价格详细资料：试剂盒价格电议，齐一生物销售：021－6034 8496；181214 53965；173021 04490,齐一生物科技（上海）有限公司提供ELISA试剂盒受到了广大研究所.中科院科研单位一致肯定和认同。齐一生物大品牌保证，倾力为国内外科研院校实验室提供*优质产品。

【兔子巨噬细胞炎性蛋白1α(MIP-1α/CCL3)ELISA试剂盒现货】注意事项

1. 当混合蛋白溶液时应尽量轻缓，避免起泡。

2. 洗涤过程非常重要，不充分的洗涤易造成假阳性。

3. 一次加样时间控制在5分钟内，如标本数量多，推荐使用排枪加样。

4. 请每次测定的同时做标准曲线，做复孔。

5. 如标本中待测物质含量过高，请先稀释后再测定，计算时请*后乘以稀释倍数。

6. 在配制标准品、检测溶液工作液时，请以相应的稀释液配制，不能混淆。

7. 底物请避光保存。

8. 不要用其它生产厂家的试剂替换试剂盒中的试剂。

FOR RESEARCH USE ONLY

Drug Names

Generic Name：

This kit can be used for determination of serum, plasma ａnd liquid samples Organization Content.

The experimental principle:

The product levels were measured in samples of the kit by double antibody sandwichmethod. The product with the purified antibody coated microtiter plate, made of solid phase antibody, to package is the product antigen monoclonal antibodies are then added to the micropores, the product and then with HRP labeled antibody binding, the formation of antibody - antigen - antibody complex enzyme label, after thorough washing with TMB chromogenic substrate. TMB in the HRP enzyme catalytic conversion into the blue, and in the action of acid into the final yellow. This product is positively related to the depth of color and in the samples. Instrument measured absorbance in the 450nm wavelength with ELISA (OD), the product concentration in the samples was calculated by standard curve.

Materials provided with the kit

Materials provided with the kit 96 determinations Storage

User manual   1  

Closure plate membrane   2  

Sealed bags   1  

Microelisa stripplate    1   2-8℃

Standard：360ng/L 0.5ml×1 bottle   2-8℃

Standard diluent  1.5ml×1 bottle   2-8℃

HRP-Conjugate reagent    6ml×1 bottle 2-8℃

Sample diluent    6ml×1 bottle 2-8℃

Chromogen Solution A 6ml×1 bottle 2-8℃

Chromogen Solution B 6ml×1 bottle 2-8℃

Stop Solution 6ml×1 bottle 2-8℃

wash  solution    （20ml×30 fold）

×1bottle  2-8℃

Specimen requirements

1.    serum- coagulation at room temperature 10-20 mins，centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. 【兔子巨噬细胞炎性蛋白1α(MIP-1α/CCL3)ELISA试剂盒现货】

2.    plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

3.    Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax ａnd cerebrospinal fluid Reference to it.

4.    cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS（PH7.2-7.4）, Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells ａnd release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

5.    Tissue samples- After cutting samples, check the weight,add PBS（PH7.2-7.4）, Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS（PH7.4）, Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant.

6.    extract as soon as possible after Specimen collection,and according to the relevant literature, ａnd should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.

7.    Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.

Assay procedure

1.Dilute ａnd add sample to Standard: set 10 Standard wells on the ELISA plates coated, add Standard 100μl to the first ａnd the second well, then add Standard dilution 50μl to the first ａnd the second well, mix; take out 100μl form the first ａnd the second well then add it to the third ａnd the forth well separately. then add Standard dilution 50μl to the third ａnd the forth well ,mix ; then take out 50μl from the third ａnd the forth well discard, add 50μl to the fifth ａnd the sixth well ,then add Standard dilution 50μl to the fifth ａnd the sixth well, mix ; take out 50μl from the fifth ａnd the sixth well ａnd add to the seventh ａnd the eighth well, then add Standard dilution 50μl to the seventh ａnd the eighth well ,mix ; take out 50μl from the seventh ａnd the eighth well ａnd add to the ninth ａnd the tenth well, add Standard dilution 50μl to the ninth ａnd the tenth well, mix , take out 50μl from the ninth ａnd the tenth well discard(add Sample 50μl to each well after Diluting ,(density: 240ng/L,160ng/L ,80ng/L,40ng/L, 20ng/L)

2.add sample：Set blank wells separately (blank comparison wells .don’t add sample ａnd HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, ａnd Gently mix. 【兔子巨噬细胞炎性蛋白1α(MIP-1α/CCL3)ELISA试剂盒现货】

3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.

4.Configurate liquid: 30-fold (or 20-fold)wash solution diluted 30-fold (or 20-fold) with distilled water ａnd reserve.

5.washing：Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.

6.add enzyme：Add HRP-Conjugate reagent 50μl to each well, except  blank well.

7.incubate：Operation with 3.

8.washing：Operation with 5.

9.color：Add Chromogen Solution A 50ul ａnd Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃

10.Stop the reaction：Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).

11.assay：take blank well as zero , Read absorbance at 450nm after Adding Stop Solution ａnd within 15min.

Important notes

1.    The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.

2.    washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.

3.    add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 mins, if the number of sample is much , recommend to use Volley . 【兔子巨噬细胞炎性蛋白1α(MIP-1α/CCL3)ELISA试剂盒现货】

4.    if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente ａnd multiplied by the dilution factor.（×n×5）.

5.    Closure plate membrane only limits the disposable use, to avoid cross-contamination.

6.    The substrate evade the light preservation.

7.    Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.

8.    All samples, washing buffer ａnd each kind of reject should according to infective material process.

9.    Do not mix reagents with those from other lots.

Calculate：

Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density ａnd the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.

Storage ａnd validity

1．Storage：  2-8℃.

2．validity： six months.

9239852  Foot bumpers,  v2.0 (4)          CNY                              【兔子巨噬细胞炎性蛋白1α(MIP-1α/CCL3)ELISA试剂盒现货】

9019944  Cable, P-axis, wires, 8 ch. , C18                 CNY                             

9238880  Bearings up. & low, BS96                   CNY                             

9238836  BioSprint96, Basic Agreement, no PM    "Repair service delivery: onsite

Service response time (repair): 5 business days

Periodic inspection/maintenance: not included

Inspection/maintenance delivery: not applicable

Cost coverage for Repair Parts : yes, full coverage

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: not applicable"        CNY                             

9241211  Full Agreement Depot Repair, DML2000, 1y  "The Full Agreement Depot Repair, DML2000 provides full service coverage for the DML 2000 luminometer. The service is conducted as an instrument exchange service.

All shipping, labor ａnd repair part costs related to an instrument exchange are included for 1 year. Instrument exchange time is 2 - 3 working days."          CNY                             

9241191  Magnet, tool, QX              CNY                             

9238825  Spare bucket for plate rotor             CNY                             

9238815  Rect. carrier for 12 tubes 10 ml w/ cap            CNY                             

9019472  TAPE SPACER CLEAR GS             CNY                             

9019464  UV tube, Cxx             CNY                             

9243650  Comprehen Valid., EZ1/QIAcube/QIAsymph    """The Comprehensive Validation Support plan specific to a laboratory＇s needs will incorporate all of the requirements for the internal validation guidelines from the FBI＇s Quality Assurance Standards (QAS).

The Comprehensive Validation Support service includes all travel ａnd labor charges for up to 20 days by the QIAGEN Validation Team. Typically, one week will be required to extract, quantify, ａnd amplify the validation study samples ａnd may require multiple individuals. Usually, two weeks is required to analyze the data ａnd one week to write the final report. The expected time period to complete the Comprehensive Validation is six weeks. The actual time may vary depending on resource availability, including laboratory time dedicated to validation activities.

Lab Work – The QIAGEN specialist(s) will travel to the laboratory to conduct the on-site validation lab work. The QIAGEN specialist team will perform ａnd complete all extractions, quantifications, amplfications, ａnd capillary electrophoresis. The laboratory is responsible for providing systems/protocols that may be needed (generally laboratory SOPs, ex. interpretation guidelines). Additionally, the laboratory staff may be required to provide additional data needed for interpretation (ex. quantification/amplification/CE results) during the data review phase of the validation.

Once the final scope of the validation is determined, QIAGEN will provide a quote for all consumables required for the validation.  Non-QIAGEN chemistry ａnd accessories are not included ａnd should  be purchased to cover the validation experimental design including qPCR, STR, NIST standards, required samples types (blood, semen, buccal samples) ａnd required substrates (swabs, materials, etc).   The Customer is expected to have all materials on-hand 1 week prior to the validation start date.

Data Analysis – The Comprehensive Validation Support plan encompasses data collection (quants, amps, CE) during the on-site visit. The laboratory may be responsible for sharing (typically electronically via e-mail) any validation data requested after the on-site visit. A QIAGEN specialist will complete all of the data analysis produced from the validation study design. This includes qPCR (quant) ａnd STR (amp) analysis ａnd linkage to starting samples.

Validation data CD/flashdrive – QIAGEN will review the validation data ａnd draft a validation summary document, ensuring compliance with the FBI＇s QAS requirements/standards. QIAGEN will send validation data in an electronic format (email/CD/flashdrive) to the laboratory. The laboratory is responsible for final compilation, any format change preferences, review ａnd signoff of the validation report. The documentation provided by QIAGEN will aide in demonstrating compliance with all accreditation guidelines during laboratory audits. Should a hard copy of the validation study be preferred, the laboratory is required to specify prior to the onset of the validation.

Post-validation QIAGEN-to-Laboratory Transfer – Following completion of the validation ａnd generation of the draft validation summary document, a designated member of the QIAGEN validation team will review the validation with the Lab Manager ａnd the Technical Leader. Following sign off on the validation report, training of the laboratory DNA staff will occur. Refer to the Post-validation Competency Training for additional information. """     CNY                             

9237333  Install, BR plus 3 integr. w. scheduling     Installation ａnd training, BioRobot System including three approved integration devices with scheduling   CNY                             

9237328  Upgrade, BR & 2 ext. devices w. sched.  Upgrade BioRobot with two approved integration devices using scheduling software  CNY                             

9237173  Shield, V-housing               CNY                             

9233208  Tubing, liquid system 2.5/1.5             CNY                             

9237155  V-Housing with Sample Needle                  CNY                             

9237150  Heater Top                CNY                              【兔子巨噬细胞炎性蛋白1α(MIP-1α/CCL3)ELISA试剂盒现货】

9016151  #App. Package, M48, Pathology               CNY                             

9019179  Opto - plate lock closed, PyroQ96MD               CNY                             

9011883  Upgrade, high speed pipetting system             CNY                             

9011882  Back unit, BR9600            CNY                             

9011823  Z-Modul; Tip-Adapter 300µl; BR8000               CNY                             

9236916  3 Year Warranty, LiquiChip       LiquiChip 3-year Warranty with 1 Preventive Maintenance per year.  Included in system price.  This warranty must be included as a line item on the system quote.  It covers the system for three years total including all per incident travel ａnd labor charges, all repair parts, one preventive maintenance visits per year (three in total), ａnd 48- hour priority response.     CNY                             

9019143  Power supply, PyroQ96             CNY                             

9019129  Cable - Reagent, PyroQ96ID             CNY                             

9019126  PCB 2 - Connection, PyroQ96ID                 CNY                             

9019114  IPC mainboard, PyroQ96ID               CNY                             

9019025  Worktable, side wall, left, EZ1xl                CNY                             

9019012  Motor Y, EZ1xl          CNY                             

9018942  Adapter, 2xRing of 12 COBAS Amplicore          CNY                             

9022737  Rotor-Gene AssayManager SOW              CNY                             

9016061  Tubing Set, Syringe inlet, BR8000 I           CNY                             

QY-L0801 水杨酸69-72-7        Salicylic acid     69-72-7    20mg                          

QY-L0802 石杉碱甲102518-79-6   (-)-Huperzine A         102518-79-6    20mg                          

QY-L0803 石杉碱乙（95%）103548-82-9      Huperzine B     103548-82-9    20mg                          

QY-L0804 3,4,5-三甲氧基苯甲酸甲酯1916-07-0  Methyl 3,4,5-trimethoxybenzoate  1916-07-0         20mg                         

QY-L0805 3,4,5-三甲氧基苯甲酸118-41-2    Gallic acid trimethyl ether       118-41-2  20mg                          

QY-L0806 石斛碱2115-91-5   Dendrobine      2115-91-5         20mg                          

QY-L0807 水飞蓟宾22888-70-6      Silibinin     22888-70-6       20mg                          

QY-L0808 异水飞蓟宾72581-71-6 Isosilybin  72581-71-6       20mg                          

QY-L0809 石吊兰素152743-19-6   Lysionotin          152743-19-6    20mg                          

QY-L0810 麝香酮541-91-3      3-Methylcyclopentadeca          541-91-3  20mg                          

QY-L0811 酮麝香81-14-1        "Musk ketone

"       81-14-1    20mg                           【兔子巨噬细胞炎性蛋白1α(MIP-1α/CCL3)ELISA试剂盒现货】

QY-L0812 桑辛素62596-29-6 Morusin   62596-29-6       20mg                          

QY-L0813 桑色素480-16-0      Morin       480-16-0  20mg                          

QY-L0814 桑皮苷A102841-42-9     mulberroside A         102841-42-9    20mg                          

QY-L0815 桑皮苷C102841-43-0      Mulberroside C         102841-43-0    20mg                          

QY-L0816 桑根酮 C 80651-76-9      Sanggenon C    80651-76-9       20mg                          

QY-L0817 桑根酮 D  81422-93-7 Sanggenon D   81422-93-7       20mg                          

QY-L0818 D-松醇10284-63-6 D-Pinitol   10284-63-6       20mg                          

QY-L0819 山姜素36052-37-6 Alpinetin          36052-37-6       20mg                          

QY-L0820 水晶兰苷5945-50-6        Monotropein   5945-50-6         20mg                          

QY-L0821 鼠李糖6155-35-7   L-Rhamnose monohydrate       6155-35-7         20mg                          

QY-L0822 穗花杉双黄酮1617-53-4        Amentoflavone         1617-53-4         20mg                          

QY-L0823 斯皮诺素72063-39-9      Spinosin    72063-39-9       20mg                          

QY-L0824 酸枣仁皂苷A55466-04-1        Jujuboside A     55466-04-1       20mg                          

QY-L0825 酸枣仁皂苷B55466-05-2        Jujuboside B     55466-05-2       20mg                          

QY-L0826 酸枣仁皂苷A1194851-84-8   Jujuboside A1   194851-84-8    20mg                          

QY-L0827 酸枣仁皂苷B168144-21-8     Jujuboside B1   68144-21-8       20mg                           【兔子巨噬细胞炎性蛋白1α(MIP-1α/CCL3)ELISA试剂盒现货】

QY-L0828 松脂醇二葡萄糖苷63902-38-5      Pinoresinol diglucoside     63902-38-5       20mg                          

QY-L0829 桃叶珊瑚苷479-98-1      Aucubin    479-98-1  20mg                          

QY-L0830 商陆皂苷甲65497-07-6 Esculentoside A        65497-07-6       20mg