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大鼠淋巴成纤维细胞
上海义森生物科技有限公司
成纤维细胞是胚胎中胚层起源的间叶细胞。它们被广泛地用于细胞和分子方面的研究。这主要是因为纤维原细胞是体外培养中*容易生长的一类细胞，它们的耐久力使它们能够用于基因转染、显微注射等多种操作。有确凿的证据表明，在身体不同的部分的成纤维细胞有显著的不同。组织中的成纤维细胞暴露在动态的机械环境中将影响健康的和正在愈合的软组织的结构完整性。纤维原细胞分泌富含I 和，或III型胶原的细胞外基质。另外，有报道指出在淋巴增生疾病中脾脏成纤维细胞大量增长。
推荐培养基:（MM, Cat. No. 1901）
储存：液氮
运输：干冰
用途：科研
Cell Specification
Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular ａnd molecular studies. This is mainly because they are one of easiest types of cells to grow in culture, ａnd their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. There is good evidence that fibroblasts in different parts of the body are intrinsically different [1]. Fibroblasts within tissues are exposed to a dynamic mechnical environment, which influences the structure integrity of both healthy ａnd healing soft tissue. Fibroblasts secrete a nonrigid extracellular matrix that is rich in type I and/or type III collagen [2]. Besides, it has been reported that the growth of splenic fibroblasts has been increased in lymphoproliferatived diseases [3].
RLF from ScienCell Research Laboratories are isolated from rat lymph nodes. RLF are cryopreserved at primary culture ａnd delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. RLF are characterized by their spindle morphology ａnd immunofluorescent method with antibody to fibronectin. RLF are negative mycoplasma, bacteria, yeast ａnd fungi. RLF are guaranteed to further expand for 15 population doublings at the condition provided by ScienCell Research Laboratories.
Reference
[1] Conrad, G. W., Hart, G. W., Chen, Y. （1977） Differences in vitro between fibroblast-like cells from cornea, heart, ａnd skin of embryonic chicks. J. Cell Sci. 26:119-137.
[2] Gabbiani, G., Rungger-Brandle, E., The fibroblast. In Tissue Repair ａnd Regeneration （L. E. Glynn, ed.）, pp 1-50. Handbook of Inflammation, Vol. 3. Amsterdam, Elsevier, 1981.
[3] Domracheva, E. V., Rogova, E. M., Vorob＇ev, A. L. （1984） Monolayer culture of bone marrow ａnd splenic fibroblasts in lymphoproliferative diseases. Ter Avkh. 56:63-65.
成纤维细胞是胚胎中胚层起源的间叶细胞。它们被广泛地用于细胞和分子方面的研究。这主要是因为纤维原细胞是体外培养中*容易生长的一类细胞，它们的耐久力使它们能够用于基因转染、显微注射等多种操作。有确凿的证据表明，在身体不同的部分的成纤维细胞有显著的不同。组织中的成纤维细胞暴露在动态的机械环境中将影响健康的和正在愈合的软组织的结构完整性。纤维原细胞分泌富含I 和，或III型胶原的细胞外基质。另外，有报道指出在淋巴增生疾病中脾脏成纤维细胞大量增长。
推荐培养基:（MM, Cat. No. 1901）
储存：液氮
运输：干冰
用途：科研
Cell Specification
Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular ａnd molecular studies. This is mainly because they are one of easiest types of cells to grow in culture, ａnd their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. There is good evidence that fibroblasts in different parts of the body are intrinsically different [1]. Fibroblasts within tissues are exposed to a dynamic mechnical environment, which influences the structure integrity of both healthy ａnd healing soft tissue. Fibroblasts secrete a nonrigid extracellular matrix that is rich in type I and/or type III collagen [2]. Besides, it has been reported that the growth of splenic fibroblasts has been increased in lymphoproliferatived diseases [3].
RLF from ScienCell Research Laboratories are isolated from rat lymph nodes. RLF are cryopreserved at primary culture ａnd delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. RLF are characterized by their spindle morphology ａnd immunofluorescent method with antibody to fibronectin. RLF are negative mycoplasma, bacteria, yeast ａnd fungi. RLF are guaranteed to further expand for 15 population doublings at the condition provided by ScienCell Research Laboratories.
Reference
[1] Conrad, G. W., Hart, G. W., Chen, Y. （1977） Differences in vitro between fibroblast-like cells from cornea, heart, ａnd skin of embryonic chicks. J. Cell Sci. 26:119-137.
[2] Gabbiani, G., Rungger-Brandle, E., The fibroblast. In Tissue Repair ａnd Regeneration （L. E. Glynn, ed.）, pp 1-50. Handbook of Inflammation, Vol. 3. Amsterdam, Elsevier, 1981.
[3] Domracheva, E. V., Rogova, E. M., Vorob＇ev, A. L. （1984） Monolayer culture of bone marrow ａnd splenic fibroblasts in lymphoproliferative diseases. Ter Avkh. 56:63-65.
推荐培养基:（MM, Cat. No. 1901）
储存：液氮
运输：干冰
用途：科研
Cell Specification
Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular ａnd molecular studies. This is mainly because they are one of easiest types of cells to grow in culture, ａnd their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. There is good evidence that fibroblasts in different parts of the body are intrinsically different [1]. Fibroblasts within tissues are exposed to a dynamic mechnical environment, which influences the structure integrity of both healthy ａnd healing soft tissue. Fibroblasts secrete a nonrigid extracellular matrix that is rich in type I and/or type III collagen [2]. Besides, it has been reported that the growth of splenic fibroblasts has been increased in lymphoproliferatived diseases [3].
RLF from ScienCell Research Laboratories are isolated from rat lymph nodes. RLF are cryopreserved at primary culture ａnd delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. RLF are characterized by their spindle morphology ａnd immunofluorescent method with antibody to fibronectin. RLF are negative mycoplasma, bacteria, yeast ａnd fungi. RLF are guaranteed to further expand for 15 population doublings at the condition provided by ScienCell Research Laboratories.
Reference
[1] Conrad, G. W., Hart, G. W., Chen, Y. （1977） Differences in vitro between fibroblast-like cells from cornea, heart, ａnd skin of embryonic chicks. J. Cell Sci. 26:119-137.
[2] Gabbiani, G., Rungger-Brandle, E., The fibroblast. In Tissue Repair ａnd Regeneration （L. E. Glynn, ed.）, pp 1-50. Handbook of Inflammation, Vol. 3. Amsterdam, Elsevier, 1981.
[3] Domracheva, E. V., Rogova, E. M., Vorob＇ev, A. L. （1984） Monolayer culture of bone marrow ａnd splenic fibroblasts in lymphoproliferative diseases. Ter Avkh. 56:63-65.
