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小鼠胚胎成纤维细胞
点击次数：266发布时间：2013/4/26 14:40:14
更新日期：2022/8/19 17:45:54
所 在 地：美洲
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详细内容
小鼠胚胎成纤维细胞
上海义森生物科技有限公司
Description
Mouse Embryonic Fibroblasts （MEF） are used to support the growth of undifferentiated mouse or human ES cells ａnd iPS cells [1]. They provide both a substrate for the ES cells to grow on ａnd secrete many factors necessary for ES cells to maintain pluripotency. MEF cells are isolated from mouse embryos ａnd are used at their early passages [2]. Before MEF cells are used as feeder cells, they must be treated by irradiation or mitomycin C to stop the cells from further dividing.
MEF from ScienCell Research Laboratories are isolated from day 13 mouse embryos. MEF are cryopreserved at primary culture ａnd delivered frozen. Each vial contains 5 x 10^6 cells in 1 ml volume. MEF are characterized by immunofluorescent method with antibodies to fibronectin. MEF are negative for mycoplasma, bacteria, yeast ａnd fungi. MEF are guaranteed to further culture over 5 population doublings at the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use DMEM with 10% FBS for the culturing of MEF in vitro.
Product Use
MEF are used as feeder layer in mouse ａnd human ES or iPS cell culture. They are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Directly ａnd immediately transfer cells from dry ice to liquid nitrogen upon receiving ａnd keep the cells in liquid nitrogen until cell culture is needed for experiments.
Shipping
Dry ice.
Description
Mouse Embryonic Fibroblasts （MEF） are used to support the growth of undifferentiated mouse or human ES cells ａnd iPS cells [1]. They provide both a substrate for the ES cells to grow on ａnd secrete many factors necessary for ES cells to maintain pluripotency. MEF cells are isolated from mouse embryos ａnd are used at their early passages [2]. Before MEF cells are used as feeder cells, they must be treated by irradiation or mitomycin C to stop the cells from further dividing.
MEF from ScienCell Research Laboratories are isolated from day 13 mouse embryos. MEF are cryopreserved at primary culture ａnd delivered frozen. Each vial contains 5 x 10^6 cells in 1 ml volume. MEF are characterized by immunofluorescent method with antibodies to fibronectin. MEF are negative for mycoplasma, bacteria, yeast ａnd fungi. MEF are guaranteed to further culture over 5 population doublings at the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use DMEM with 10% FBS for the culturing of MEF in vitro.
Product Use
MEF are used as feeder layer in mouse ａnd human ES or iPS cell culture. They are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Directly ａnd immediately transfer cells from dry ice to liquid nitrogen upon receiving ａnd keep the cells in liquid nitrogen until cell culture is needed for experiments.
Shipping
Dry ice.
Description
Mouse Embryonic Fibroblasts （MEF） are used to support the growth of undifferentiated mouse or human ES cells ａnd iPS cells [1]. They provide both a substrate for the ES cells to grow on ａnd secrete many factors necessary for ES cells to maintain pluripotency. MEF cells are isolated from mouse embryos ａnd are used at their early passages [2]. Before MEF cells are used as feeder cells, they must be treated by irradiation or mitomycin C to stop the cells from further dividing.
MEF from ScienCell Research Laboratories are isolated from day 13 mouse embryos. MEF are cryopreserved at primary culture ａnd delivered frozen. Each vial contains 5 x 10^6 cells in 1 ml volume. MEF are characterized by immunofluorescent method with antibodies to fibronectin. MEF are negative for mycoplasma, bacteria, yeast ａnd fungi. MEF are guaranteed to further culture over 5 population doublings at the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use DMEM with 10% FBS for the culturing of MEF in vitro.
Product Use
MEF are used as feeder layer in mouse ａnd human ES or iPS cell culture. They are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Directly ａnd immediately transfer cells from dry ice to liquid nitrogen upon receiving ａnd keep the cells in liquid nitrogen until cell culture is needed for experiments.
Shipping
Dry ice.
