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德国QIAGEN试剂盒，12181 QIAGEN Plasmid Mega Kit （5）价格
产地：QIAGEN
货号：12181
名称：QIAGEN Plasmid Mega Kit
| 货号 | 产品名称 | 厂家 | 规格 | 单价 | 详细 | 订购 |
|---|---|---|---|---|---|---|
| 12181 | QIAGEN Plasmid Mega Kit （5） | Qiagen | 010-57269780 |
Procedure
With QIAGEN Plasmid Kits, bacterial lysates are cleared by centrifugation. The cleared lysate is then loaded onto the anion-exchange tip where plasmid DNA selectively binds under appropriate low-salt ａnd pH conditions. RNA, proteins, metabolites, ａnd other low-molecular-weight impurities are removed by a medium-salt wash, ａnd ultrapure plasmid DNA is eluted in high-salt buffer （see flowchart "Comparison of QIAGEN Plasmid Kit Procedures"）. The DNA is concentrated ａnd desalted by isopropanol precipitation ａnd collected by centrifugation.
Applications
Plasmid DNA purified with QIAGEN Plasmid Kits is ideal for use in applications such as transfection （see figure "Transfection Efficiency vs Plasmid Purification Method"）, cloning, manual or automated sequencing, including capillary sequencing, ａnd in vitro transcription.
Principle
The unique anion-exchange resin in QIAGEN-tips is developed exclusively for the purification of nucleic acids. Its exceptional separation properties result in DNA purity equivalent or superior to that obtained by two successive rounds of CsCl gradient centrifugation （see figure "Ultrapure Plasmid DNA Yielded by QIAGEN Plasmid Kits"）. Pre-packed QIAGEN-tips （see figure "QIAGEN Plasmid Kit Anion-Exchange Tips"） operate by gravity flow ａnd never run dry, minimizing the hands-on time required for plasmid preparation. The entire QIAGEN plasmid purification system avoids the use of toxic substances such as phenol, chloroform, ethidium bromide, ａnd CsCl, minimizing hazard both to the user ａnd the environment.
QIAGEN Plasmid Mega Kit
纯化产量可达 2.5 mg的转染级别纯度的质粒或科斯质粒DNA
纯度相当于 2倍氯化铯梯度离心
高重现性的产量和转染级别纯度
经济实惠
使用LyseBlue，获得*佳的裂解和的DNA产量
传统的QIAGEN Plasmid Giga Kits应用重力流阴离子交换柱QIAGEN-tip 2500 高效纯化质粒DNA，也单独提供QIAGEN-tips 2500。
QIAGEN Plasmid Mega Kit 特性
预期产量 柱子容量 培养体积
Up to 2.5 mg 2.5 mg 500 ml - 2.5 liters
实际产量取决于质粒拷贝数、插入片段大小、宿主菌、培养基和培养体积。
