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试剂盒产品详细检测范围说明书，随货附送。齐一生物销售：021－6034 8496；181214 53965；173021 04490齐一生物专业代理多种品牌进口原装检测试剂盒。.齐一生物科技有限公司作为酶联免疫金牌供应商,凭借精湛的技术,全方位的售前售后免费代测技术服务,共同铸就高品质的ELISA产品.多年专业酶免服务,质量保证,欢迎订购咨询服务.

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ELISA试剂盒常遇见问题及解答

【人葡萄糖激酶（GCK）ELISA试剂盒】1。我必须运行所有的标准和样品的重复？

是的，重复的运行，以监测测定的精度和提高检测结果的信心。

2。我必须在一次运行所有的样品吗？

不，每个试剂盒采用stripwell微孔板。这允许用户在不同的时间来分析不同数量的样品。

3。什么类型的可重复性的结果，得到与分析？

每一个工具都有一个包含一个典型数据的图表的手册。在运营商的任何变化，移液和洗涤技术，培养时间、温度、试剂盒的年龄可以引起变化的结果。每个用户都应该得到自己的标准曲线。

4。是否有可能储存的试剂比表示？

在不推荐使用条件下的部件组件的存储，以保证测试的正确性能。

5。我应该如何储存我的样品？

样品应保存在-20°C或更低的温度。长期储存，建议冻结他们在- 70余。

6。我可以修改协议吗？

BG的ELISA试剂盒进行了优化，提供*佳的结果。修改的格式或协议可能会给出错误的结果。

7。我可以使用一个不建议在试剂盒插入的样本类型吗？

该试剂盒已被验证在试剂盒插入的样品类型。未经检验的样品类型。为进一步信息的联系技术服务。

8。我的样本产生的值，在动态范围内的测定。我可以使用这些值吗？

建议只有在标准曲线的范围内下降的样本值。标准曲线的范围内的值一般是非线性的，这可能导致不正确的外推值。产生值高于标准的样品应为（进一步）稀释和重复。如果样品低于该测定的范围内，该样品被认为是不可检测的。

9。我每次都要运行一个空白或零标准吗？

是的，这些都是必需的计算，并反映任何微妙但显着的性能变化，从一天到一天，检测到检测。当排除特定的检测问题的来源时，它们也非常有帮助。

10。我能改变我在检测中使用的样本量吗？

不建议你改变卷由于BG工具被设计为*佳的性能在给定的体积

【人葡萄糖激酶（GCK）ELISA试剂盒】尊敬的客户您好！如需技术支持辅导欢迎拨打齐一生物销售：021－6034 8496；181214 53965；173021 0449011。可以使用不同的组件组件吗？

每一个组件包含有特定批号的组件，以确保所有的组件都进行优化，以及与组件中的所有其他组件。对这些特定的地段进行质量检验。这是从来没有建议使用你自己的组件或组件从其他包或供应商。

12。我的标准曲线看起来很好，但我没有得到一个信号，我的样本，当我预期，为什么？

样品不包含被分析物。一个矩阵的效果可能是掩盖检测。确保推荐的稀释液在试剂盒插入后按规定进行。如果建议稀释，请检查是否正确进行稀释。过稀释会导致样品在标准曲线的范围内下降。

【人葡萄糖激酶（GCK）ELISA试剂盒】13。你怎么推荐我洗盘子？

如果您使用的是一个自动平板洗衣机，我们建议定期检查校准，并将该系统在洗涤前冲洗缓冲液冲洗。手动洗衣机也是一样的。一个中继器或洗瓶也可以使用。用户应小心，以确保所有的内容都是送气和板拍干的无尘纸。

14。我需要用一个盘子吗？

可靠的结果可以得到无板振动，但外径的一般会低于那些使用平板振动器。

15。为什么我要用波长校正450-570nm之间？

对于酶联免疫吸附试验，在双波长读的是做正确的光密度贡献的塑料以及，灯和光学波动。

16。如果我提我的样本，我还需要遵循推荐的稀释液试剂盒中插入了吗？

提取过程中所需的样本稀释量将受到影响的纯化和浓度的影响的协议使用。稀释或浓缩的金额将由*终用户决定。

17。什么是预期的分析物浓度，我应该找到？

一个给定的分析物的量可能会有所不同，不仅从物种的物种，但也组织和细胞来源。这个信息的*佳来源是目前的文献，很容易通过互联网在多个科学数据库访问。

18。  我光密度稍高（或低）比，是与我的试剂盒说明书。为什么？

试剂盒的光密度的物理条件，如时间和温度的影响。我们建议您缩短或延长*终孵化与基板解决方案，以补偿。

【人葡萄糖激酶（GCK）ELISA试剂盒】19。  高背景的原因是什么？

1）     洗涤不当：检查洗涤液储量和确保所有建议的洗涤步骤进行。

2）     污染基材：确保没有污染的金属离子或氧化剂的基质，使用前。在酶联免疫吸附底物的开发时间内，保持额外的底物溶液。

3）暴露于光     基材：曝光可能会导致在一个蓝色的基板。在黑暗中保存（瓶）的解决方案，直到准备好进入板。

4）     错的孵育时间/温度：一般按照测试协议对于培养时间和温度。然而，如果所有的威尔斯是强烈的和同样颜色的标准稀释系列中观察到的强度梯度，那么它可能是必要的观察到的颜色的底物反应，以阻止反应更快。

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9241824Pyro Q96 MD, Full AgreementThe Full Agreement, PyM provides full service coverage for the PyroMark instrument. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one on-site inspection service, with costs for labor ａnd parts covered. With a Full Agreement, there is a response time of 2 working days.CNY

9241815Pyro Q96 ID, PM AgreementCNY

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9240678Bimba Rotary Actuator, APCNY

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9241763QIAgility, Full Agreement"Repair service delivery: onsite

Service response time (repair): 48 hours/2 business days

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for repair parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) or PC repair provision: not included

Transportation cost coverage: not applicable"CNY

9241372Reflector, UV lamp, EZ1aCNY

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Periodic inspection/maintenance: yes

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts : not included

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage"CNY

9243045Bracket, rear, transparent panel, QsymCNY

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9242764QS RGQ, Premium Agreement"Repair service delivery QIAsymphony SP/AS: onsite

Service response time (repair) QIAsymphony: 24 hours/1 business day

Repair service delivery RGQ: send to Regional Repair Center

Service response time (repair) RGQ: 7-10 days turnaround time

Periodic inspection/maintenance: yes, 1/agreement year

Cost coverage for Repair Parts : yes, full coverage

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision RGQ: yes, included (provision time 2-3 days)

Transportation cost coverage: yes, full coverage

QIAsymphony Response Time starts when customer inquiry is received by local tech service ends when a service specialist is onsite.

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Service response time (repair) 7-10 days turnaround time

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: 

Cost coverage for Repair Parts : yes, full coverage

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: yes, included (provision time 2-3 days)

Transportation cost coverage: yes, full coverage"CNY