Description

Fibroblasts are mesenchymal cells, which are derived from the embryonic mesoderm. Fibroblasts secrete a nonrigid extracellular matrix that is rich in type I and/or type III collagen [1]. Fibroblasts have been isolated from many tissues ａnd there is good evidence that in different parts of the body, they are intrinsically different [2]. Fibroblasts exhibit a characteristic spindle morphology ａnd have a finite life span of about 50 generations in culture. Fibroblasts have been extensively used for a wide range of cellular ａnd molecular studies. This is mainly because they are one of the easiest types of cells to grow in culture ａnd their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection.

HPrF from ScienCell Research Laboratories are isolated from human prostate tissue. HPrF are cryopreserved at primary culture ａnd delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HPrF are characterized by immunofluorescent method with antibody to fibronectin. HPrF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast ａnd fungi. HPrF are guaranteed to further expand for 15 population doublings in the conditions specified by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Fibroblast Medium （FM, Cat. No. 2301） for the culturing of HPrF in vitro.

Product Use

HPrF are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage

Transfer cells directly ａnd immediately from dry ice to liquid nitrogen upon receiving ａnd keep the cells in liquid nitrogen until cell culture needed for experiments.

Shipping

Dry ice.