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RPMI-1640(添加NaHCO3 1.5g/L, glucose 2.5g/L, Sodium Pyruvate 0.11g/L),90%;优质胎牛血清,10%
气相:空气,95%;二氧化碳,5%
温度:37摄氏度
细胞生长:贴壁生长
细胞形态:上皮细胞样
细胞数量:1×106个
细胞传代:2~3天换液并传代1次
细胞特性:The WM35 line was established from a primary superficial spreading melanoma (SSM) in radial growth phase (RGP)/vertical (VGP) growth phase taken from the scalp and neck of a patient on 11/17/78.
细胞纯度:93%
细胞活力:87%(Viability by Trypan Blue Exclusion)
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌
细胞冻存:液氮冻存(基础培养基+10%DMSO+20%FBS)
细胞运输:干冰运输(1 Vial)或活细胞运输(T-25 flasks)
细胞用途:只可用于科研,不可用于临床诊断和治疗
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培养条件:
RPMI-1640(添加NaHCO3 1.5g/L, glucose 2.5g/L, Sodium Pyruvate 0.11g/L),90%;优质胎牛血清,10%
气相:空气,95%;二氧化碳,5%
温度:37摄氏度
细胞生长:贴壁生长
细胞形态:上皮细胞样
细胞数量:1×106个
细胞传代:2~3天换液并传代1次
细胞特性:The WM35 line was established from a primary superficial spreading melanoma (SSM) in radial growth phase (RGP)/vertical (VGP) growth phase taken from the scalp and neck of a patient on 11/17/78.
细胞纯度:93%
细胞活力:87%(Viability by Trypan Blue Exclusion)
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌
细胞冻存:液氮冻存(基础培养基+10%DMSO+20%FBS)
细胞运输:干冰运输(1 Vial)或活细胞运输(T-25 flasks)
细胞用途:只可用于科研,不可用于临床诊断和治疗