1.购买我公司任意ELISA试剂盒指标享受免费代测，并提供技术指导服务！

二、实验材料与试剂配制：

1. 仪器与材料：酶标仪（使用前预热30分钟），微量加液器、吸头、蒸馏水或去离子水，滤纸。

2. 缓冲液使用：加5ul  的缓冲液于50ul  的样品中，如果样品量不够或者不确定，缓冲液和样品的混合比例不要小于1:10即可混匀，静置1小时备用（如果标本是血清或者血浆，此步骤忽略）。

3. 洗液的配制：按1:100的比例配制洗液备用。

齐一生物销售：021－6034 8496；181214 53965；173021 04490

【猪热休克蛋白90(HSP-90)ELISA 试剂盒现货】三. 仔细阅读说明书

1.检查试剂盒标签上的有效期。如果超过有效期，请勿使用。

2. 按说明书确定所有试剂齐全（数量、体积）

3. 标本制备要规范，每份标本体积要按2-3个复孔以上的量制备（贮存），尽量分装做备份。

4.短期内无法实验者，注意低温保存

5.准备好所有实验额外所需物品，（比如移液器，试管，清洗器，酶标仪）

6. 按说明书将所用试剂平衡至室温，根据检测标本数量确定所需试剂的量

7.检查试剂盒内每种试剂的贮存条件，保证所有试剂均按照试剂盒内说明书推荐的条件存储。 【猪热休克蛋白90(HSP-90)ELISA 试剂盒现货】

8.检查不稳定或者变质的试剂溶液（比如沉淀或变色）。有些试剂，比如标准品稀释液或者检测稀释液，可能在设计时就含有沉淀物。所有试剂在滴入酶标板之前，必需充分混匀。而由于沉淀物的特性，在加入酶标板之前，必需不停搅拌。

9.保证充分的孵育时间和温度。

10.切勿使用不同生产批号的试剂取代现有试剂或混合现有试剂。

11.在混合或溶解蛋白溶液时，避免泡沫产生。

12.在实验开始之前，安排好实验流程。在实验开始之前，清洁工作台。

13.若有问题，应与我公司或代理商的技术支持联系

【猪热休克蛋白90(HSP-90)ELISA 试剂盒现货】四、客户须知

齐一生物销售：021－6034 8496；181214 53965；173021 04490

1.在收集标本前都必须有一个完整的计划，必须清楚要检测的成份是否足够稳定。我们提倡新鲜标本尽早检测，对收集后当天就进行检测的标本，及时储存在4℃备用，如有特殊原因需要周期收集标本，请造模取材后，将标本及时分装后放在-20℃或-70℃条件下保存。因冰室与室温存在一定温差，蛋白极易降解，直接影响实验质量，所以避免反复冻融。部分标本也可加入适当防腐剂，激素类标本需添加抑肽酶。 

2.试剂盒代测标本的客户取材前须向我司销售人员索要说明书，具体操作注意事项请与我司技术人员沟通。

3.液体类标本：标本必须为液体，不含沉淀。包括血清、血浆、尿液、胸腹水、脑脊液、细胞培养上清、组织匀浆等。

4.购买前请提前告诉我们：您样本的种属、样本数量、待测指标及其它特殊要求。

【猪热休克蛋白90(HSP-90)ELISA 试剂盒现货】五、代测数据详细内容

1、ELISA标准曲线；

2、实验具体详细步骤；

3、完整的实验报告（试剂耗材，实验方法，实验结果及结果说明）；

4、实验进行阶段，我公司客服人员会及时向您汇报实验进程。

9013394  Tubing set , HSPS, 200               CNY                              【猪热休克蛋白90(HSP-90)ELISA 试剂盒现货】

9013235  Tubing set,  90/120, vacuum, 0               CNY                             

9013226  Gripper set, L-handler, BR3000                 CNY                             

9013199  Tub.pre.sen.inlet,auto vac,BR8000 v1.00                 CNY                             

9011774  Tip Adapter 1100 ul BR8000             CNY                             

9011709  Dispenser head, BR8000          CNY                             

132559     NeXtal Stock Magnesium acetate (50)    1M Magnesium acetate tetrahydrate - 50mL (4 x 12,5 ml tube)    CNY                            

800309     siRNA Hairpin Run w/o prep             CNY                             

800160     Single Read, w/o prep      "Sequencing of plasmid DNA ａnd PCR products, including DNA quality control

Repetition of sequencing, if necessary

Data collected to maximal read length" CNY                             

800101     Basic Read Tubes               CNY                             

765512     PAXgene Tissue STABILIZER Conc. (150 ml)              CNY                             

132007     EasyXtal 15-well Tool (100)                CNY                             

201207     Taq DNA Polymerase (5000)    "20 x 250 units Taq DNA Polymerase, 10x PCR Buffer,* 5x Q-Solution, 25 mM MgCl2

NEW! Now with 10x CoralLoad PCR Buffer

Taq DNA Polymerase is now supplied with CoralLoad PCR Buffer, which has all of the advantages of QIAGEN PCR Buffer but can also be used to directly load the PCR reaction onto an agarose gel without the need to add a gel loading buffer. CoralLoad PCR Buffer provides the same high PCR specificity ａnd minimal reaction optimization as conventional QIAGEN PCR Buffer. Additionally, it contains two marker dyes: an orange dye ａnd a red dye, that facilitate estimation of DNA migration distance ａnd optimization of agarose gel run time."         CNY                             

9238319  V-drive unit, BR8000, V2          CNY                             

9238178  QIAplate Holder, SBS, black              CNY                             

9238134  Multiwell-Plate Holder, SBS              CNY                             

9240675  Tee, Barbed, Plastic, AP96                CNY                              【猪热休克蛋白90(HSP-90)ELISA 试剂盒现货】

9240653  Pressure Pump, Gast, 120V, AP96            CNY                             

9240624  Solid State Relay, AP196/98               CNY                             

9240576  Cable Assy, Capper, X51,CCW, AP96/196           CNY                             

9240546  Motor, Shutter Door, Eppendorf, AP196/98               CNY                             

9240544  Centrifuge, 5810RA, Eppendorf, AP196/98                CNY                             

9240513  Mouse, Standard, AP                 CNY                             

9240508  Software & Std. Protocols, AP98               CNY                             

9240499  Reagent Pump, AP            CNY                             

9240378  Autopure, Installation      The Installation, AUTOPURE facilitates the quick implementation of the AUTOPURE into the lab routine by minimizing the instrument start-up phase. It covers the hardware ａnd software installation of the AUTPOURE instrument ａnd provides the user with an introductory training on operation ａnd user maintenance. The installation includes labor ａnd travel costs. The performance of the installation process is documented on a GMP/GLP-compliant Field Service document.         CNY                             

9240242  Connector, disp. tip adapter drive, TR              CNY                             

9239959  EZ1, Premium Agreement       The Premium Cover Agreement EZ1 provides full service coverage for the BioRobot EZ1. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visits, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 24 hours (1 working day).         CNY                             

9020028  ZB sensor, 320mm, EZ1xl                  CNY                             

9020018  Upgrade kit, vacuum chamber, CAS-1820                 CNY                             

9019987  PCB, small breakout, 8 core, Cxx               CNY                             

9019927  Cover, tip ejector (UV), Cx8               CNY                             

QY-x2449 人宫颈癌肠转移细胞 Ca Ski 询价 ATCC                                                  

QY-x2450 大鼠胶质瘤细胞 C6 询价 ATCC                                                         【猪热休克蛋白90(HSP-90)ELISA 试剂盒现货】

QY-x2451 人乳腺管癌细胞 BT-549 询价 ATCC                                                

QY-x2452 仓鼠幼仓鼠肾细胞 BHK-21 [C-13] 询价 ATCC                                                 

QY-x2453 人肝癌细胞 BEL-7402 询价 ATCC                                                  

QY-x2454 小鼠黑色素瘤细胞 B16 询价 ATCC                                                 

QY-x2455 人转移胰腺腺癌细胞 AsPC-1 询价 ATCC                                                

QY-x2456 人胚肾细胞 AAV-293 询价 ATCC                                                      

QY-x2457 人肺癌细胞 A549 [A-549] 询价 ATCC                                                       

QY-x2458 人表皮癌细胞 A-431 询价 ATCC                                                      

QY-x2459 人T淋巴细胞白血病细胞 A3 询价 ATCC                                                        

QY-x2460 人高转移肺癌细胞 95-D 询价 ATCC                                                        

QY-x2461 人肾透明细胞腺癌细胞 786-O 询价 ATCC                                                      

QY-x2462 小鼠脂肪细胞 3T3-L1 询价 ATCC                                                     

QY-x2463 人胚肾细胞 293T 询价 ATCC                                                   

QY-x2464 人膀胱癌细胞 5637 询价 ATCC                                                        

QY-x2465 人胚肾细胞 293 询价 ATCC                                                      

QYS-03001        辅酶ⅠNADH含量测试盒      50管/48样      高效液相色谱法                                 【猪热休克蛋白90(HSP-90)ELISA 试剂盒现货】

QYS-03002        辅酶ⅠNADH含量测试盒      50管/48样      可见分光光度法                                

QYS-03003        辅酶ⅠNADH含量测试盒      100管/96样    酶标法                                

QYS-03004        NAD激酶NADK测试盒 50管/24样      可见分光光度法                                

QYS-03005        乳酸脱氢酶LDH测试盒         50管/24样      可见分光光度法                                

QYS-03006        乳酸脱氢酶LDH测试盒         100管/48样    酶标法                                

QYS-03007        苹果酸脱氢酶MDH测试盒  50管/48样      紫外分光光度法                                

QYS-03008        线粒体呼吸链复合体Ⅰ活性测试盒     25管/24样      紫外分光光度法                                

QYS-03009        NADH氧化酶NOX测试盒     50管/48样      可见分光光度法                                

QYS-03010        柠檬酸合酶CS测试盒   50管/48样      可见分光光度法                                

QYS-03011        柠檬酸合酶CS测试盒   25管/24样      可见分光光度法                                

QYS-03012        柠檬酸合酶CS测试盒   10管/9样         可见分光光度法                                

QYS-03013        丙酮酸脱羧酶PDC测试盒    50管/48样      紫外分光光度法                                

QYS-03014        醇脱氢酶ADH测试盒   50管/48样      紫外分光光度法                                

QYS-03015        单脱氢抗坏血酸还原酶MDHAR测试盒      50管/48样      紫外分光光度法                                

QYS-03016        辅酶ⅡNADPH含量测试盒    50管/48样      高效液相色谱法