齐一生物试剂盒组分经过重重优化，每个组分的稳定性都完美的经过高温破坏测验，程度的避免了长途运输和保存过程中抗原抗体的失活.齐一生物试剂盒凭借其完美的标曲.严格的质控.稳定高、易保存，操作简便，已广泛应用在全国免疫学检验研究所的各领域中.深受研究者们的青睐. 齐一生物销售：021－6034 8496；181214 53965；173021 04490

【人补体C3转化酶(C3c)ELISA试剂盒】齐一生物试剂盒凭借其完美的标曲、严格的质控、较高的稳定性，在世界范围内的科研试剂领域中脱颖而出深受研究者们的青睐. 齐一生物销售：021－6034 8496；181214 53965；173021 04490

【友情提示】：本产品仅供科研研究使用，不得用于人体临床直接检测。避免给您带来不必要的损失，请仔细阅读购买说明！

试验所需自备物品:

1. 酶标仪(450nm 波长滤光片)

2. 高精度移液器，EP 管及一次性吸头

3. 37℃恒温箱，双蒸水或去离子水

4. 吸水纸

【人补体C3转化酶(C3c)ELISA试剂盒】试剂盒安全性提示：

1.工作人员实验中避免直接接触终止液和底物A、B。一旦接触到这些液体，请尽快用水冲洗。

2.实验中不要吃喝、抽烟或使用化妆品。

3.不要用嘴吸取试剂盒里的任何成份。

操作注意事项：

1.试剂盒应按标签说明书储存，使用前恢复到室温30分钟后方可使用。稀稀过后的标准品应丢弃，不可保存。

2.实验中不用的板条应立即放回包装袋中，密封保存，以免变质。

3.不用的其它试剂应包装好或盖好。不同批号的试剂不要混用.请在试剂盒保质前使用。

4.使用一次性的吸头以免交叉污染，吸取终止液和底物A、B液时，避免使用带金属部分的加样器。

5.使用干净的塑料容器配置洗涤液。使用前充分混匀试剂盒里的各种成份及样品。

6.洗涤酶标板时应充分拍干，不要将吸水纸直接放入酶标反应孔中吸水。

7.底物A应挥发，避免长时间打开盖子。底物B对光敏感，避免长时间暴露于光下。避免用手接触，有毒。实验完成后应立即读取OD值。

8.加入试剂的顺序应一致，以保证所有反应板孔温育的时间一样。

9.按照说明书中标明的时间、加液的量及顺序进行温育操作。

【人补体C3转化酶(C3c)ELISA试剂盒】齐一生物供应ELISA试剂盒检测的目的是为实验提供准确可靠的定量分析实验依据。为了保证实验数据的可靠性，在实验过程中必须坚持全面质量控制和全过程质量控制。在收集标本前都必须有一个完整的计划。

操作步骤
实验开始前，请提前配置好所有试剂，试剂或样品稀释时，均需混匀，混匀时尽量避免起泡。每次检测都应该做标准曲线。如样品浓度过高时，用样品稀释液进行稀释，以使样品符合试剂盒的检测范围。
1.         加样：分别设空白孔、标准孔、待测样品孔。空白孔加样品稀释液100μl，余孔分别加标准品或待测样品100μl，注意不要有气泡，加样将样品加于酶标板孔底部，尽量不触及孔壁，轻轻晃动混匀，酶标板加上盖或覆膜，37℃反应120分钟。
为保证实验结果有效性，每次实验请使用新的标准品溶液。
2.         弃去液体，甩干，不用洗涤。每孔加生物素标记抗体工作液 100μl（取1μl生物素标记抗体加99μl生物素标记抗体稀释液的比例配制，轻轻混匀，在使用前一小时内配制），37℃,60分钟。
3.         温育60分钟后，弃去孔内液体，甩干，洗板3次，每次浸泡1-2分钟，350μl/每孔，甩干。
4.         每孔加辣根过氧化物酶标记亲和素工作液（同生物素标记抗体工作液） 100μl，37℃，60分钟。
5.         温育60分钟后，弃去孔内液体，甩干，洗板5次，每次浸泡1-2分钟，350μl/每孔，甩干。
6.         依序每孔加底物溶液90μl，37℃避光显色（30分钟内，此时肉眼可见标准品的前3-4孔有明显的梯度蓝色，后3-4孔梯度不明显，即可终止）。
7.         依序每孔加终止溶液50μl，终止反应（此时蓝色立转黄色）。终止液的加入顺序应尽量与底物液的加入顺序相同。为了保证实验结果的准确性，底物反应时间到后应尽快加入终止液。
8.         用酶联仪在450nm波长依序测量各孔的光密度（OD值）。 在加终止液后15分钟以内进行检测。
【人补体C3转化酶(C3c)ELISA试剂盒】注：
1. 用户在初次使用试剂盒时，应将各种试剂管离心数分钟，以便试剂集中到管底。
2. 每次实验留一孔作为空白调零孔，该孔不加任何试剂，只是*后加底物溶液及2N H2SO4。测量时先用此孔调OD值至零。
3. 为防止样品蒸发，试验时将反应板放于铺有湿布的密闭盒内，酶标板加上盖或覆膜。
4. 未使用完的酶标板或者试剂，请于2-8℃保存。标准品、生物素标记抗体工作液、辣根过氧化物酶标记亲和素工作液请依据所需的量配置使用。请勿重复使用已稀释过的标准品、生物素标记抗体工作液或、辣根过氧化物酶标记亲和素工作液。
5. 建议检测样品时均设双孔测定，以保证检测结果的准确性。
以上资料仅供您参考，如有问题欢迎来电，齐一生物销售：021－6034 8496；181214 53965；173021 04490

9012716Troughs, 25ml (100 troughs + lids)CNY

133230NeXtalStock Sodium cacodyl. pH 6.6 (100)CNY

9002128QIAsymphony RGQ, PrioPLUS"QIAsymphony RGQ Instruments

Installation of QIAsymphony RGQ   instrument & Introductory training 

3 years coverage on parts, labor ａnd travel

On site service, 48 hr response

RGQ, Regional Repair Center service, turn around time 7-10 days

1 Preventive Maintenance service for each year of coverage

Priority service provision over non-contract customers

Documented installation & PM report

Travel, parts, labor ａnd shippment included"CNY

9002048Rotor-Gene Q MDx 6plex, PriorityRotor-Gene Q MDx 6plex, Priority includes the Rotor-Gene Q system, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with on site introductory training. Priority package extends warranty period of the system to 2 years with shipment to Repair Center Service has response turnaround time (repair) 7-10 days. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Replacement system (Loaner) provision included (provision time 2-3 days) with transportation cost coverage. The service package includes all parts, labor ａnd travel costs with installation performance ａnd service visits documented on a GMP/GLP-compliant Field Service documents.CNY

9002043Rotor-Gene Q MDx 6plex SystemCNY

56504QIAamp DNA Investigator Kit (50)CNY

929580QX RNA Size Marker (20µl),KGCNY

929564QX Pro Marker 14-97 kDa (575 µg)CNY

972422PyroMark Q96 SNP APOE (96)For detection of two polymorphism in APOE (APOE 112 ａnd APOE 158)CNY

133224NeXtalStock Sodium ac. pH 4.4 (200)CNY

301425Effectene Transfection Reagent (1 ml)1 ml Effectene Reagent, Enhancer, Buffer; for 40 transfections in 60 mm dishes or 160 transfections in 12-well platesCNY

9012693Probe Tubing Holding Screw with bushingCNY

9002008Rotor-Gene Q MDx 2plex, PriorityRotor-Gene Q MDx 2plex, Priority includes the Rotor-Gene Q system, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with on site introductory training. Priority package extends warranty period of the system to 2 years with shipment to Repair Center Service has response turnaround time (repair) 7-10 days. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Replacement system (Loaner) provision included (provision time 2-3 days) with transportation cost coverage. The service package includes all parts, labor ａnd travel costs with installation performance ａnd service visits documented on a GMP/GLP-compliant Field Service documents.CNY

971460therascreen® KRAS Pyro Kit (24) CECNY

292043mericon Turkey (24)CNY

133207NeXtalStock K/Na tartrate (200)CNY

292073mericon Ruminant Kit (24)CNY

929104QIAxcel RNA QC Kit v2.0 (1200)CNY

206042Type-it Fast SNP Probe PCR Kit (100)"Features

 Outstanding separation for reliably high call rates

 For difficult SNP loci ａnd for low amounts of template

 Validated using TaqMan SNP Genotyping Assays 

 Up to 40% time savings due to fast-cycling procedure

 Convenient ｍaster mix format ａnd optimized protocol"CNY

205314QuantiTect Reverse Transcription (400)CNY

205313QuantiTect Rev.Transcription Kit (200)"For 200 x 20 µl reactions: gDNA Wipeout Buffer, Quantiscript Reverse Transcriptase, Quantiscript RT Buffer, RT Primer Mix, RNase-Free Water. 

The contents of this kit are 4 x the contents of the 205311 (NO bulk!).

Suitable only for cDNA synthesis followed by real-time analyses."CNY

291055mericon GMO Screen CTP2-CP4EPSPS (96)CNY

9001918PyroMark Q96 MD, PrioPLUS"PyroMark Q96 MD Instrument 

Installation of PyroMark Q96 MD instrument & Introductory training 

Warranty extension to 3 yrs

On site service, 48 hr response

Preventive Maintenance (1 onsite PM per year)

Priority service provision over non-contract customers

Documented installation & PM report

Unlimited number of repair service

All cost covered (travel, labor, parts)"CNY

9001914PyroMark Q24, PrioPLUS"PyroMark Q24 Instrument 

Installation of PyroMark Q24 & Introductory training 

Warranty extension to 3 yrs

Repair Center service, response 7-10

Loaner available in 2 days*

Annual Inspections (1 onsite or RRC  PM per year)

Priority service provision over non-contract customers

Documented installation & PM report

Unlimited number of repair service

All cost covered (travel, labor, parts)

(In the event of shipping logistic limitation, QIAGEN reserves the option to repair the instrument on-site. Response time 48 Hrs)"CNY

9001898QIAxtractor, UV/HEPA (w/o PC) PrioPLUS"QIAxtractor  Instrument 

Installation of QIAxtractor instrument & Introductory training 

Warranty extension to 3 yrs

On site service, 48 hr response

Preventive Maintenance (1 onsite pipetting head service ａnd inspection per year)

Priority service provision over non-contract customers

Documented installation & PM report

Unlimited number of repair service

All cost covered (travel, labor, parts)"CNY

133112NeXtalStock Sodium formate (200)CNY

965152QIAamp DNA Blood BioRobot MDx Kit (12)For 12 x 96 DNA preps: 12 QIAamp 96 Plates,Buffers,† QIAGEN Protease, ElutionMicrotubes CL, Caps, S-Blocks, Tape PadCNY

962241QIAprep 96 Plus BioRobot Kit (4)CNY

51204FlexiGene DNA Kit (50)For purification of DNA from 50 ml whole blood: Buffers, QIAGEN ProteaseCNY

9015484Reagent Holder, 6-bottle, 30 mlCNY