1. 酶标仪(450nm 波长滤光片)

2. 高精度移液器，EP 管及一次性吸头

3. 37℃恒温箱，双蒸水或去离子水

4. 吸水纸

【兔儿茶酚胺(CA)ELISA试剂盒Rabbitcatecholamine,CAELISA试剂盒厂家】齐一生物【友情提示】：本产品仅供科研研究使用，不得用于人体临床直接检测。避免给您带来不必要的损失，请仔细阅读购买说明！

ELISA应用：

ELISA检测试剂盒、ELISAKit、酶联免疫试剂盒、酶联免疫吸附测定试剂盒、酶联免疫分析试剂盒、酶免试剂盒等,比较常见的叫法是ELISA检测试剂盒、酶联免疫吸附测定试剂盒等ELISA试剂盒自从60-70年代问世以来,得到全世界科研工作者的认可及推崇,在欧美及中国获得很大的推广,尤其是国内生化领域的长足发展.Elisa生物试验是一种敏感性高,特异性强,重复性好的实验诊断方法.由于其试剂稳定、易保存,操作简便,结果判断较客观等因素,已广泛应用在免疫学检验的各领域中.ELISA检测试剂盒适用于体外定性检测人血清或血浆中的抗人类戊型肝炎（HEV）病毒IgM抗体ELISA检测

【兔儿茶酚胺(CA)ELISA试剂盒Rabbitcatecholamine,CAELISA试剂盒厂家】齐一生物科技欢迎您订购咨询，齐一生物销售：021－6034 8496；181214 53965；173021 04490

齐一生物供应的ELISA试剂盒安全性提示：

1.工作人员实验中避免直接接触终止液和底物A、B。一旦接触到这些液体，请尽快用水冲洗。

2.实验中不要吃喝、抽烟或使用化妆品。

3.不要用嘴吸取试剂盒里的任何成份。

样本保存条件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6个月

3.-50℃以下的冰箱，可以放一年

【兔儿茶酚胺(CA)ELISA试剂盒Rabbitcatecholamine,CAELISA试剂盒厂家】齐一生物供应的ELISA试剂盒ELISA实验原理

试剂盒是固相夹心法酶联免疫吸附实验（ELISA）.已知浓度的标准品、未知浓度的样品加入微孔酶标板内进行检测.先将生物素标记的抗体同时温育.洗涤后,加入亲和素标记过的HRP.再经过温育和洗涤,去除未结合的酶结合物,然后加入底物A、B,和酶结合物同时作用.产生颜色.颜色的深浅和样品中的浓度呈比例关系.

免疫测定技术的基础在于抗原抗体之间的特异结合反应,所以任何优质的诊断试剂离不开优质的原料,如抗原抗体,酶等.以前用于免疫测定的抗原通常为各种纯化抗原,而抗体则为纯化抗原免疫动物后获得的多克隆抗体和使用杂交瘤技术得到的单克隆抗体,而抗原或抗体的标记物如酶标结合物则通过各种化学合成方法制备.近年来,随着分子生物学的发展,使用基因工程方法制备各种特殊的抗原或抗体及其酶结合物等免疫测定试剂几成为现实的新一代试剂,各种新型使用的测定方法也不断出现.

HBsAg试剂特点（检测模式：临床抗体夹心法）：包被抗体为山羊多抗.多抗具有高亲和性和对抗原各种表位的反应性.酶表抗体为与HBsAg有不同结合位点的鼠复合单位,可测得HBsAg变异样品,提高检测的特异性（99.98%）提高检测的灵敏度,应用ParlEhrich学说（PEI）HBsAg标准品,对ad标准品的灵敏度为0.05ng/ml对ay标准品灵敏度为0.025ng/ml

ELISA检测试剂盒应用定性夹心免疫检测技术,用合成的HEV多肽抗原包被微孔板板条,这些多肽是中国型HEV毒株核心氨基酸序列中抗原性很强的肽段,分别来自于该毒株的开放阅读框2和开放阅读框3.将样品或标准品加入孔中并孵育,如果其中存在HEVIgM抗体,这些抗体就会与HEV的多肽抗原结合,并固定在上面,洗板除去其它非特异性抗体和样品中的其它成份.然后加入羊抗人IgM-HRP（辣根过氧化物酶）酶结合物,经第二次孵育后,酶结合物就会与次孵育结合上的HEVIgM抗体相结合,洗板除去未结合的酶结合物,加入TMB底物溶液,在第三次孵育时会发生酶-底物反应,只有那些含有HEVIgM抗体和酶结合物所形成的复合物的孔才会发生颜色变化,加入硫酸溶液终止酶和底物间的反应,并在波长:450nm处测量O.D.值,按照本HEVIgM抗体elisa试剂盒的测试标准,O.D.值大于或等于Cut-Off值的样品被认为是初试阳性. 【兔儿茶酚胺(CA)ELISA试剂盒Rabbitcatecholamine,CAELISA试剂盒厂家】

以上资料仅供参考了解，不供于论文写作

齐一生物专业为您提供高品质，高灵敏度.高质量的酶联免疫试剂盒，齐一生物长期经营ELISA试剂盒（免费代测）及抗体、分子学生物试剂盒、金标法检测试剂盒、比色法检测试剂盒、细胞菌株、培养基、细胞因子、生化试剂、耗材等生物试剂产品。齐一生物销售：021－6034 8496；181214 53965；173021 04490

38071       AIL Cartridge (1ml)           CNY                              【兔儿茶酚胺(CA)ELISA试剂盒Rabbitcatecholamine,CAELISA试剂盒厂家】

38015       #WGA Cartridge (5ml)               CNY                             

204754     QuantiFast Multiplex PCR +R Kit (400)              CNY                             

204643     QuantiTect Multiplex RT-PCR Kit (200)             CNY                             

204574     Rotor-Gene Probe RT-PCR Kit (400)        The Rotor-Gene Probe RT-PCR Kit is dedicated for use with the Rotor-Gene Q cycler, providing ultrafast, highly sensitive quantification in real-time one-step RT-PCR using sequence-specific probes.         CNY                            

204363     QuantiTect Probe PCR + UNG Kit (200)   The supplied, ready-to-use ｍaster mix contains HotStarTaq DNA Polymerase, ROX passive reference dye, ａnd a dNTP mix in an optimized buffer. The dNTP mix includes dUTP, allowing optional treatment with UNG. The UNG enzyme is provided separately. For convenience, the ｍaster mix can be stored at 2–8°C.     CNY                             

9015350  Board, terminal                 CNY                             

9015349  Board, motor controller           CNY                             

9018402  Kit, purge filter, QX           CNY                             

9018389  QIAcube Tip Adapter Ring Replacement Kit            CNY                            

9012283  Power Supply, 6 amp for P 90, (7135)                CNY                             

9012180  Syringe pump inlet tubing                  CNY                             

9012034  Base plate, gripper motor, handler, BR80                 CNY                             

950254     Wash Reagent (VXW) (650 ml)                  CNY                             

950240     Binding Additive (VX) (4.0 g)             CNY                             

38001       ConA Cartridge (1ml)                 CNY                             

678291     ipsogen MLL-AF4 e11e5 Standards           CNY                              【兔儿茶酚胺(CA)ELISA试剂盒Rabbitcatecholamine,CAELISA试剂盒厂家】

678091     ipsogen MLL-AF1p e10e2 Standards                  CNY                             

34705       6xHis Protein Ladder        6xHis-tagged marker proteins (lyophilized, for 50-100 lanes on western blots)      CNY                            

33223       Factor Xa Protease (400 Units)         400 units Factor Xa Protease (2 units/ul)          CNY                             

30622       Ni-NTA Superflow Columns (12 x 1.5 ml) For 12 6xHis-tagged protein preps: 12 polypropylene columns containing 1.5 ml Ni-NTA Superflow     CNY                             

9021359  Rubber (3), spring foot, TLyser LT              CNY                             

9013184  Tubing, retractable tubing, DISU, BR8000                CNY                             

5000-1512        L19 FIRMWARE, DML2             CNY                             

6000-2240E      Rotary Shaker I, 240V               CNY                             

9240988  Solenoide, handler, Qsym                  CNY                             

677013     ipsogen NPM1 MutaScreen Kit (24)                  CNY                             

676991     ipsogen WT1 ProfileQuant Standards                CNY                             

9011327  Motor,  X, with gearbox + encoder                 CNY                             

9011237  Kabel Level Sensor PK80           CNY                             

9011220  Cover, bridge, BR8000              CNY                             

9010982  Shaker unit, 1-plate, color code 9170               CNY                             

EK0437     hLeptin-ELISA  人瘦素酶联免疫试剂盒         Human Leptin ELISA Kit   96 tests                       【兔儿茶酚胺(CA)ELISA试剂盒Rabbitcatecholamine,CAELISA试剂盒厂家】

EK0439     hLeptinR-ELISA         人瘦素受体酶联免疫试剂盒         Human Leptin receptor ELISA Kit    96 tests                      

EK0444     hM-CSF-ELISA  人巨噬细胞集落刺激因子酶联免疫试剂盒         Human M-CSF ELISA Kit   96 tests                      

EK0450     hCCL4-ELISA     人CCL4/MIP-1β 酶联免疫试剂盒        Human CCL4/MIP-1 beta ELISA Kit  96 tests                      

EK0458     hMMP-1-ELISA         人 MMP-1酶联免疫试剂盒 Human MMP-1 ELISA Kit          96 tests                      

EK0459     hMMP-2-ELISA         人 MMP-2酶联免疫试剂盒 Human MMP-2 ELISA Kit          96 tests                      

EK0461     hMMP3-ELISA 人MMP-3酶联免疫试剂盒   Human MMP-3 ELISA Kit          96 tests                      

EK0465     hMMP9-ELISA 人MMP-9酶联免疫试剂盒   Human MMP-9 ELISA Kit          96 tests                      

EK0468     hMMP13-ELISA        人MMP-13 酶联免疫试剂盒        Human MMP-13 ELISA Kit       96 tests                      

EK0469     hNGF-ELISA      人神经生长因子/神经生长因子β 酶联免疫试剂盒 Human NGF/NGFβ ELISA Kit         96 tests                     

EK0472     hNT3-ELISA      人神经营养因子-3 酶联免疫试剂盒   Human Neurotrophin-3 ELISA Kit    96 tests                      

EK0482     hOPN-ELISA      人OPN酶联免疫试剂盒        Human OPN ELISA Kit      96 tests                      

EK0484     hPDGF-AB-ELISA      人PDGF-AB酶联免疫试剂盒         Human PDGF-AB ELISA Kit       96 tests                      

EK0494     hRantes-ELISA 人趋化因子酶联免疫试剂盒         Human Rantes ELISA Kit 96 tests                      

EK0501     hSELE-ELISA     人E选择素Elisa试剂盒         Human E-Selectin ELISA Kit     96 tests                      

EK0503     hSELL-ELISA      人L选择素Elisa试剂盒         Human L-Selectin ELISA Kit      96 tests                      

EK0505     hSELP-ELISA     人P选择素Elisa试剂盒         Human P-Selectin ELISA Kit     96 tests                       【兔儿茶酚胺(CA)ELISA试剂盒Rabbitcatecholamine,CAELISA试剂盒厂家】

EK0511     hTGF-α-ELISA 人TGFαElisa试剂盒      Human TGFα ELISA Kit 96 tests                      

EK0513     hTGFβ1-ELISA         人TGF-β1Elisa试剂盒  Human TGF-β1 ELISA Kit        96 tests                      

EK0525     hTNF-ELISA       人TNFElisa试剂盒 Human TNF ELISA Kit       96 tests                      

EK0528     hTNFR1-ELISA  人TNFsRIElisa试剂盒     Human TNFsRI ELISA Kit 96 tests                      

EK0530     hTNFsRII-ELISA         人可溶性肿瘤坏死因子受体IIElisa试剂盒 Human Soluble TNFRII ELISA Kit      96 tests                      

EK0532     hTRAIL-ELISA   人TRAILElisa试剂盒       Human TRAIL ELISA Kit   96 tests                      

EK0535     huPA-ELISA       人uPA/PLAUElisa试剂盒        Human uPA/PLAU ELISA Kit     96 tests                      

EK0536     huPAR-ELISA    人uPARElisa试剂盒        Human uPAR ELISA Kit     96 tests                      

EK0537     hVCAM-ELISA  人VCAM-1Elisa试剂盒  Human VCAM-1 ELISA Kit        96 tests                      

EK0539     hVEGF-ELISA    人血管内皮生长因子ELISA试剂盒      Human VEGF ELISA Kit    96 tests                      

EK0544     hVEGFR-ELISA 人VEGFR2/KDRELISA试剂盒 Human VEGFR2/KDR ELISA Kit         96 tests                      

EK0561     hECad-ELISA     人钙粘附蛋白ELISA试剂盒  Human E-Cadherin ELISA Kit   96 tests                      

EK0563     hCT1-ELISA       人心营养素-1ELISA试剂盒    Human Cardiotrophin-1 ELISA Kit    96 tests                      

        讲述完了以上ELISA实验的操作要点，可以发现其实很多步骤都并不复杂，重要的是操作的时候一定要注重细节，熟能生巧，才可以掌握ELISA实验的精髓所在。

949012     Autopure Proteinase K Solution (3500 µl)                  CNY                              【兔子主要组织相容性复合体Ⅲ类(MHCⅢ/RLAⅢ)ELISA试剂盒厂家】

990395     Starter Pack, QIAcube               CNY                             

336312     SureSilencing shRNA Plasmid, Hygromycin               CNY                             

336311     SureSilencing shRNA Plasmid, GFP            CNY                             

990390     Reagent Bottle Rack                 CNY                             

941517     BioSprint 15 DNA Plant Kit (360)               CNY                             

330629     RT² SYBR Green ROX FAST Mastermix (25ml)          CNY                             

1070210  Snap-on connector (1)               CNY                             

979203     PyroMark Control Oligo   For installation check of PyroMark instrument.       CNY                             

9012986  Thermostate system,complete,(220V), 9604           CNY                             

9012937  RCS - Reagent Trough                CNY                             

9012805  Tube Holder, STS, conical, 2ml (6)             CNY                             

330610     RT² SYBR Green Fluor FAST Mastermix (2)                CNY                             

330519     RT² SYBR Green Fluor qPCR Mastermix 25ml           CNY                             

929704     QX Colored 0.2 ml 12-Tube Strip (80)                 CNY                             

330509     RT² SYBR Green qPCR Mastermix (25 ml)                 CNY                             

336391     SureSilencing shRNA Control             CNY                              【兔子主要组织相容性复合体Ⅲ类(MHCⅢ/RLAⅢ)ELISA试剂盒厂家】

336161     Multi-Analyte  ELISArray Kit           CNY                             

331242     miScript PreAMP Pathway HC Primer mix                 CNY                             

979206     PyroMark Q24 Vacuum Prep Troughs (12)       small plastic containers for reagents during vacuum work      CNY                            

979204     PyroMark Q24 Validation Oligo        For verification of proper installation of PyroMark Q24  CNY                             

133255     NeXtalStock HEPES sod. salt pH 7.2 (200)                 CNY                             

133254     NeXtalStock HEPES sod. salt pH 7.0 (200)                 CNY                             

330522     RT² SYBR Green ROX qPCR Mastermix (12)              CNY                             

330411     RT² HT First Strand Kit (96)               CNY                             

67163       MagAttract 96 DNA Plant Core Kit (24)  MagAttract Suspension A ａnd buffers for 24 x 96 preps CNY                             

210212     QIAGEN OneStep RT-PCR Kit (100)  For 100 reactions: QIAGEN OneStep RT-PCR Enzyme Mix, 5x QIAGEN OneStep RT-PCR Buffer,* dNTP Mix,** 5x Q-Solution, RNase-free water CNY                             

9012771  Liquid Handling Kit Plato 7382/7182                  CNY                             

9010359  Cable set, card-X to bar-code motors, 70                  CNY                             

9010346  Cable, communication Y/Z CPU                 CNY                             

310007     BioMag Goat Anti-Mouse IgG (500ml)    BioMag goat anti-mouse IgG secondary antibody suspension (1 mg/ml)        CNY                             

301802     HiPerFect HTS Reagent (0.1 ml)                CNY                             

QKT0942  羊源性成分核酸检测试剂盒PCR-荧光探针法                                                  

QKT0943  登革热病毒DENV IV型病毒核酸检测试剂盒荧光法                                              

QKT0944  呼吸道21种病原体荧光检测试剂盒                                                

QKT0945  强化型呼吸系统病原体荧光检测试剂盒                                                  

QKT0947  细菌性肺炎荧光检测试剂盒                                                       

QKT0948  呼吸系统33种病原体荧光检测试剂盒                                                     

QKT0950  博德氏菌荧光检测试剂盒                                                  

QKT0951  流感荧光检测试剂盒                                                  

QKT0952  ACE荧光检测试剂盒                                                   

QKT0953  巨细胞病毒荧光检测试剂盒                                                       

QKT0954  病毒性肠胃炎荧光检测试剂盒                                                  

QKT0955  细菌性肠胃炎荧光检测试剂盒                                                  

QKT0956  肠内寄生虫荧光检测试剂盒                                                        【兔子主要组织相容性复合体Ⅲ类(MHCⅢ/RLAⅢ)ELISA试剂盒厂家】

QKT0957  消化系统荧光检测试剂盒                                                  

QKT0958  艰难梭状杆菌荧光检测试剂盒                                                  

QKT0959  丙肝病毒荧光检测试剂盒                                                  

QKT0960  丙肝病毒荧荧光检测试剂盒                                                       

QKT0961  疱疹病毒荧光检测试剂盒                                                  

QKT0962  病毒性脑膜炎荧光检测试剂盒                                                  

QKT0963  细菌性脑膜炎荧光检测试剂盒                                                  

QKT0964  腮腺炎荧光检测试剂盒                                                       

QKT0965  尿道炎荧光检测试剂盒                                                       

QKT0966  淋病荧光检测试剂盒                                                  

QKT0967  乙型肝炎病毒核心IgM抗体检测试剂盒                                                  

EK0301     hActA-ELISA     人活化素A酶联免疫试剂盒         Human Activin A ELISA Kit       96 tests                      

EK0305     hANG-ELISA     人双调蛋白酶联免疫试剂盒         Human ANG ELISA Kit      96 tests                      

EK0307     hBDNF-ELISA   人脑源性神经生长因子酶联免疫试剂盒     Human BDNF ELISA Kit    96 tests                      

EK0310     hBMP-5-ELISA 人BMP-5酶联免疫试剂盒    Human BMP-5 ELISA Kit  96 tests                      

EK0311     hBMP-2-ELISA 人BMP-2酶联免疫试剂盒    Human BMP-2 ELISA Kit  96 tests                       【兔子主要组织相容性复合体Ⅲ类(MHCⅢ/RLAⅢ)ELISA试剂盒厂家】

EK0314     hBMP-4-ELISA 人BMP-4酶联免疫试剂盒    Human BMP-4 ELISA Kit  96 tests