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BPH-1, 人前列腺增生细胞
RPMI-1640(添加1 ug/ml insulin, 10 ng/ml hydrocortisone, 5 ug/ml transferrin, 1 ug/ml sodium folate, 50 ng/ml phosphorylethanolamine, 5 ug/ml ascorbic acid, 1 ul/ml GIBCO trace element solution, 5 ng/ml EGF, 50 ng/ml cholera toxin and Pen-Strep (Penicillin, 100 U/ml, Streptomycin 100 mg/ml ) antibiotic),97.5%;优质胎牛血清,2.5%
气相:空气,95%;二氧化碳,5%
温度:37摄氏度
细胞生长:贴壁生长
细胞形态:卵圆形
细胞数量:1×106个
细胞传代:1:3~1:8传代;2~3天换液1次
细胞特性:The development and characterization of an epithelial cell line (BPH-1) from human prostate tissue was obtained by transurethral resection. The patient was 68 years old when the cells were collected.
细胞纯度:93%
细胞活力:87%(Viability by Trypan Blue Exclusion)
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌
细胞冻存:液氮冻存(基础培养基+10%DMSO+20%FBS)
细胞运输:干冰运输(1 Vial)或活细胞运输(T-25 flasks)
细胞用途:只可用于科研,不可用于临床诊断和治疗