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CHO(中国仓鼠卵巢细胞)
上海义森生物科技有限公司
ATCC® Number: CRL-2092™ Designations: CHO-CD36 Biosafety Level: 1 Shipped: frozen
CHO(中国仓鼠卵巢细胞)
上海义森生物科技有限公司
ATCC® Number: CRL-2092™ Designations: CHO-CD36 Biosafety Level: 1 Shipped: frozen
CHO(中国仓鼠卵巢细胞)
上海义森生物科技有限公司
ATCC® Number: | CRL-2092™ |
Designations: | CHO-CD36 |
Biosafety Level: | 1 |
Shipped: | frozen |
CHO(中国仓鼠卵巢细胞)
上海义森生物科技有限公司
CHO(中国仓鼠卵巢细胞) Medium & Serum: See Propagation Growth Properties: adherent Organism: Cricetulus griseus (hamster, Chinese) Morphology: epithelial Source: Organ: ovary Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. Gender: female Comments: The CHO-CD36 cell line was constructed at DNAX Research Institute, Palo Alto, California by transfecting Chinese hamster ovary (CHO) cells with linearized cDNA of human CD36 in pCDM8 by electroporation in a 10 fold molar excess over pBJ1-Neo. Stable transfectants were then selected by growth in culture medium containing G418. CHO-CD36 cells express high levels of human CD36 (a ligand for erythrocytes infected with the human malaria parasite, Plasmodium falciparum). The cells can be used in adherence assays as a target cell for malaria infected erythrocytes. Functional expression of the transfected receptor molecules on CHO-CD36 was confirmed using parasites with different cytoadherence phenotypes and cytoadherence inhibition experiments with a panel of anti-CD36 antibodies. The use of isolates obtained from malaria patients in The Gambia confirmed the applicability of using these cells in assays for laboratory studies. A culture submitted to the ATCCin August 1993 was found to be contaminated with mycoplasma, and the line was cured by a 21 day treatment with BM Cycline.
上海义森生物科技有限公司