1. 酶标仪(450nm 波长滤光片)

2. 高精度移液器，EP 管及一次性吸头

3. 37℃恒温箱，双蒸水或去离子水

4. 吸水纸

【兔子胶原酶I(CollagenaseI)ELISA试剂盒厂家】齐一生物【友情提示】：本产品仅供科研研究使用，不得用于人体临床直接检测。避免给您带来不必要的损失，请仔细阅读购买说明！

ELISA应用：

ELISA检测试剂盒、ELISAKit、酶联免疫试剂盒、酶联免疫吸附测定试剂盒、酶联免疫分析试剂盒、酶免试剂盒等,比较常见的叫法是ELISA检测试剂盒、酶联免疫吸附测定试剂盒等ELISA试剂盒自从60-70年代问世以来,得到全世界科研工作者的认可及推崇,在欧美及中国获得很大的推广,尤其是国内生化领域的长足发展.Elisa生物试验是一种敏感性高,特异性强,重复性好的实验诊断方法.由于其试剂稳定、易保存,操作简便,结果判断较客观等因素,已广泛应用在免疫学检验的各领域中.ELISA检测试剂盒适用于体外定性检测人血清或血浆中的抗人类戊型肝炎（HEV）病毒IgM抗体ELISA检测

【兔子胶原酶I(CollagenaseI)ELISA试剂盒厂家】齐一生物科技欢迎您订购咨询，齐一生物销售：021－6034 8496；181214 53965；173021 04490

齐一生物供应的ELISA试剂盒安全性提示：

1.工作人员实验中避免直接接触终止液和底物A、B。一旦接触到这些液体，请尽快用水冲洗。

2.实验中不要吃喝、抽烟或使用化妆品。

3.不要用嘴吸取试剂盒里的任何成份。

样本保存条件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6个月

3.-50℃以下的冰箱，可以放一年

【兔子胶原酶I(CollagenaseI)ELISA试剂盒厂家】齐一生物供应的ELISA试剂盒ELISA实验原理

试剂盒是固相夹心法酶联免疫吸附实验（ELISA）.已知浓度的标准品、未知浓度的样品加入微孔酶标板内进行检测.先将生物素标记的抗体同时温育.洗涤后,加入亲和素标记过的HRP.再经过温育和洗涤,去除未结合的酶结合物,然后加入底物A、B,和酶结合物同时作用.产生颜色.颜色的深浅和样品中的浓度呈比例关系.

免疫测定技术的基础在于抗原抗体之间的特异结合反应,所以任何优质的诊断试剂离不开优质的原料,如抗原抗体,酶等.以前用于免疫测定的抗原通常为各种纯化抗原,而抗体则为纯化抗原免疫动物后获得的多克隆抗体和使用杂交瘤技术得到的单克隆抗体,而抗原或抗体的标记物如酶标结合物则通过各种化学合成方法制备.近年来,随着分子生物学的发展,使用基因工程方法制备各种特殊的抗原或抗体及其酶结合物等免疫测定试剂几成为现实的新一代试剂,各种新型使用的测定方法也不断出现.

HBsAg试剂特点（检测模式：临床抗体夹心法）：包被抗体为山羊多抗.多抗具有高亲和性和对抗原各种表位的反应性.酶表抗体为与HBsAg有不同结合位点的鼠复合单位,可测得HBsAg变异样品,提高检测的特异性（99.98%）提高检测的灵敏度,应用ParlEhrich学说（PEI）HBsAg标准品,对ad标准品的灵敏度为0.05ng/ml对ay标准品灵敏度为0.025ng/ml

ELISA检测试剂盒应用定性夹心免疫检测技术,用合成的HEV多肽抗原包被微孔板板条,这些多肽是中国型HEV毒株核心氨基酸序列中抗原性很强的肽段,分别来自于该毒株的开放阅读框2和开放阅读框3.将样品或标准品加入孔中并孵育,如果其中存在HEVIgM抗体,这些抗体就会与HEV的多肽抗原结合,并固定在上面,洗板除去其它非特异性抗体和样品中的其它成份.然后加入羊抗人IgM-HRP（辣根过氧化物酶）酶结合物,经第二次孵育后,酶结合物就会与次孵育结合上的HEVIgM抗体相结合,洗板除去未结合的酶结合物,加入TMB底物溶液,在第三次孵育时会发生酶-底物反应,只有那些含有HEVIgM抗体和酶结合物所形成的复合物的孔才会发生颜色变化,加入硫酸溶液终止酶和底物间的反应,并在波长:450nm处测量O.D.值,按照本HEVIgM抗体elisa试剂盒的测试标准,O.D.值大于或等于Cut-Off值的样品被认为是初试阳性. 【兔子胶原酶I(CollagenaseI)ELISA试剂盒厂家】

以上资料仅供参考了解，不供于论文写作

齐一生物专业为您提供高品质，高灵敏度.高质量的酶联免疫试剂盒，齐一生物长期经营ELISA试剂盒（免费代测）及抗体、分子学生物试剂盒、金标法检测试剂盒、比色法检测试剂盒、细胞菌株、培养基、细胞因子、生化试剂、耗材等生物试剂产品。齐一生物销售：021－6034 8496；181214 53965；173021 04490

9240531  Cable, Motor, Axis #7, Pump U/D, AP98           CNY                              【兔子胶原酶I(CollagenaseI)ELISA试剂盒厂家】

9240564  Fan, Condenser, 230V,Eppendorf, AP196/98              CNY                             

9240553  Cable, PC-104 to Eppendorf, AP196/98              CNY                             

9240548  Motor Cap Repair, Eppendorf, AP196/98          CNY                             

9240515  I/O PCB, Main Board, AP196/98                 CNY                             

9240503  PM Kit, AP196          CNY                             

9239958  BR M48, PM Service         "Service response time (repair): not applicable

Periodic inspection/maintenance: yes

 Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts : not included

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage"  CNY                             

9239853  Foot, complete, v2.0 (4)           CNY                             

9239572  BR Universal, Full Agreement The Full Agreement BR Universal provides full service coverage for the BioRobot Universal System. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 48 hours (2 working days). CNY                             

9239563  Assy,Kit,Retrofit,HoriCompressorMnt              CNY                             

9020033  LED UV (325), RGQ           CNY                             

9020023  L 26_7 Firmware, DML2           CNY                             

9019975  PCB, UV light assembly, Cxx              CNY                             

9238858  Basic Cover Agreement Plato7, 1yr          The Basic Cover Agreement Plato7 provides full service coverage for the Plato 7 platform. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.         CNY                             

9241233  User Manual, QIAxcel FA 01/08 (WW)                CNY                             

9241227  ZLV, Handscanner, Qsym          CNY                             

9241180  Motor, Z, QX             CNY                              【兔子胶原酶I(CollagenaseI)ELISA试剂盒厂家】

9019473  Loading guide, 52 well sharkstooth, GSC                   CNY                             

9019462  Switch, micro, HS              CNY                             

9019457  Fans (2), 5V, 30mm, HS             CNY                             

9237172  Cable USB                  CNY                             

9233169  Board, YZ-CPU, BR8000            CNY                             

9237163  Bezel LiquiChip                   CNY                             

9237162  Shipping Plug XYP              CNY                             

9237135  #Twister I, Full Agreement, no PM The Full Agreement BR Twister I provides full service coverage for the BioRobot Twister I. Costs for service visits are fully covered including travel, labor, ａnd parts. With a Full Agreement, there is a priority response time of 48 hours (2 working days).       CNY                             

9237109  Protocol Dev./Opt., on-site (5-days)         The QIAGEN BioRobot Systems Protocol Development/Optimization On-Site Visit includes all travel ａnd labor charges for a site visit up to 5 days by a QIAGEN Instrumentation Service Specialist to write and/or opimize protocols for the BioRobot System. Parts, consumable chemistries, ａnd accessories not included. Custom protocols are the sole responsibility of the customer ａnd neither warrantied nor guaranteed.          CNY                             

9237102  Upgrade, BR plus 2 ext. devices       Upgrade BioRobot with two approved integration devices    CNY                             

9232974  Card, x-main, peristaltic XPUPG                CNY                             

9232972  YZ 96 CPU Board/Driver Unit            CNY                             

9232947  Springcontact, RoboVac, BR8000             CNY                             

9236408  Storage Platform TW I, deep well             CNY                             

9232918  Board, PCON, auto vac, BR8000               CNY                             

QY-L0711 秦皮素574-84-5      Fraxetin   574-84-5  20mg                          

QY-L0712 秦皮乙素305-01-1 Esculetin  305-01-1  20mg                          

QY-L0713 秦皮苷524-30-1      fraxin        524-30-1  20mg                          

QY-L0714 千金子二萜醇34420-19-4      Lathyrol   34420-19-4       20mg                          

QY-L0715 千金藤素481-49-2 Cepharanthine          481-49-2  20mg                           【兔子胶原酶I(CollagenaseI)ELISA试剂盒厂家】

QY-L0716 千金子甾醇（95%）28649-59-4   Euphobiasteroid       28649-59-4       20mg                          

QY-L0717 千层纸素A480-11-5        Oroxylin A         480-11-5  20mg                          

QY-L0718 青阳参苷元 84745-94-8         Qingyangshengenin         84745-94-8       20mg                          

QY-L0719 10-羟基癸烯酸14113-05-4    10-Hydroxy-2-decenoic acid   14113-05-4       20mg                          

QY-L0720 乌药醚内酯13476-25-0 Linderane         13476-25-0       20mg                          

QY-L0721 去甲异波尔定5890-18-6        Laurolitsine      5890-18-6         20mg                          

QY-L0722 奇壬醇52659-56-0 kirenol      52659-56-0       20mg                          

QY-L0723 5-羟基色氨酸56-69-9    5-Hydroxytryptophan       56-69-9    20mg                          

QY-L0724 芦丁153-18-4 Rutin       153-18-4  20mg                          

QY-L0725 曲克芦丁7085-55-4        Troxerutin         7085-55-4         20mg                          

QY-L0726 七叶皂苷钠20977-05-3 Sodium Aescinate    20977-05-3       20mg                          

QY-L0727 七叶皂苷A123748-68-5 Aescin IA          123748-68-5    20mg                          

QY-L0728 盐酸去氢骆驼蓬碱343-27-1        Harmine Hydrochloride   343-27-1  20mg                          

QY-L0729 去氢骆驼蓬碱442-51-3 Harmine  442-51-3  20mg                          

QY-L0730 5-羟甲基糠醛67-47-0    5-Hydroxymethylfurfural 67-47-0    20mg                          

QY-L0731 芹菜素-7-O-β-D-吡喃葡萄糖苷578-74-5     Apigenin 7-glucoside        578-74-5  20mg                           【兔子胶原酶I(CollagenaseI)ELISA试剂盒厂家】

QY-L0732 芫花素437-64-9      Genkwanin       437-64-9  20mg                          

QY-L0733 羟基芫花素20243-59-8 Hydroxygenkwanin  20243-59-8       20mg                          

QY-L0734 乔松素480-39-7    Ponocembrin   480-39-7          20mg                          

QY-L0735 桤木酮33457-62-4 Alnusone  33457-62-4       20mg                          

QY-L0736 13-去羟基印乌碱 13-Dehydroxyindaconintine         20mg                          

QY-L0737 印乌头4491-19-4   Indaconitone   4491-19-4         20mg                          

QY-L0738 去氢荷包牡丹碱     Dehydrodicentrine            20mg                          

QY-L0739 5-羟基-7,8-二甲氧基黄酮3570-62-5     5-hydroxy-7,8-dimethoxyflavone     3570-62-5         20mg                          

QY-L0740 5-羟基-6,7-二甲氧基黄酮740-33-0       "5-Hydroxy-6,7-dimethoxylflavone

"       "740-33-0

"       20mg