ELISA酶联法试剂盒操作因固相载体的形成不同而有所差异，国内医学检验一般均用板式点。本文将叙述板式ELISA各个操作步骤的注意要点，珠式、管式及磁性球ELSIA，国外试剂均与特殊仪器配合应用，两者均有详细的使用说明，严格遵照规定操作，必能得出准确的结果。齐一生物销售：021－6034 8496；181214 53965；173021 04490

首先齐一生物优质的试剂盒，良好的仪器和正确的操作是保证ELISA检测结果准确可靠必要条件。所以ELISA试剂盒的购买，试剂质量的保证以及实验前实验仪器的检察都是很有重要性的。保证实验材料的质量之后要严格按照实验步骤进行。齐一生物销售：021－6034 8496；181214 53965；173021 04490

【山羊生长激素释放多肽(GHRP)ELISA试剂盒现货】齐一生物ELISA试剂盒

1.精心挑选的进口抗体、标准品、显色剂和酶标板全部为进口产品实现*佳性能；

2.原材料批量进口加上自配的特殊配方稀释剂保证高品质产品性能的同时兼顾成本；

3.采用技术，严格按照标准生产方案组装生产；

4.预包被微孔板的批内和批间变异系数均小于10 ％；

5.经过严格交叉反应和干扰测试及稳定性试验；

6.明确的敏感度；

7.针对所有样品类型达到*佳的性能；

8.广泛的检测范围可以满足大多数检测要求；

9.完善的售后服务和免费的技术支持免去您的后顾之忧；

10.强大的生产能力和充足的储备、操作熟练的技术人员及高效的生产流程，随时可以组装出您定制的产品。

【山羊生长激素释放多肽(GHRP)ELISA试剂盒现货】齐一生物仅供实验人、大鼠、小鼠、兔等动物实验标本检测使用！得用于人体临床直接检测。避免给您带来不必要的损失，请仔细阅读购买说明！齐一生物销售：021－6034 8496；181214 53965；173021 04490

ELISA试剂盒问题描述

标准曲线梯度差：

吸液或加液不准请检查移液器及吸头

标准品稀释不正确溶解标准品稍微旋转瓶身，轻轻混匀使粉末完全溶解

洗涤不完全保证洗涤时间和洗涤次数及每孔的加液量

显色很弱或无色：

孵育时间太短保证充足的孵育时间

实验温度不正确使用推荐的实验温度

试剂体积不够或漏加、稀释不正确检查吸液及加液过程，保证所有试剂按顺序足量添加

读数数值低：

酶标仪设置不正确 在酶标仪上检查波长及滤光片设置

提前打开酶标仪预热：变异系数大加液不正确检查加液情况

【山羊生长激素释放多肽(GHRP)ELISA试剂盒现货】背景值高：

检测抗体的工作浓度使用推荐的稀释倍数

酶标板洗涤不完全重新阅读操作手册，保证清洗完全；如果用自动洗板机，请检查所有的出口是否有堵塞

洗液有污染配制新鲜的洗液

灵敏度低：

ELISA试剂盒保存按说明书要求保存相关试剂

读数前未终止OD读数前应在每孔中加入终止液

齐一生物客户寄标本时需注明以下情况：

1、标本编号；2、所测项目；3、是否做复孔；3、联系方式；4、实验后标本是否寄回。

客户必知：

客户应对所提供的材料及信息负责，如因客户提供的材料及信息不准确而引起的实验延误或经济损失由客户承担。

试剂盒样本要求

在收集标本前都必须有一个完整的计划，必须清楚要检测的成份是否足够稳定。我们提倡新鲜标本尽早检测，对收集后当天就进行检测的标本，及时储存在4℃备用，如有特殊原因需要周期收集标本，请造模取材后，将标本及时分装后放在-20℃或-70℃条件下保存。因冰室与室温存在一定温差，蛋白极易降解，直接影响实验质量，所以避免反复冻融。具体操作注意事项请与我司技术人员沟通。齐一生物销售：021－6034 8496；181214 53965；173021 04490

【山羊生长激素释放多肽(GHRP)ELISA试剂盒现货】液体类标本：标本必须为液体，不含沉淀。包括血清、血浆、尿液、胸腹水、脑脊液、细胞培养上清、组织匀浆等。

1. 血清：室温血液自然凝固10-20分钟后，离心20分钟左右（2000-3000转/分）。仔细收集上清。保存过程中如有沉淀形成，应再次离心。

2. 血浆：应根据标本的要求选择EDTA、柠檬酸钠或肝素作为抗凝剂，混合10-20分钟后，离心20分钟左右（2000-3000转/分）。仔细收集上清。保存过程中如有沉淀形成应再次离心。

3. 尿液、胸腹水、脑脊液：用无菌管收集。离心20分钟左右（2000-3000转/分）。仔细收集上清。如有沉淀形成，应再次离心。

4. 细胞培养上清：检测分泌性的成份时，用无菌管收集。离心20分钟左右（2000-3000转/分）。仔细收集上清。

5. 培养细胞:检测细胞内的成份时，用PBS（PH7.2-7.4）稀释细胞悬液，细胞浓度达到100万/ml左右。通过反复冻融或加入组织蛋白萃取试剂，以使细胞破坏并放出细胞内成份。离心20分钟左右（2000-3000转/分）。仔细收集上清。保存过程中如有沉淀形成，应再次离心。

6. 组织标本：切割标本后，称取重量。加入一定量的PBS，缓冲液中可加入1μg/L蛋白酶抑制剂或50U/ml的Aprotinin（抑肽酶）。用手工或匀浆器将标本匀浆充分。离心20分钟左右（2000-3000转/分）。仔细收集上清置于-20度或-70度保存，如有必要，可以将样品浓缩干燥。分装后一份待检测，其余冷冻备用。

7. 标本采集后尽早进行提取，提取按相关文献进行，提取后应尽快进行实验。若不能马上进行试验，可将标本放于-20℃保存，但应避免反复冻融

8.不能检测含NaN3的样品，因NaN3抑制辣根过氧化物酶的（HRP）活性。

9.如果样品不能立即检测，应将其分装，-70 ℃保存，避免反复冷冻。保存过程中如出现沉淀，应再次离心。血液标本尽可能的不要使用溶血或高血脂血。如果血清中含大量颗粒，检测前先离心或过滤。不要在37℃或更高的温度加热解

冻。应在室温下解冻并确保样品均匀地充分解冻。

QY-x1384       小鼠肺癌细胞LLC                                         

QY-x1385       小鼠胃癌细胞MFC                                        

QY-x1386       小鼠艾氏腹水癌细胞EAC                                     

QY-x1387       小鼠腹水瘤细胞SAC-II B2                                    

QY-x1388       小鼠腹水瘤细胞SAC-II C3                                    

QY-x1389       小鼠乳腺癌高转移细胞MA-891                                    

QY-x1390       小鼠乳腺肿瘤细胞C127                                        

QY-x1391       大鼠乳腺癌细胞SHZ-88                                        

QY-x1392       小鼠骨髓瘤细胞SP2/0                                   

QY-x1393       鼠骨髓瘤细胞SP2/0-Ag14                                     

QY-x1394       小鼠骨髓瘤细胞P3-X63-Ag8.653                                          【山羊生长激素释放多肽(GHRP)ELISA试剂盒现货】

QY-x1395       小鼠骨髓瘤细胞P3/NSI/1-Ag4-1[NS-1]                                       

QY-x1396       小鼠骨髓瘤细胞FO                                       

QY-x1397       小鼠淋巴瘤细胞Yac-1                                    

QY-x1398       小鼠淋巴瘤细胞EL4                                      

QY-x1399       小鼠淋巴瘤细胞EL4.IL-2                                      

QY-x1400       小鼠黑色素瘤细胞B16F1                                      

QY-x1401       鼠黑色素瘤细胞系B16-Fo                                     

QY-x1402       小鼠黑色素瘤高转移细胞B16F10                                        

QY-x1403       小鼠黑色素瘤细胞B16BL6                                    

QY-x1404       小鼠黑色素瘤细胞B16                                   

QY-x1405       小鼠SRSV转化的3T3细胞SRSV/3T3                                       

QY-x1406       小鼠胚胎成纤维细胞BALB/3T3 clone A31                                   

QY-x1407       小鼠肝癌细胞H22                                   

QY-x1408       小鼠肝癌细胞Hepa 1-6                                   

QY-x1409       大鼠肝癌细胞CBRH-7919                                     

QY-x1410       大鼠肝癌细胞RH-35                                       【山羊生长激素释放多肽(GHRP)ELISA试剂盒现货】

QY-x1411       小鼠畸胎瘤细胞F9                                        

QY-x1412       小鼠畸胎瘤细胞P19                                      

QY-x1413       大鼠神经胶质瘤细胞C6                                        

9235295  Sound absorber (muffler)             CNY                           

9235277  Board, HS shaker, BR8000           CNY                           

9230202  Safety shield for size1           CNY                           

9234858  Toubing coupling piece (washer tubing)              CNY                           

9238675  Operating mechanism,  left mech. lock              CNY                           

9238655  Computer board cable, 3-channel         CNY                           

9238633  Grey wedge (long) " This is a thin plastic sheet with a printed gradient of dots that is held by a bracket on the motor so that it is situated in the u shape of the sensor.

SIGMA sensor"      CNY                           

9238622  Main board Zent3, no Eprom (>SN85353)          CNY                           

9241891  QIArent&go - Rent out fee Rotor-Gene              CNY                           

9241823  Pyro Q96 ID, Full Agreement       The Full Cover Agreement, PyM provides full service coverage for the PyroMark instrument. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one on-site inspection service, with costs for labor ａnd parts covered. With a Full Agreement, there is a response time of 2 working days.       CNY                           

9241816  Pyro Q96 MD, PM Agreement             CNY                            【山羊生长激素释放多肽(GHRP)ELISA试剂盒现货】

9241807  Pyro Q96 ID, Installation      Installation ａnd Introductory Training PyroMark is an on-site service, conducted by a QIAGEN Service Specialist. It covers the installation ａnd commissioning of the instrument ａnd the handling of the PyroMark systems hardware. It trains the user on the operation of the PyroMark software ａnd the setup ａnd execution of a pyrosequencing run. After the training the user will be able to prepare samples, set-up ａnd run the instrument, analyse the results ａnd design new assays as well as troubleshoot new assays ａnd perform basic maintenance on the sample preparation tool ａnd instrument. All travel ａnd labor costs ａnd well as material for the demonstration run are included. The service is documented in accordance with GMP/GLP requirements       CNY                           

9241125  Thermal Joint Compound             CNY                           

9240978  PCB, connector, sample tracking, Qsym             CNY                           

9240947  Encoder, x, portal, Qsym             CNY                           

9234821  Manual, user, QS 4.0, US             CNY                           

9240785  Reagent #1 Manifold Update, AP98              CNY                           

9241784  QIAxcel, Training   "The Training, QIAxcel is a 2 days on-site service for up to four QIAxcel users, conducted by a QIAGEN Field Service Specialist. It includes an introduction to operation of the QIAxcel system hardware ａnd the usage of the BioCalculator software. It covers the preparation of sample material, run preparation, data acquisition, ａnd data evaluation. The training is performed using standardized sample material; customer samples may be processed upon request.

All travel ａnd labor costs are included. The service is documented in accordance with GMP/GLP requirements. Training certificates for participants can be issued upon request."     CNY                           

9243544  Autopure, PM Service    "

Service response time (repair): not applicable

Periodic inspection/maintenance: yes

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts : not included

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage" CNY                           

9242713  Add. Unit, Validation Support, 2d   Each additional instrument (EZ1, QIAgility, QIAsymphony, QIAcube, Rotor-Gene Q) to be included in the Guided or Accelerated validation service catalog no(s). Scope of work defined from the primary unit utilized in "Guided Validation Support" or "Accelerated Validation Support".  Refer to the primary part number for additional information. Quantity of additional units is defined by the number of instruments undergoing a Performance Check study to the primary unit utilized in validation.   CNY                           

9242545  Quick Disconnect O-ring set, LC         CNY                           

9242337  Tool, calibration, AS, Qsym          CNY                           

5000-1303      Potentiometer Speed, SKR1          CNY                           

5000-1283      DML 2000 - Power Cord, 220V B Type (IT)              CNY                           

5000-1279      Led Assembly, DML2           CNY                           

5000-1269      Optical Sensor New STY, DML2         CNY                           

5000-1267      Power Transformer, DML2          CNY                            【山羊生长激素释放多肽(GHRP)ELISA试剂盒现货】

5000-1263      PMT Housing W IRIS PCB, DML2             CNY                           

5050-1037      Microplate Heater Interface Cable         CNY                           

5025-1022      Expand 4 Power Supply (English)        CNY                           

6000-4114EN  RCS Tech Reference Guide (English)          CNY