using cultured cells, ａnd human umbilical vein endothelial cells （HUVEC） are the most commonly used cell type for such studies. Except for the common endothelial cell features such as "cobblestone" morphology, positive staining for Factors VIII ａnd the ability to take up acetylated low-density lipoprotein [1], HUVEC stein positively with CD-31 [2]. Cells pretreated with IL-1 or TNF-alpha also selectively express E-selectin [3] ａnd VCAM [4].
HUVEC from ScienCell Research Laboratories are isolated from human umbilical vein. HUVEC are cryopreserved at passage one ａnd delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HUVEC are characterized by immunofluorescent method with antibodies to vWF/Factor VIII ａnd CD31 （P-CAM） ａnd by uptake of DiI-Ac-LDL. HUVEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast ａnd fungi. HUVEC are guaranteed to further expand for 15 population doublings at the conditions provided by ScienCell Research Laboratories.
Reference
1. Morgan, D. M. L. （1996） Isolation ａnd culture of human umbilical vein endothelial cells. In "Human Cell Culture Protocols" （Gareth E. Jones, eds.） Humana Press, Totowa, New Jersey, pp.104-109.
2. Newman, P. J. et al., （1990） PECAM-1 （CD31） cloning ａnd relation to adhesion molecules of the immunoglobulin gene superfamily. Science 247: 1219-1222.
3. Bevilaqua, M. P. et al., （1989） Endothelial leukocyte adhesion molecule 1: an inducible receptor for neutrophils related to complement regulatory proteins ａnd lectins. Science 243:1`160-1165.
4. Osborn, L. et al., （1989） Direct cloning of vascular cell adhesion molecule 1, a cytokine induced endothelial protein that binds to lymphocytes. Cell 59:1203-1211.
血管内皮细胞对维持血管动态平衡起着重要作用。它们合成、分泌凝血和纤溶系统的激活因子和抑制因子、影响血小板粘附和聚集的调节因子。内皮细胞还释放控制细胞增殖和调节血管壁紧张度的分子。这些过程都可以用培养的细胞进行体外研究，人脐静脉和动脉内皮细胞是这类研究中*常用的细胞类型。除了普通的内皮细胞特征例如“鹅卵石状”， VIII因子阳性着色，能摄取乙酰基化低密度脂蛋白，人脐血管内皮细CD31染色阳性。用IL-1或TNF-α预先处理过的细胞也能选择性地表达E-selectin和VCAM。
推荐培养基:（ECM, Cat. No. 1001）
产地：San Diego,US储存：液氮
品牌：ScienCell
运输：干冰
用途：科研
The vascular endothelial cells contribute to the maintenance of vascular homeostasis. They synthesize ａnd secrete activators as well as inhibitors of both the coagulation system ａnd the fibrinolysis system in addition to mediators that influence the adhesion ａnd aggregation of blood platelets. Endothelial cells also release molecules that control cell proliferation ａnd modulate vessel wall tone. Many of the endothelial processes can be studied in vitro using cultured cells, ａnd human umbilical vein endothelial cells （HUVEC） are the most commonly used cell type for such studies. Except for the common endothelial cell features such as "cobblestone" morphology, positive staining for Factors VIII ａnd the ability to take up acetylated low-density lipoprotein [1], HUVEC stein positively with CD-31 [2]. Cells pretreated with IL-1 or TNF-alpha also selectively express E-selectin [3] ａnd VCAM [4].
HUVEC from ScienCell Research Laboratories are isolated from human umbilical vein. HUVEC are cryopreserved at passage one ａnd delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HUVEC are characterized by immunofluorescent method with antibodies to vWF/Factor VIII ａnd CD31 （P-CAM） ａnd by uptake of DiI-Ac-LDL. HUVEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast ａnd fungi. HUVEC are guaranteed to further expand for 15 population doublings at the conditions provided by ScienCell Research Laboratories.
Reference
1. Morgan, D. M. L. （1996） Isolation ａnd culture of human umbilical vein endothelial cells. In "Human Cell Culture Protocols" （Gareth E. Jones, eds.） Humana Press, Totowa, New Jersey, pp.104-109.
2. Newman, P. J. et al., （1990） PECAM-1 （CD31） cloning ａnd relation to adhesion molecules of the immunoglobulin gene superfamily. Science 247: 1219-1222.
3. Bevilaqua, M. P. et al., （1989） Endothelial leukocyte adhesion molecule 1: an inducible receptor for neutrophils related to complement regulatory proteins ａnd lectins. Science 243:1`160-1165.
4. Osborn, L. et al., （1989） Direct cloning of vascular cell adhesion molecule 1, a cytokine induced endothelial protein that binds to lymphocytes. Cell 59:1203-1211.
推荐培养基:（ECM, Cat. No. 1001）
产地：San Diego,US储存：液氮
品牌：ScienCell
运输：干冰
用途：科研
The vascular endothelial cells contribute to the maintenance of vascular homeostasis. They synthesize ａnd secrete activators as well as inhibitors of both the coagulation system ａnd the fibrinolysis system in addition to mediators that influence the adhesion ａnd aggregation of blood platelets. Endothelial cells also release molecules that control cell proliferation ａnd modulate vessel wall tone. Many of the endothelial processes can be studied in vitro using cultured cells, ａnd human umbilical vein endothelial cells （HUVEC） are the most commonly used cell type for such studies. Except for the common endothelial cell features such as "cobblestone" morphology, positive staining for Factors VIII ａnd the ability to take up acetylated low-density lipoprotein [1], HUVEC stein positively with CD-31 [2]. Cells pretreated with IL-1 or TNF-alpha also selectively express E-selectin [3] ａnd VCAM [4].
HUVEC from ScienCell Research Laboratories are isolated from human umbilical vein. HUVEC are cryopreserved at passage one ａnd delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HUVEC are characterized by immunofluorescent method with antibodies to vWF/Factor VIII ａnd CD31 （P-CAM） ａnd by uptake of DiI-Ac-LDL. HUVEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast ａnd fungi. HUVEC are guaranteed to further expand for 15 population doublings at the conditions provided by ScienCell Research Laboratories.
Reference
1. Morgan, D. M. L. （1996） Isolation ａnd culture of human umbilical vein endothelial cells. In "Human Cell Culture Protocols" （Gareth E. Jones, eds.） Humana Press, Totowa, New Jersey, pp.104-109.
2. Newman, P. J. et al., （1990） PECAM-1 （CD31） cloning ａnd relation to adhesion molecules of the immunoglobulin gene superfamily. Science 247: 1219-1222.
3. Bevilaqua, M. P. et al., （1989） Endothelial leukocyte adhesion molecule 1: an inducible receptor for neutrophils related to complement regulatory proteins ａnd lectins. Science 243:1`160-1165.
4. Osborn, L. et al., （1989） Direct cloning of vascular cell adhesion molecule 1, a cytokine induced endothelial protein that binds to lymphocytes. Cell 59:1203-1211.
