【友情提示】：本产品仅供科研研究使用，不得用于人体临床直接检测。避免给您带来不必要的损失，请仔细阅读购买说明！

本试剂盒仅供研究使用，齐一生物销售：021－6034 8496；181214 53965；173021 04490欢迎选购！

操作步骤

1.准备试剂，样品和标准品

2.加入准备好的样品和标准品，37℃反应30分钟

3.洗板5次，加入酶标试剂，37℃反应30分钟

4.洗板5次，加入显色液A、B，37℃显色15分钟

5.加入终止液

6.15分钟之内读OD值

7.计算
齐一生物供应ELISA试剂盒检测的目的是为实验提供准确可靠的定量分析实验依据。为了保证实验数据的可靠性，在实验过程中必须坚持全面质量控制和全过程质量控制。在收集标本前都必须有一个完整的计划。

【人细胞色素P450c21B/21-羟化酶(CYP21B)ELISA试剂盒】样本保存条件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6个月

3.-50℃以下的冰箱，可以放一年

【人细胞色素P450c21B/21-羟化酶(CYP21B)ELISA试剂盒】样本收集

1.血清样本用干燥管（红色）或者促凝管（黄色）收集

2.血浆用抗凝管，一般有EDTA抗凝（紫色）、枸橼酸钠抗凝（黑色或者蓝色）

3.动物组织匀浆（细胞）：

              1g组织加入9ml PBS（浓度为0.1M，pH为7.4的）测分泌型指标

              1g组织加入1ml组织裂解液加入8ml PBS 测细胞内

粪便样本：1g样本+9ml PBS（浓度为0.01M，pH为7.4的）

没有PBS的话也可以用一下生理盐水，还是用PBS

【人细胞色素P450c21B/21-羟化酶(CYP21B)ELISA试剂盒】样本离心转速及时间

1.试管采集的样本：3000转，离心五分钟取上清

2.离心管或者EP管采集的样本：5000－－8000转3分钟取上清

3..样本保存－－如果样品不立即使用，应将其分成小部分-70 ℃保存，避免反复冷冻。尽可能的不要使用溶血或高血脂血。如果血清中大量颗粒，检测前先离心或过滤。不要在37℃或更高的温度加热解冻。应在室温下解冻并确保样品均匀地充分解冻。

【人细胞色素P450c21B/21-羟化酶(CYP21B)ELISA试剂盒】以上样本采集及处理方式供您参考，如有问题请来电，齐一生物销售：021－6034 8496；181214 53965；173021 04490

QY-x1444       大鼠肝星形细胞CFSC-2G                                     

QY-x1445       大鼠肝星形细胞CFSC-3H & 5H                                   

QY-x1446       大鼠肝上皮样干细胞WB-F344                                     

QY-x1447       鼠肌原细胞C2C12                                         

QY-x1448       大鼠肺上皮Ⅱ型细胞RLE-6TN                                     

QY-x1449       小鼠肺成纤维细胞L929                                        

QY-x1450       大鼠胸主动脉平滑肌细胞，ATCC：A10VSMC                                   

QY-x1451       小鼠胚成纤维包装细胞PA317                                      

QY-x1452       小鼠胚成纤维包装细胞ψ2                                    

QY-x1453       叙利亚仓鼠肾细胞BHK-21                                    

QY-x1454       BHK                                   

QY-x1455       转S9基因仓鼠卵巢细胞ZA1                                        

QY-x1456       转S9基因仓鼠卵巢细胞ZA6                                        

QY-x1457       大鼠巨噬细胞MC                                   

QY-x1458       非洲绿猴肾细胞COS-1                                          【人细胞色素P450c21B/21-羟化酶(CYP21B)ELISA试剂盒】

QY-x1459       非洲绿猴肾细胞COS-6                                         

QY-x1460       猴肾细胞系BSC-1                                   

QY-x1461       小鼠肾小球系膜细胞MES-13                                       

QY-x1462       小鼠肾系膜细胞MC53                                   

QY-x1463       大鼠肾成纤微细胞NRK-49F                                         

QY-x1464       小鼠皮肤细胞JB6-C30                                   

QY-x1465       小鼠皮肤细胞JB6-C41                                   

QY-x1466       小鼠前成骨细胞MC-3T3                                       

QY-x1467       小鼠成骨细胞系MC3T3-E1                                   

QY-x1468       鼠胚成骨细胞3T3-E1                                     

QY-x1469       大鼠肾小管上皮细胞，ATCC：CRL-6509NRK                                   

QY-x1470       大鼠肾细胞NRK-52E                                     

QY-x1471       小鼠饲养层上皮细胞HPT-8                                   

QY-x1472       LLC:Leuwis肺癌细胞                                     

QY-x1473       化学转化小鼠鼻咽上皮细胞TMNE                                      

9237329  Upgrade, BR & 3 ext. devices w. sched.      Upgrade BioRobot with three approved integration devices using scheduling software  CNY                            【人细胞色素P450c21B/21-羟化酶(CYP21B)ELISA试剂盒】

9237168  Bottle Sheath Supply             CNY                           

9233004  Fan 24V, for BR3000 with VariTerm            CNY                           

9237151  Power Supply        CNY                           

9237123  Upgrade App., QIAquick 96 PCR  On-site application Upgrade for standard QIAGEN QIAquick 96 including kit   CNY                           

9237106  Protocol Dev./Opt., on-site (2-days)     The QIAGEN BioRobot Systems Protocol Development/Optimization On-Site Visit includes all travel ａnd labor charges for a site visit up to 2 days by a QIAGEN Instrumentation Service Specialist to write and/or opimize protocols for the BioRobot System. Parts, consumable chemistries, ａnd accessories not included. Custom protocols are the sole responsibility of the customer ａnd neither warrantied nor guaranteed. CNY                           

9237097  Training, BR QIAGEN Advanced  A minimum of 4 people are required to attend. The QIAGEN BioRobot Systems Advanced Training includes three days of  training at our training facility to introduce users to advanced software topics for the  QIAGEN BioRobot Systems.  Designed for users with a basic understanding of the use ａnd programming of QIAGEN BioRobot systems who want to write complex protocols for their instrument, the advanced training starts with a brief review of basic concepts such as teaching slots ａnd racks before exploring the higher-level programming features of the QIAsoft Operating System.  Topics include the structure ａnd use of functions, using variable for programming flexibility, IF/ELSE/END boolean logic commands, ａnd parameterized pipetting using tables.  Users will write, test ａnd run protocols they have developed to demonstrate ａnd reinforce training success.    CNY                           

9236422  Power supply, replacement kit, TWI            CNY                           

9232968  Print YZ96-Adapter-Board Plato-7        CNY                           

9236363  Reservoir XYP              CNY                           

9236353  Tubing Sample Needle to 3-Way Valve         CNY                           

9236329  2-Way Valve (Sheath or vent)        CNY                           

9236160  Potentionmeter, rotary, kit, RapidPlate          CNY                           

9236154  Online Fill Pump RP             CNY                           

9236126  QIAGEN Multiwell-Plate Holder , silver       Holder for accommodating multiwell plates on the Microplate station of the BioRobot MDx worktable      CNY                           

9236120  Runner, Drawer, BR8 RD             CNY                           

9235790  Ramp, Locking, Drawer, BR8 RD        CNY                            【人细胞色素P450c21B/21-羟化酶(CYP21B)ELISA试剂盒】

9230745  Z-Lead screw for Plato 7             CNY                           

9235690  Cable X-Main, RCS1            CNY                           

9235572  Shaker Tool, RCS1        CNY                           

9230314  Cable, RS232, syringe pump, 225mm          CNY                           

9230283  Cable, Interface, X-main to Washer             CNY                           

5000-1302      Capacitor 2UF, SKR1            CNY                           

5000-1284      DML 2000 - Power Cord, 220V D Type (GB)            CNY                           

5000-1254      Power Supply PCB, DML2           CNY                           

5000-1250      CPU DML 2000 ASSRY Kit, DML2             CNY                           

6000-3401      RCS Upgrade Package          CNY                           

6000-3116      Plate Washer I - 105 Micron Filters             CNY                           

6000-00183    Plate Washer - Manifold        CNY                           

6000-00177    Plate Washer - Syringe Assembly         CNY                           

5000-1501      Holder Fuse, HTR1        CNY                           

5135-1220      hc2 CT-ID DNA Test            CNY