ELISA 检测的目的是为实验提供准确可靠的定量分析实验依据。为了保证实验数据的可靠性，在实验过程中必须坚持全面质量控制和全过程质量控制。在收集标本前都必须有一个完整的计划。齐一生物销售：021－6034 8496；181214 53965；173021 04490

注意事项：

◆   每个标本量收集体积＝  100ul × 检测种类，如要做复孔，标本量收集体积＝  100ul × 检测种类×2 。

◆   对于作某一个具体指标来说，先用一系列稀释度作一批标本测定，得出对实验有意义的一个稀释度（即测定剂量反应曲线），然后用一个*适稀释度测定即可。

◆   收集标本前必须清楚要检测的成份是否足够稳定。对收集后当天进行检测的标本，储存在4℃备用，如有特殊原因需要周期收集标本，将标本及时分装后放在-20℃ 或-70℃ 条件下保存。避免反复冻融。标本 2 -8 ℃ 可保存48 小时.-20℃ 可保存1个月。 -70 度可保存6个月。部分激素类标本需添加抑肽酶。

◆   血清标本采集时，应注意避免溶血，红细胞溶解时会释放出具有过氧化物酶活性的物质，以 HRP为标记的 ELISA 测定中，溶血标本可能会增加非特异性显色。

【人弹性蛋白酶(Elastase)ELISA试剂盒】◆   为了保证尿液检测结果的准确性，必须正确收集尿液标本和保存。盛尿容器要清洁干燥。使用一次性的容器（如塑料尿杯），避免因用药并清洗不干净而造成的污染，影响检测结果。尿液标本必须新鲜，留取后，应及时检测或保存，以免细菌繁殖。因在室温中久置后（尤其是夏季）尿中磷酸盐等可析出结晶而干扰检测。

◆    标本宜在新鲜时检测。如有细菌污染，菌体中可能含有内源性 HRP，也会产生假阳性反应。保存过久可发生聚合在 ELISA中可使本底加深。

◆   冻结标本融解后，蛋白质局部浓缩，分布不均，应充分混匀宜轻缓，避免气泡，可上下颠倒混和，不要在混匀器上强烈振荡。

◆   混浊或有沉淀的标本应先离心或过滤，澄清后再检测。

◆   反复冻融会使蛋白效价降低，所以待测标本如需保存作多次检测，宜少量分装冰存。也可加入适当防腐剂。

◆   如果您在实验中遇到任何特殊问题，敬请咨询 021-60348496标本类型

ELISA常见的标本一般包括：

◆   液体类标本：包括血清、血浆、尿液、胸腹水、脑脊液、细胞培养上清等。

◆   培养细胞

◆   组织标本

这些标本收集的时间、方法和保存都有一定的要求。

【人弹性蛋白酶(Elastase)ELISA试剂盒】标本的保存

一般说来，在 5 天内测定的标本可放置于 4℃，超过一周测定的需低温冻存。

对收集后当天进行检测的标本，储存在  4 ℃ 备用，如有特殊原因需要周期收集标本， 将标本及时分装后放在  -20 ℃ 或  -70 ℃ 条件下保存。避免反复冻融。 标本  2 -8 ℃ 可保存  48 小时，-20 ℃ 可保存  1 个月。-70 度可保存  6 个月。部分标本也可加入适当防腐剂，激素类标本需添加抑肽酶。标本的处理 可用作 ELISA 测定的标本十分广泛，体液（如血清）、分泌物（唾液）和排泄物（如尿液）、细胞培养上清、细胞、组织等均可作标本以测定其中某种成份。有些标本可直接进行测定，有些则需经预处理。

◆ 血清：  

室温血液自然凝固  10-20 分钟后，离心  20 分钟左右（  2000-3000 转  / 分）。收集上清。如有沉淀形成，应再次离心。  

◆ 血浆：  

应根据试剂盒的要求选择  EDTA 、柠檬酸钠或肝素作为抗凝剂，加入  10 ％（  v/v ）抗凝剂 ( 0.1M 柠檬酸钠或  1% heparin 或  2.0%EDTA.Na2) 混合  10-20 分钟后，离心  20 分钟左右（  2000-3000 转  / 分）。仔细收集上清如有沉淀形 成，应再次离心。  

【人弹性蛋白酶(Elastase)ELISA试剂盒】◆ 尿液、胸腹水、脑脊液：  

用无菌管收集。离心  20 分钟左右（  2000-3000 转  / 分）。仔细收集上清。如有沉淀形成，应再次离心。  

◆ 细胞培养上清：  

检测分泌性的成份时，用无菌管收集。离心  20 分钟左右（  2000-3000 转  / 分）。仔细收集上清。检测细胞内的 成份时，用  PBS （  PH7.2-7.4 ）稀释细胞悬液，细胞浓度达到  100 万 /ml 左右。通过反复冻融，以使细胞破坏并放出细胞内成份。离心  20 分钟左右（  2000-3000 转 / 分）。仔细收集上清。保存过程中如有沉淀形成，应再次离心。  

◆ 细胞：

检测细胞的成份时，对于贴壁细胞，去除培养液，用PBS、生理盐水或无血清培养液洗一遍。加入适量裂解液。用枪吹打数下，使裂解液和细胞充分接触。通常裂解液接触细胞10 秒后，细胞就会被裂解。对于悬浮细胞，离心收集细胞，用PBS、生理盐水或无血清培养液洗一遍.加入适量裂解液, 用枪吹打把细胞吹散。用手指轻弹以充分裂解细胞。充分裂解后应没有明显的细胞沉淀。如果细胞量较多，必需分装然后再裂解。充分裂解后，10000-14000g离心3-5 分钟，取上

清，即可进行后续操作。

【人弹性蛋白酶(Elastase)ELISA试剂盒】◆ 组织标本：  

切割标本后，称取重量。加入一定量的  PBS或组织蛋白萃取试剂，缓冲液中可加入蛋白酶抑制剂。用手工或匀浆器将标本匀浆充分。离心  20 分钟左右（  2000-3000 转  / 分）。仔细收集上清置于  -20 度或  - 70 度保存，如有必要，可以将样品浓缩干燥。分装后一份待检测，其余冷冻备用。齐一生物销售：021－6034 8496；181214 53965；173021 04490  

QY-x2014       T47D 乳腺导管癌 1640+15%胎牛血清                                      

QY-x2015       ZR-75-1 乳腺导管癌 1640+10%胎牛血清                                   

QY-x2016       A875 黑色素瘤 1640+10%胎牛血清                                    

QY-x2017       SK-HEL-1 皮肤黑色素瘤                                       

QY-x2018       A431 皮肤基底细胞癌                                    【人弹性蛋白酶(Elastase)ELISA试剂盒】

QY-x2019       Jurkat 急性T淋巴细胞白血病 1640+10%胎牛血清                                         

QY-x2020       U937 单核细胞白血病 1640+10%胎牛血清                                       

QY-x2021       MJ 淋巴瘤 1640+10%胎牛血清                                   

QY-x2022       CEM 白血病 1640+10%胎牛血清                                       

QY-x2023       K562 慢性髓原白血病 1640+10%胎牛血清                                       

QY-x2024       HPB-ALL T细胞白血病 1640+10%胎牛血清                                      

QY-x2025       HL-60 白血病 1640+10%胎牛血清                                      

QY-x2026       Daudi B淋巴细胞瘤 1640+10%胎牛血清                                    

QY-x2027       SK-BR-3 乳腺腺癌 1640+15%胎牛血清                                     

QY-x2028       MCF-7 乳腺腺癌 1640+10%胎牛血清                                        

QY-x2029       MCF-7/Adr 乳腺腺癌/阿霉素耐药株 1640+10%胎牛血清                                        

QY-x2030       A2780 卵巢癌 1640+10%胎牛血清                                     

QY-x2031       CoC1 卵巢癌 1640+10%胎牛血清                                       

QY-x2032       CoC2 卵巢癌 1640+10%胎牛血清                                       

QY-x2033       OVCAR-3 卵巢癌 DMEM+10%胎牛血清                                    

QY-x2034       SK-OV-3 卵巢腺癌 1640+10%胎牛血清                                      【人弹性蛋白酶(Elastase)ELISA试剂盒】

QY-x2035       SK-OV-3/DDP 卵巢腺癌顺铂耐药株 1640+10%胎牛血清                                        

QY-x2036       HeLa 宫颈癌 1640+10%胎牛血清                                       

QY-x2037       HeLaS3 宫颈癌 1640+10%胎牛血清                                    

QY-x2038       HEC-1B 子宫内膜癌                                      

QY-x2039       JEG-3 绒癌 1640+10%胎牛血清                                         

QY-x2040       Du145 前列腺癌 高糖DMEM+10%胎牛血清                                    

QY-x2041       LNCaP 前列腺癌                                    

QY-x2042       LNCaP clone 前列腺癌                                   

QY-x2043       PC-3 前列腺癌 DMEM+10%胎牛血清                                       

9238672  Buffer 40/28          CNY                            【人弹性蛋白酶(Elastase)ELISA试剂盒】

9238654  Power board cable, 4-channel              CNY                           

9238634  Rotor identification        CNY                           

9238623  Relay 16 A             CNY                           

9241820  Pyro Q96 MD, Basic Agreement   "Repair service delivery: onsite

Service response time (repair): 5 business days

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) provision: not included

Transportation cost coverage: not applicable"      CNY                           

9241170  Transport lock, QX              CNY                           

9240977  Tray, sample tracking, Qsym        CNY                           

9240966  Sensor, z, pipetting system, Qsym        CNY                           

9240686  Autodrain, Kit, Water Trap, AP196/98          CNY                           

9240681  Dump Tray, Overflow Control, AP96/196           CNY                           

9240787  Update, Capper In/Out Motor, AP98            CNY                           

6000-5013R    LumiCheckPlate User Package(Refurbished)              CNY                           

9241783  Rotor - Gene Q Demopool Box            CNY                           

9241769  QIAxtractor, Full Agreement  The Full Agreement, QIAxtractor provides full service coverage for the QIAxtractor ａnd the CAS 1280. Costs for service visits are fully covered including travel, labor, ａnd parts. One on-site pipetting head service ａnd inspection is included . With a Full Agreement, there is a priority response time of 48 hours (2 working days).       CNY                           

9241368  Interlock, IL1, EZ1a             CNY                           

9241366  Cable, UV lamp voltage converter, EZ1a              CNY                           

9240762  Co-Processor, AP96             CNY                            【人弹性蛋白酶(Elastase)ELISA试剂盒】

9240760  Update, Bucket/Elevator Head, AP196/98            CNY                           

9243549  Pyro Q96 MD, PM Service   "Service response time (repair): not applicable

Periodic inspection/maintenance: yes

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts : not included

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage" CNY                           

9242786  RCS, Full Agreement, 2PM    "Repair service delivery: onsite ａnd to Regional Repair Center

Service response time (repair): 48 hours/2 business days or 7-10 working days for RRC repairs

Periodic inspection/maintenance: yes, 2/agreement year

Inspection/maintenance delivery: onsite for RCS only

Cost coverage for Repair Parts : yes, full coverage

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, included"       CNY                           

9242714  Validation Support, 5d, QIAgility   The QIAGEN QIAgility Accelerated Validation Support service includes all travel ａnd labor charges for a site visit up to 5 days by a QIAGEN Specialist.  A QIAGEN specialist will provide an introduction to the QIAgility system ａnd software, review the User Manual, as well as hands-on user support ａnd QIAgility instrument operation.  The Accelerated Validation service includes guidance on the laboratory’s validation experimental design, internal validation documentation support, ａnd QIAGEN sponsored data templates. The QIAGEN Specialist will support the customer by running samples through the QIAGEN-only extraction method/system (does not include phenol-chloroform or similar methods), as well as general wet lab support such as pipetting for quantification, STR ａnd CE setup, normalization, etc. to produce the data needed for the validation study. The customer is responsible for submitting the data to QIAGEN for assisted data analysis. The customer is responsible for final documentation ａnd report using QIAGEN prepared templates provided with the final product. Enough consumable chemistry ａnd accessories (QIAGEN or other vendors) should be purchased to cover the validation experimental design ａnd are not included in the cost of validation support.  Please indicate which processes (quant setup, normalization, PCR setup, CE setup) ａnd kit manufacturer (Applied Biosystems, Promega, QIAGEN) need to be included in the validation.   CNY                           

9242531  QIAsymphony SPAS, PM Agreement          CNY                           

9242546  Probe Holder Assembly, LX200, LC            CNY                           

9242152  Upgrade, CAS1820 vacuum DX/VX     "The Upgrade, CAS 1820 vacuum DX/VX upgrades the X-tractorgene to use the improved vacuum system for the QIAxtractor. The upgrade is conducted as an on-site service, performed by a QIAGEN service specialist.

The upgrade allows for the usage of the QIAGEN DX ａnd VX capture plates ａnd the disposable silicon mats.

During the service, the new vacuum system is installed ａnd the software is updated. Please note that due to the software upgrade the instrument will only run the QIAxtractor software applications. Customized run files from the previous version will have to be adapted beforehand for additional fees."     CNY                           

5000-1404      Plate Cartridge 110V, VTX2          CNY                           

5000-1277      Top Housing W LID, DML2         CNY                           

5050-1029      Mouse, PC System spare             CNY                           

5025-1011      Expand 4 Pipettor 240V        CNY                           

9240747  Belt, Reagent Pump 117 groove, AP             CNY                           

9240720  Cable Carrier, Tubing, Dispense Head, AP           CNY                           

9240700  Tubing, Waste, 1 1\4", AP            CNY                           

9243690  DML Luminometer, Repair Fee     DML Repair Fee covers Regional Repair Center service for non-service agreement covered DML 2000 ａnd DML 3000 units. Includes labor, parts ａnd shipping materials.     CNY