【友情提示】：本产品仅供科研研究使用，不得用于人体临床直接检测。避免给您带来不必要的损失，请仔细阅读购买说明！

本试剂盒仅供研究使用，齐一生物销售：021－6034 8496；181214 53965；173021 04490欢迎选购！

操作步骤

1.准备试剂，样品和标准品

2.加入准备好的样品和标准品，37℃反应30分钟

3.洗板5次，加入酶标试剂，37℃反应30分钟

4.洗板5次，加入显色液A、B，37℃显色15分钟

5.加入终止液

6.15分钟之内读OD值

7.计算
齐一生物供应ELISA试剂盒检测的目的是为实验提供准确可靠的定量分析实验依据。为了保证实验数据的可靠性，在实验过程中必须坚持全面质量控制和全过程质量控制。在收集标本前都必须有一个完整的计划。

【人布氏杆菌病（brucellosis）ELISA试剂盒】样本保存条件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6个月

3.-50℃以下的冰箱，可以放一年

【人布氏杆菌病（brucellosis）ELISA试剂盒】样本收集

1.血清样本用干燥管（红色）或者促凝管（黄色）收集

2.血浆用抗凝管，一般有EDTA抗凝（紫色）、枸橼酸钠抗凝（黑色或者蓝色）

3.动物组织匀浆（细胞）：

              1g组织加入9ml PBS（浓度为0.1M，pH为7.4的）测分泌型指标

              1g组织加入1ml组织裂解液加入8ml PBS 测细胞内

粪便样本：1g样本+9ml PBS（浓度为0.01M，pH为7.4的）

没有PBS的话也可以用一下生理盐水，还是用PBS

【人布氏杆菌病（brucellosis）ELISA试剂盒】样本离心转速及时间

1.试管采集的样本：3000转，离心五分钟取上清

2.离心管或者EP管采集的样本：5000－－8000转3分钟取上清

3..样本保存－－如果样品不立即使用，应将其分成小部分-70 ℃保存，避免反复冷冻。尽可能的不要使用溶血或高血脂血。如果血清中大量颗粒，检测前先离心或过滤。不要在37℃或更高的温度加热解冻。应在室温下解冻并确保样品均匀地充分解冻。

【人布氏杆菌病（brucellosis）ELISA试剂盒】以上样本采集及处理方式供您参考，如有问题请来电，齐一生物销售：021－6034 8496；181214 53965；173021 04490

51162QIAamp 96 DNA Blood Kit (12)For 12 x 96 DNA preps: 12 QIAamp 96 Plates, QIAGEN Protease, Reagents, Buffers, Lysis Blocks, Tape Pads, Collection VesselsCNY

50112QIAamp UCP PurePathogen Blood Kit (10)CNY

133104NeXtalStock Sodium acet.  (200)CNY

9015468Sensor, photo, PM-L24CNY

9001871EZ1 Advanced, SystemEZ1 Advanced, System combines the EZ1 Advanced system ａnd installation. The Installation ａnd Introductory Training is an on-site service, conducted by a QIAGEN Service Specialist. It provides the user with a training on operation ａnd user maintenance. All travel ａnd labor cost included for Installation The installation includes labor ａnd travel costs. The performance of the installation process is documented on a GMP/GLP-compliant Field Service document.CNY

9018458Screw, w finishing washer (4), QXCNY

9018447Purge Tool, cartridge, QXCNY

9012412Incubator Tower (65°) for Plato 3 holdCNY

9012392Bottle, waste, 10 l, BR8000CNY

205111Omniscript RT Kit (50)For 50 reverse-transcription reactions: 200 units Omniscript Reverse Transcriptase, 10x Buffer RT, dNTP Mix,* RNase-free waterCNY

9018425Door, sample, QXCNY

9002123QIAxcel Advanced SystemQIAxcel Advanced System combines the QIAxcel Advanced instrument ａnd installation. The Installation ａnd Introductory Training is an on-site service, conducted by a QIAGEN Service Specialist. It provides the user with training on operation ａnd user maintenance. All travel ａnd labor cost included for Installation. Performance of the installation process is documented on a GMP/GLP-compliant Field Service document.CNY

57714QIAamp MinElute Virus Vacuum Kit (50)For 50 minipreps: 50 QIAamp MinElute Columns, QIAGEN Protease, Carrier RNA, Buffers, Extension Tubes (3 ml), Collection Tubes (1.5 ml)CNY

972012PyroMark Q96 CpG p16 (96)For quantification of methylation level in p16CNY

133227NeXtalStock Sodium cacodyl. pH 6.0 (100)CNY

301108PolyFect Transfection Reagent (100 ml)For 2500-6500 transfections in 60 mm dishes or 5000-10,000 transfections in 6-well plates.CNY

56304QIAamp DNA Micro Kit (50)For 50 DNA preps: 50 QIAamp MinElute Columns, Proteinase K, Carrier RNA, Buffers, Collection Tubes (2 ml)CNY

9002031Rotor-Gene Q MDx 5Plex HRM no PCCNY

9002018Rotor-Gene Q MDx 2plex HRM, PriorityRotor-Gene Q 2plex HRM, Priority includes the Rotor-Gene Q system, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with on site introductory training. Priority package extends warranty period of the system to 2 years with shipment to Repair Center Service has response turnaround time (repair) 7-10 days. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Replacement system (Loaner) provision included (provision time 2-3 days) with transportation cost coverage. The service package includes all parts, labor ａnd travel costs with installation performance ａnd service visits documented on a GMP/GLP-compliant Field Service documents.CNY

970902PyroMark Q24 Advanced Kit (4x24)CNY

133211NeXtalStock Magnesium chloride H2O (200)CNY

133195NeXtalStock Sodium tartrate pH 5.6 (200)CNY

293023mericon Corn (24)CNY

292055mericon Goat Kit (96)CNY

929521QX Alignment Marker 15 bp/1 kb (1.5 ml)CNY

9012598Disposable Filter-Tips, 1100µl (960)Conducting disposable filter-tipsCNY

206152QIAGEN Multiplex PCR Plus Kit (100)CNY

291115mericon RR Soy Kit (96)CNY

291065mericon GMO Screen bar (96)CNY

9001933DML 3000 120-240V QCNY