【友情提示】：本产品仅供科研研究使用，不得用于人体临床直接检测。避免给您带来不必要的损失，请仔细阅读购买说明！

本试剂盒仅供研究使用，齐一生物销售：021－6034 8496；181214 53965；173021 04490欢迎选购！

操作步骤

1.准备试剂，样品和标准品

2.加入准备好的样品和标准品，37℃反应30分钟

3.洗板5次，加入酶标试剂，37℃反应30分钟

4.洗板5次，加入显色液A、B，37℃显色15分钟

5.加入终止液

6.15分钟之内读OD值

7.计算
齐一生物供应ELISA试剂盒检测的目的是为实验提供准确可靠的定量分析实验依据。为了保证实验数据的可靠性，在实验过程中必须坚持全面质量控制和全过程质量控制。在收集标本前都必须有一个完整的计划。

【兔热休克蛋白90(HSP-90)ELISA试剂盒厂家】样本保存条件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6个月

3.-50℃以下的冰箱，可以放一年

【兔热休克蛋白90(HSP-90)ELISA试剂盒厂家】样本收集

1.血清样本用干燥管（红色）或者促凝管（黄色）收集

2.血浆用抗凝管，一般有EDTA抗凝（紫色）、枸橼酸钠抗凝（黑色或者蓝色）

3.动物组织匀浆（细胞）：

              1g组织加入9ml PBS（浓度为0.1M，pH为7.4的）测分泌型指标

              1g组织加入1ml组织裂解液加入8ml PBS 测细胞内

粪便样本：1g样本+9ml PBS（浓度为0.01M，pH为7.4的）

没有PBS的话也可以用一下生理盐水，还是用PBS

【兔热休克蛋白90(HSP-90)ELISA试剂盒厂家】样本离心转速及时间

1.试管采集的样本：3000转，离心五分钟取上清

2.离心管或者EP管采集的样本：5000－－8000转3分钟取上清

3..样本保存－－如果样品不立即使用，应将其分成小部分-70 ℃保存，避免反复冷冻。尽可能的不要使用溶血或高血脂血。如果血清中大量颗粒，检测前先离心或过滤。不要在37℃或更高的温度加热解冻。应在室温下解冻并确保样品均匀地充分解冻。

【兔热休克蛋白90(HSP-90)ELISA试剂盒厂家】以上样本采集及处理方式供您参考，如有问题请来电，齐一生物销售：021－6034 8496；181214 53965；173021 04490

291115    mericon RR Soy Kit (96)              CNY                            【兔热休克蛋白90(HSP-90)ELISA试剂盒厂家】

291065    mericon GMO Screen bar (96)            CNY                           

9001933  DML 3000 120-240V Q        CNY                           

9001915  PyroMark Q96 ID, Priority    PyroMark Q96 ID, Priority includes the PyroMark Q96 ID instrument, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with introductory training. Priority package extends warranty period of the system to 2 years. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Service package includes all parts, labor ａnd travel costs with a service response time of 48 hours (2 working days). Performance of the installation ａnd service visits are documented on a GMP/GLP-compliant Field Service documents.       CNY                           

133120    NeXtalStock Tricine (200)           CNY                           

133180    NeXtalStock Citric acid pH 3.0 (200)          CNY                           

9018503  Manual air supply, service tool, QX             CNY                           

51161      QIAamp 96 DNA Blood Kit (4)     For 4 x 96 DNA preps: 4 QIAamp 96 Plates, QIAGEN Protease, Reagents, Buffers, Lysis Blocks, Tape Pads, Collection Vessels   CNY                           

133106    NeXtalStock Sodium ac. H2O pH 4.6 (200)        CNY                           

9015519  Spring, for door            CNY                           

9001897  QIAxtractor, UV/HEPA (w/o PC) Priority     QIAxtractor, UV/HEPA (w/o PC) Priority includes the QIAxtractor instrument, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with introductory training. Priority package extends warranty period of the system to 2 years. One on-site pipetting head service ａnd inspection is included for each year of the warranty; 2 pipetting head services in total. Service package includes all parts, labor ａnd travel costs with an on site service response time of 48 hours (2 working days). Performance of the installation ａnd service visits are documented on a GMP/GLP-compliant Field Service documents.      CNY                           

9001875  EZ1 Advanced XL, Priority    EZ1 Advanced XL, Priority includes the EZ1 Advanced XL instrument, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with introductory training. Priority package extends warranty period of the system to 2 years. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Service package includes all parts, labor ａnd travel costs with a service response time of 48 hours (2 working days). Performance of the installation ａnd service visits are documented on a GMP/GLP-compliant Field Service documents.       CNY                           

959236    MagAttract RNA Tissue Mini M48 Kit (192)        CNY                           

956336    MagAttract RNA Univ. Tissue M48 Kit(192)        CNY                           

9018457  Cartridge, alignment tool, QX              CNY                           

9015467  Motor, P         CNY                           

9015451  Modules, DISU, BR8000 v2.1              CNY                            【兔热休克蛋白90(HSP-90)ELISA试剂盒厂家】

9012427  Thermostate system, complete, 120V, BR30        CNY                           

9018483  Notebook, QX              CNY                           

51297     FlexiGene DNA AGF3000 Kit (640)      "Buffer FG1  = 4 x 825 ml

Buffer FG2 = 1 x 750 ml

Buffer FG3 = 10 x 625 ml

QIAGEN Protease = 5 vials

1 handbook"   CNY                           

51185      QIAamp DNA Blood Midi Kit (100)      For 100 DNA midipreps: 100 QIAamp Midi Spin Columns, QIAGEN Protease, Buffers, Collection Tubes (15 ml) CNY                           

38075     AIL Cartridge (5ml)              CNY                           

9015471  Sensor, photo, PM-U24         CNY                           

956034    EZ1 RNA Universal Tissue Kit (48)      Provides automated purification of total RNA from 1-6 samples of all types of animal or human tissue, including difficult-to-lyse fibrous ａnd fatty tissues, or cultures cells.      CNY                           

204772    Rotor-Gene Multiplex PCR Kit (80)      The Rotor-Gene Multiplex PCR Kit is dedicated for use with the Rotor-Gene Q cycler, providing ultrafast, highly reliable quantification in multiplex, real-time PCR ａnd two-step RT-PCR using sequence-specific probes. Up to 4 genes (e.g., 1 control gene ａnd 3 target genes) can be quantified simultaneously in the same reaction.  CNY                           

9018435  Tubing, external N2 source, 10ft, QX          CNY                           

9018417  Cover, front, blue window, QX            CNY                           

9018403  ZLV, Detect barrel (1), QX           CNY                           

290103    mericon Shigella spp Kit (24)        CNY                           

9015392  Clamp, rubber, adapter plate, HS shaker,             CNY                           

9001793  QIAcube HT/QIAxtractor, system        CNY                           

9012330  Syringe Pump outlet tubing           CNY                           

QKT0574       总胆红素测定试剂盒重氮化法                                      

QKT0575       ａ-L-岩藻糖苷酶AFU检测试剂盒CNPF法                                       

QKT0576       甘油三酯TG检测试剂盒酶比色法                                      

QKT0577       低密度脂蛋白胆固醇LDL检测试剂盒酶直接法                                        

QKT0578       载脂蛋白aLpa检测试剂盒比浊法                                         【兔热休克蛋白90(HSP-90)ELISA试剂盒厂家】

QKT0579       胆固醇CHOL检测试剂盒酶比色法                                      

QKT0580       乳酸脱氢酶LDH检测试剂盒连续监测法                                    

QKT0581       肌酐CREA检测试剂盒PAP肌氨酸氧化酶法                                     

QKT0582       丙型肝炎病毒抗体HCV检测试剂盒胶体金法                                    

QKT0584       吗啡检测试剂MOP检测试剂盒胶体金法                                    

QKT0585       总胆红素测定试剂盒重氮化法                                      

QKT0586       丙型肝炎病毒抗体诊断试剂盒酶联免疫法                                         

QKT0587       甲型肝炎病毒IgM抗体诊断试剂盒酶联免疫法                                         

QKT0588       梅毒甲苯胺红不加热血清试验诊断试剂                                      

QKT0589       人类免疫缺陷病毒抗体诊断试剂盒酶联免疫法                                         

QKT0590       乙肝两对半检测试剂盒酶联免疫法                                      

QKT0591       乙型肝炎病毒核心抗体IgM诊断试剂盒酶联免疫法                                         

QKT0592       吗啡检测试剂MOP检测试剂盒胶体金法                                    

QKT0593       ａ-L-岩藻糖苷酶AFU检测试剂盒CNPF法                                       

QKT0594       甘油三酯TG检测试剂盒酶比色法                                      

QKT0595       低密度脂蛋白胆固醇LDL检测试剂盒酶直接法                                         【兔热休克蛋白90(HSP-90)ELISA试剂盒厂家】

QKT0596       载脂蛋白aLpa检测试剂盒比浊法                                        

QKT0597       抗环瓜氨酸肽抗体测定试剂盒胶乳增强免疫比浊法                                         

QKT0598       脂肪酶测定试剂盒LPS       酶比色法                                  

QKT0599       β-羟丁酸测定试剂盒β-HB       酶法                                  

QKT0600       二氧化碳测定试剂盒CO2   酶法                                  

QKT0601       锌测定试剂盒Zn   比色法铜测定试剂盒                               

QKT0602       缺血修饰白蛋白测定试剂盒IMA       白蛋白-钴结合试验法                             

QKT0603       C反应蛋白测定试剂盒CRP       胶乳增强免疫比浊法                               

QKT0604       铁测定试剂盒Fe   比色法                               

QKT0570       同型半胱氨酸检测试剂盒HCY酶法                                    

QKT0571       胱抑素CCys-C检测试剂盒免疫比浊法                                       

QKT0572       糖化血红蛋白HbA1c检测试剂盒酶法                                        

QKT0573       二氧化碳CO2检测试剂盒酶法