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人CD160分子(CD160)ELISA试剂盒

点击次数:16发布时间:2016/6/14 20:06:20

人CD160分子(CD160)ELISA试剂盒

更新日期:2016/6/14 20:06:20

所 在 地:中国大陆

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简单介绍:"人CD160分子(CD160)ELISA试剂盒 品牌QIYBO 货号QY-H5006 规格:96次/48次适用Homo sapiens human 2-8℃"

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 CD160分子(CD160)ELISA试剂盒ELISA 样本的处理

ELISA 检测的目的是为实验提供准确可靠的定量分析实验依据。为了保证实验数据的可靠性,在实验过程中必须坚持全面质量控制和全过程质量控制。在收集标本前都必须有一个完整的计划。齐一生物销售:0216034 8496181214 53965173021 04490

 

注意事项:

   每个标本量收集体积=  100ul × 检测种类,如要做复孔,标本量收集体积=  100ul × 检测种类×2

   对于作某一个具体指标来说,先用一系列稀释度作一批标本测定,得出对实验有意义的一个稀释度(即测定剂量反应曲线),然后用一个*适稀释度测定即可。

   收集标本前必须清楚要检测的成份是否足够稳定。对收集后当天进行检测的标本,储存在4℃备用,如有特殊原因需要周期收集标本,将标本及时分装后放在-20℃ 或-70℃ 条件下保存。避免反复冻融。标本 2 -8 ℃ 可保存48 小时.-20℃ 可保存1个月。 -70 度可保存6个月。部分激素类标本需添加抑肽酶。

   血清标本采集时,应注意避免溶血,红细胞溶解时会释放出具有过氧化物酶活性的物质,以 HRP为标记的 ELISA 测定中,溶血标本可能会增加非特异性显色。

CD160分子(CD160)ELISA试剂盒   为了保证尿液检测结果的准确性,必须正确收集尿液标本和保存。盛尿容器要清洁干燥。使用一次性的容器(如塑料尿杯),避免因用药并清洗不干净而造成的污染,影响检测结果。尿液标本必须新鲜,留取后,应及时检测或保存,以免细菌繁殖。因在室温中久置后(尤其是夏季)尿中磷酸盐等可析出结晶而干扰检测。

    标本宜在新鲜时检测。如有细菌污染,菌体中可能含有内源性 HRP,也会产生假阳性反应。保存过久可发生聚合在 ELISA中可使本底加深。

   冻结标本融解后,蛋白质局部浓缩,分布不均,应充分混匀宜轻缓,避免气泡,可上下颠倒混和,不要在混匀器上强烈振荡。

   混浊或有沉淀的标本应先离心或过滤,澄清后再检测。

   反复冻融会使蛋白效价降低,所以待测标本如需保存作多次检测,宜少量分装冰存。也可加入适当防腐剂。

   如果您在实验中遇到任何特殊问题,敬请咨询 021-60348496标本类型

ELISA常见的标本一般包括:

   液体类标本:包括血清、血浆、尿液、胸腹水、脑脊液、细胞培养上清等。

   培养细胞

   组织标本

这些标本收集的时间、方法和保存都有一定的要求。

CD160分子(CD160)ELISA试剂盒标本的保存

一般说来,在 5 天内测定的标本可放置于 4℃,超过一周测定的需低温冻存。

对收集后当天进行检测的标本,储存在  4 ℃ 备用,如有特殊原因需要周期收集标本, 将标本及时分装后放在  -20 ℃ 或  -70 ℃ 条件下保存。避免反复冻融。 标本  2 -8 ℃ 可保存  48 小时,-20 ℃ 可保存  1 个月。-70 度可保存  6 个月。部分标本也可加入适当防腐剂,激素类标本需添加抑肽酶。标本的处理 可用作 ELISA 测定的标本十分广泛,体液(如血清)、分泌物(唾液)和排泄物(如尿液)、细胞培养上清、细胞、组织等均可作标本以测定其中某种成份。有些标本可直接进行测定,有些则需经预处理。

◆ 血清:  

室温血液自然凝固  10-20 分钟后,离心  20 分钟左右(  2000-3000   / 分)。收集上清。如有沉淀形成,应再次离心。  

◆ 血浆:  

应根据试剂盒的要求选择  EDTA 、柠檬酸钠或肝素作为抗凝剂,加入  10 %(  v/v )抗凝剂 ( 0.1M 柠檬酸钠或  1% heparin   2.0%EDTA.Na2) 混合  10-20 分钟后,离心  20 分钟左右(  2000-3000   / 分)。仔细收集上清如有沉淀形 成,应再次离心。  

CD160分子(CD160)ELISA试剂盒◆ 尿液、胸腹水、脑脊液:  

用无菌管收集。离心  20 分钟左右(  2000-3000   / 分)。仔细收集上清。如有沉淀形成,应再次离心。  

◆ 细胞培养上清:  

检测分泌性的成份时,用无菌管收集。离心  20 分钟左右(  2000-3000   / 分)。仔细收集上清。检测细胞内的 成份时,用  PBS   PH7.2-7.4 )稀释细胞悬液,细胞浓度达到  100 /ml 左右。通过反复冻融,以使细胞破坏并放出细胞内成份。离心  20 分钟左右(  2000-3000 / 分)。仔细收集上清。保存过程中如有沉淀形成,应再次离心。  

◆ 细胞:

检测细胞的成份时,对于贴壁细胞,去除培养液,用PBS、生理盐水或无血清培养液洗一遍。加入适量裂解液。用枪吹打数下,使裂解液和细胞充分接触。通常裂解液接触细胞10 秒后,细胞就会被裂解。对于悬浮细胞,离心收集细胞,用PBS、生理盐水或无血清培养液洗一遍.加入适量裂解液, 用枪吹打把细胞吹散。用手指轻弹以充分裂解细胞。充分裂解后应没有明显的细胞沉淀。如果细胞量较多,必需分装然后再裂解。充分裂解后,10000-14000g离心3-5 分钟,取上

清,即可进行后续操作。

 

CD160分子(CD160)ELISA试剂盒◆ 组织标本:  

切割标本后,称取重量。加入一定量的  PBS或组织蛋白萃取试剂,缓冲液中可加入蛋白酶抑制剂。用手工或匀浆器将标本匀浆充分。离心  20 分钟左右(  2000-3000   / 分)。仔细收集上清置于  -20 度或  - 70 度保存,如有必要,可以将样品浓缩干燥。分装后一份待检测,其余冷冻备用。齐一生物销售:0216034 8496181214 53965173021 04490  

 

QY-x50QGY-7701肝癌细胞

QY-x51BEL-7405肝癌细胞

QY-x52NCI-H460 [H460]大细胞肺癌细胞0 HTB-177

QY-x53NCI-H460 [H460]大细胞肺癌细胞0 HTB-177

QY-x54BEL-7404肝癌细胞

QY-x55BEL-7402肝癌细胞

QY-x56QG-56肺扁平上皮癌细胞

QY-x57SPC-A-1肺腺癌细胞

QY-x58HGC-27胃癌细胞未分化

QY-x59LTEP-a-2肺腺癌细胞

QY-x60AGS胃腺癌细胞 CRL-1739

QY-x61MGC80-3胃癌细胞

QY-x6295-D高转移肺癌细胞

QY-x63BGC-823胃腺癌细胞低分化

QY-x64BT549乳腺管癌细胞 HTB-122

QY-x65SGC-7901胃腺癌细胞

QY-x66T-47D乳腺管癌细胞 HTB-133

QY-x67T-47D乳腺管癌细胞 HTB-133

QY-x68TE-11食管癌细胞

QY-x69SK-BR-3乳腺腺癌细胞 HTB-30

QY-x70SK-BR-3乳腺腺癌细胞 HTB-30

QY-x71TE-10食管癌细胞

QY-x72ZR-75-30乳腺癌细胞 CRL-1504

QY-x73TE-1食管癌细胞

QY-x74Bcap-37乳腺癌细胞

QY-x75Eca-109食管癌细胞

QY-x76MDA-MB-453乳腺癌细胞 HTB-131

QY-x77CNE鼻咽癌细胞

QY-x78JAR胎盘绒毛癌细胞 HTB-144

 

QY-x79HEp-2喉表皮样癌细胞 CCL-23


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