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大鼠脑膜细胞
上海义森生物科技有限公司
围绕大脑的脑膜细胞积极参与中枢神经系统的正常发育。它们在以下方面起着重要作用，例如稳定软膜表面细胞外基质、组织放射状胶质细胞网络和小脑皮层迭片结构。在个体发育过程中对脑膜细胞选择性的药理伤害导致大脑皮质和齿状回畸形。脑膜细胞的移植物来源于覆盖在大脑皮层上的脑膜，成年大鼠脊髓伤害促进轴索再生。体外研究表明脑膜细胞定向趋化未成熟的神经细胞，但对神经胶质细胞没有此作用。 大鼠脑膜细胞（RMC）提取于新生大鼠脑脊膜，原代冻存。每管含有细胞数>5×105 cells/ml，此细胞通过对Fibronectin、Alpha-SMA、Thy 1.1和GFAP （-） 的免疫荧光染色验证，经测试不含有支原体、细菌、酵母和真菌。细胞可以达到15倍增。
推荐培养基:（MCM, Cat. No. 1401）
产地：San Diego,US储存：液氮
品牌：ScienCell
运输：干冰
用途：科研
Meningeal cells surrounding the brain participate actively in the normal development of the central nervous system. For example, they play important roles in both stabilizing the extracellular matrix of the pial surface ａnd by organizing the radial glial scaffold ａnd the lamination of the cerebellar cortex [1]. Selective pharmacological destruction of the meningeal cells during a critical ontogenetic period leads to specific malformation of both the cerbella cortex ａnd dentate gyrus [1]. Grafts of meningeal cells, which derived form meninges overlying the cerebral cortex, in adult rat spinal cord lesion promote axonal regrowth [2]. In vitro study show that meningeal cell chemotactically orient the migration of immature neurons but not glial cells [3].
RMC from ScienCell Research Laboratories are isolated from rat leptomeningi. RMC are cryopreserved at passage one cultures ａnd delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. RMC are characterized by immunofluorescent method with antibodies to fibronectin ａnd negative to GFAP, alpha-smooth muscle actin ａnd Thy 1.1. RMC are negative for mycoplasma, bacteria, yeast ａnd fungi. RMC are guaranteed to further expand for 15 population doublings in the conditions specified by ScienCell Research Laboratories.
Reference
[1] Hartmann, D., Sievers, J. Pehlemann, F. W. ａnd Berry, M. （1992） Destruction of meningeal cells over the medial cerebral hemisphere of newborn hamster prevents the formation of the infrapyramidal blade of the dentate gyrus. J. Comparative Neurol. 320:33-61.
[2] Franzen, R., Martin, D., Daloze, A., Moonen, G. ａnd Schoenen, J. （1999） Grafts of meningeal fibroblasts in adult rat spinal cord lesion promote axonal regrowth. Neuroreport 10:1551-1556.
[3] Hartmann, D., Schulze, M. ａnd Sievers, J. （1998） Meningeal cells stimulate ａnd direct the migration of cerebellar external granule cells in vitro. J. Neurocytol. 27:395-409.
围绕大脑的脑膜细胞积极参与中枢神经系统的正常发育。它们在以下方面起着重要作用，例如稳定软膜表面细胞外基质、组织放射状胶质细胞网络和小脑皮层迭片结构。在个体发育过程中对脑膜细胞选择性的药理伤害导致大脑皮质和齿状回畸形。脑膜细胞的移植物来源于覆盖在大脑皮层上的脑膜，成年大鼠脊髓伤害促进轴索再生。体外研究表明脑膜细胞定向趋化未成熟的神经细胞，但对神经胶质细胞没有此作用。 大鼠脑膜细胞（RMC）提取于新生大鼠脑脊膜，原代冻存。每管含有细胞数>5×105 cells/ml，此细胞通过对Fibronectin、Alpha-SMA、Thy 1.1和GFAP （-） 的免疫荧光染色验证，经测试不含有支原体、细菌、酵母和真菌。细胞可以达到15倍增。
推荐培养基:（MCM, Cat. No. 1401）
产地：San Diego,US储存：液氮
品牌：ScienCell
运输：干冰
用途：科研
Meningeal cells surrounding the brain participate actively in the normal development of the central nervous system. For example, they play important roles in both stabilizing the extracellular matrix of the pial surface ａnd by organizing the radial glial scaffold ａnd the lamination of the cerebellar cortex [1]. Selective pharmacological destruction of the meningeal cells during a critical ontogenetic period leads to specific malformation of both the cerbella cortex ａnd dentate gyrus [1]. Grafts of meningeal cells, which derived form meninges overlying the cerebral cortex, in adult rat spinal cord lesion promote axonal regrowth [2]. In vitro study show that meningeal cell chemotactically orient the migration of immature neurons but not glial cells [3].
RMC from ScienCell Research Laboratories are isolated from rat leptomeningi. RMC are cryopreserved at passage one cultures ａnd delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. RMC are characterized by immunofluorescent method with antibodies to fibronectin ａnd negative to GFAP, alpha-smooth muscle actin ａnd Thy 1.1. RMC are negative for mycoplasma, bacteria, yeast ａnd fungi. RMC are guaranteed to further expand for 15 population doublings in the conditions specified by ScienCell Research Laboratories.
Reference
[1] Hartmann, D., Sievers, J. Pehlemann, F. W. ａnd Berry, M. （1992） Destruction of meningeal cells over the medial cerebral hemisphere of newborn hamster prevents the formation of the infrapyramidal blade of the dentate gyrus. J. Comparative Neurol. 320:33-61.
[2] Franzen, R., Martin, D., Daloze, A., Moonen, G. ａnd Schoenen, J. （1999） Grafts of meningeal fibroblasts in adult rat spinal cord lesion promote axonal regrowth. Neuroreport 10:1551-1556.
[3] Hartmann, D., Schulze, M. ａnd Sievers, J. （1998） Meningeal cells stimulate ａnd direct the migration of cerebellar external granule cells in vitro. J. Neurocytol. 27:395-409.
大鼠脑膜细胞（RMC）提取于新生大鼠脑脊膜，原代冻存。每管含有细胞数>5×105 cells/ml，此细胞通过对Fibronectin、Alpha-SMA、Thy 1.1和GFAP （-） 的免疫荧光染色验证，经测试不含有支原体、细菌、酵母和真菌。细胞可以达到15倍增。
推荐培养基:（MCM, Cat. No. 1401）
产地：San Diego,US储存：液氮
品牌：ScienCell
运输：干冰
用途：科研
Meningeal cells surrounding the brain participate actively in the normal development of the central nervous system. For example, they play important roles in both stabilizing the extracellular matrix of the pial surface ａnd by organizing the radial glial scaffold ａnd the lamination of the cerebellar cortex [1]. Selective pharmacological destruction of the meningeal cells during a critical ontogenetic period leads to specific malformation of both the cerbella cortex ａnd dentate gyrus [1]. Grafts of meningeal cells, which derived form meninges overlying the cerebral cortex, in adult rat spinal cord lesion promote axonal regrowth [2]. In vitro study show that meningeal cell chemotactically orient the migration of immature neurons but not glial cells [3].
RMC from ScienCell Research Laboratories are isolated from rat leptomeningi. RMC are cryopreserved at passage one cultures ａnd delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. RMC are characterized by immunofluorescent method with antibodies to fibronectin ａnd negative to GFAP, alpha-smooth muscle actin ａnd Thy 1.1. RMC are negative for mycoplasma, bacteria, yeast ａnd fungi. RMC are guaranteed to further expand for 15 population doublings in the conditions specified by ScienCell Research Laboratories.
Reference
[1] Hartmann, D., Sievers, J. Pehlemann, F. W. ａnd Berry, M. （1992） Destruction of meningeal cells over the medial cerebral hemisphere of newborn hamster prevents the formation of the infrapyramidal blade of the dentate gyrus. J. Comparative Neurol. 320:33-61.
[2] Franzen, R., Martin, D., Daloze, A., Moonen, G. ａnd Schoenen, J. （1999） Grafts of meningeal fibroblasts in adult rat spinal cord lesion promote axonal regrowth. Neuroreport 10:1551-1556.
[3] Hartmann, D., Schulze, M. ａnd Sievers, J. （1998） Meningeal cells stimulate ａnd direct the migration of cerebellar external granule cells in vitro. J. Neurocytol. 27:395-409.
