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UM-UC-3, 人膀胱移行细胞癌
MEM培养基(添加NaHCO3 1.5g/L,Sodium Pyruvate 0.11g/L),90%;优质胎牛血清,10%
气相:空气,95%;二氧化碳,5%
温度:37摄氏度
细胞生长:贴壁生长
细胞形态:上皮细胞样,多角
细胞数量:1×106个
细胞传代:1:4~1:10传代;每周换液2~3次
细胞特性:This is a hypertriploid human cell line. The modal chromosome number was 80, occurring in 42% of cells. Cells with 78 chromosomes also occurred at a high frequency. The rate of cells with higher ploidies was 2.5%. There were 30 or more marker chromosomes in each cell. They included der(1)t(1;?) (p32;?), ?t(1p5p), i(3q), t(7q14q), ?t(2p3p) and others. The X and N3 had single copy per cell, and others were generally two to three copies per cell.
细胞纯度:93%
细胞活力:87%(Viability by Trypan Blue Exclusion)
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌
细胞冻存:液氮冻存(基础培养基+10%DMSO+20%FBS)
细胞运输:干冰运输(1 Vial)或活细胞运输(T-25 flasks)
细胞用途:只可用于科研,不可用于临床诊断和治疗