1. 酶标仪(450nm 波长滤光片)

2. 高精度移液器，EP 管及一次性吸头

3. 37℃恒温箱，双蒸水或去离子水

4. 吸水纸

【猪皮质酮/肾上腺酮(CORT)ELISA 试剂盒厂家】齐一生物【友情提示】：本产品仅供科研研究使用，不得用于人体临床直接检测。避免给您带来不必要的损失，请仔细阅读购买说明！

ELISA应用：

ELISA检测试剂盒、ELISAKit、酶联免疫试剂盒、酶联免疫吸附测定试剂盒、酶联免疫分析试剂盒、酶免试剂盒等,比较常见的叫法是ELISA检测试剂盒、酶联免疫吸附测定试剂盒等ELISA试剂盒自从60-70年代问世以来,得到全世界科研工作者的认可及推崇,在欧美及中国获得很大的推广,尤其是国内生化领域的长足发展.Elisa生物试验是一种敏感性高,特异性强,重复性好的实验诊断方法.由于其试剂稳定、易保存,操作简便,结果判断较客观等因素,已广泛应用在免疫学检验的各领域中.ELISA检测试剂盒适用于体外定性检测人血清或血浆中的抗人类戊型肝炎（HEV）病毒IgM抗体ELISA检测

【猪皮质酮/肾上腺酮(CORT)ELISA 试剂盒厂家】齐一生物科技欢迎您订购咨询，齐一生物销售：021－6034 8496；181214 53965；173021 04490

齐一生物供应的ELISA试剂盒安全性提示：

1.工作人员实验中避免直接接触终止液和底物A、B。一旦接触到这些液体，请尽快用水冲洗。

2.实验中不要吃喝、抽烟或使用化妆品。

3.不要用嘴吸取试剂盒里的任何成份。

样本保存条件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6个月

3.-50℃以下的冰箱，可以放一年

【猪皮质酮/肾上腺酮(CORT)ELISA 试剂盒厂家】齐一生物供应的ELISA试剂盒ELISA实验原理

试剂盒是固相夹心法酶联免疫吸附实验（ELISA）.已知浓度的标准品、未知浓度的样品加入微孔酶标板内进行检测.先将生物素标记的抗体同时温育.洗涤后,加入亲和素标记过的HRP.再经过温育和洗涤,去除未结合的酶结合物,然后加入底物A、B,和酶结合物同时作用.产生颜色.颜色的深浅和样品中的浓度呈比例关系.

免疫测定技术的基础在于抗原抗体之间的特异结合反应,所以任何优质的诊断试剂离不开优质的原料,如抗原抗体,酶等.以前用于免疫测定的抗原通常为各种纯化抗原,而抗体则为纯化抗原免疫动物后获得的多克隆抗体和使用杂交瘤技术得到的单克隆抗体,而抗原或抗体的标记物如酶标结合物则通过各种化学合成方法制备.近年来,随着分子生物学的发展,使用基因工程方法制备各种特殊的抗原或抗体及其酶结合物等免疫测定试剂几成为现实的新一代试剂,各种新型使用的测定方法也不断出现.

HBsAg试剂特点（检测模式：临床抗体夹心法）：包被抗体为山羊多抗.多抗具有高亲和性和对抗原各种表位的反应性.酶表抗体为与HBsAg有不同结合位点的鼠复合单位,可测得HBsAg变异样品,提高检测的特异性（99.98%）提高检测的灵敏度,应用ParlEhrich学说（PEI）HBsAg标准品,对ad标准品的灵敏度为0.05ng/ml对ay标准品灵敏度为0.025ng/ml

ELISA检测试剂盒应用定性夹心免疫检测技术,用合成的HEV多肽抗原包被微孔板板条,这些多肽是中国型HEV毒株核心氨基酸序列中抗原性很强的肽段,分别来自于该毒株的开放阅读框2和开放阅读框3.将样品或标准品加入孔中并孵育,如果其中存在HEVIgM抗体,这些抗体就会与HEV的多肽抗原结合,并固定在上面,洗板除去其它非特异性抗体和样品中的其它成份.然后加入羊抗人IgM-HRP（辣根过氧化物酶）酶结合物,经第二次孵育后,酶结合物就会与次孵育结合上的HEVIgM抗体相结合,洗板除去未结合的酶结合物,加入TMB底物溶液,在第三次孵育时会发生酶-底物反应,只有那些含有HEVIgM抗体和酶结合物所形成的复合物的孔才会发生颜色变化,加入硫酸溶液终止酶和底物间的反应,并在波长:450nm处测量O.D.值,按照本HEVIgM抗体elisa试剂盒的测试标准,O.D.值大于或等于Cut-Off值的样品被认为是初试阳性. 【猪皮质酮/肾上腺酮(CORT)ELISA 试剂盒厂家】

以上资料仅供参考了解，不供于论文写作

齐一生物专业为您提供高品质，高灵敏度.高质量的酶联免疫试剂盒，齐一生物长期经营ELISA试剂盒（免费代测）及抗体、分子学生物试剂盒、金标法检测试剂盒、比色法检测试剂盒、细胞菌株、培养基、细胞因子、生化试剂、耗材等生物试剂产品。齐一生物销售：021－6034 8496；181214 53965；173021 04490

9012643  Bottle, system liquid                  CNY                              【猪皮质酮/肾上腺酮(CORT)ELISA 试剂盒厂家】

9012596  Disposable Tips, 300µl (960)    Conducting disposable tips       CNY                             

133121     NeXtalStock Lithium citrate  (200)                  CNY                             

133119     NeXtalStock Ammonium citrate  (200)           CNY                             

133190     NeXtalStock D-(+)-Glucose (200)               CNY                             

51162       QIAamp 96 DNA Blood Kit (12)        For 12 x 96 DNA preps: 12 QIAamp 96 Plates, QIAGEN Protease, Reagents, Buffers, Lysis Blocks, Tape Pads, Collection Vessels       CNY                             

50112       QIAamp UCP PurePathogen Blood Kit (10)               CNY                             

133104     NeXtalStock Sodium acet.  (200)            CNY                             

9015468  Sensor, photo, PM-L24              CNY                             

9001871  EZ1 Advanced, System     EZ1 Advanced, System combines the EZ1 Advanced system ａnd installation. The Installation ａnd Introductory Training is an on-site service, conducted by a QIAGEN Service Specialist. It provides the user with a training on operation ａnd user maintenance. All travel ａnd labor cost included for Installation The installation includes labor ａnd travel costs. The performance of the installation process is documented on a GMP/GLP-compliant Field Service document. CNY                             

9018458  Screw, w finishing washer (4), QX             CNY                             

9018447  Purge Tool, cartridge, QX                  CNY                             

9012412  Incubator Tower (65°) for Plato 3 hold              CNY                             

9012392  Bottle, waste, 10 l, BR8000              CNY                             

205111     Omniscript RT Kit (50)      For 50 reverse-transcription reactions: 200 units Omniscript Reverse Transcriptase, 10x Buffer RT, dNTP Mix,* RNase-free water         CNY                             

9018425  Door, sample, QX              CNY                             

9002123  QIAxcel Advanced System        QIAxcel Advanced System combines the QIAxcel Advanced instrument ａnd installation. The Installation ａnd Introductory Training is an on-site service, conducted by a QIAGEN Service Specialist. It provides the user with training on operation ａnd user maintenance. All travel ａnd labor cost included for Installation. Performance of the installation process is documented on a GMP/GLP-compliant Field Service document.          CNY                              【猪皮质酮/肾上腺酮(CORT)ELISA 试剂盒厂家】

57714       QIAamp MinElute Virus Vacuum Kit (50)         For 50 minipreps: 50 QIAamp MinElute Columns, QIAGEN Protease, Carrier RNA, Buffers, Extension Tubes (3 ml), Collection Tubes (1.5 ml)      CNY                             

972012     PyroMark Q96 CpG p16 (96)    For quantification of methylation level in p16 CNY                             

133227     NeXtalStock Sodium cacodyl. pH 6.0 (100)                CNY                             

301108     PolyFect Transfection Reagent (100 ml)  For 2500-6500 transfections in 60 mm dishes or 5000-10,000 transfections in 6-well plates.      CNY                             

56304       QIAamp DNA Micro Kit (50)    For 50 DNA preps: 50 QIAamp MinElute Columns, Proteinase K, Carrier RNA, Buffers, Collection Tubes (2 ml)     CNY                             

9002031  Rotor-Gene Q MDx 5Plex HRM no PC               CNY                             

9002018  Rotor-Gene Q MDx 2plex HRM, Priority Rotor-Gene Q 2plex HRM, Priority includes the Rotor-Gene Q system, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with on site introductory training. Priority package extends warranty period of the system to 2 years with shipment to Repair Center Service has response turnaround time (repair) 7-10 days. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Replacement system (Loaner) provision included (provision time 2-3 days) with transportation cost coverage. The service package includes all parts, labor ａnd travel costs with installation performance ａnd service visits documented on a GMP/GLP-compliant Field Service documents.        CNY                             

970902     PyroMark Q24 Advanced Kit (4x24)                   CNY                             

133211     NeXtalStock Magnesium chloride H2O (200)            CNY                             

133195     NeXtalStock Sodium tartrate pH 5.6 (200)                CNY                             

293023     mericon Corn (24)             CNY                             

292055     mericon Goat Kit (96)                CNY                             

929521     QX Alignment Marker 15 bp/1 kb (1.5 ml)                 CNY                             

9012598  Disposable Filter-Tips, 1100µl (960)         Conducting disposable filter-tips      CNY                             

206152     QIAGEN Multiplex PCR Plus Kit (100)                CNY                             

QKT0930  水生动物病毒基因组DNA提取试剂盒                                                      

QKT0931  禽流感病毒核酸提取试剂盒                                                       

QKT0932  水生动物病毒基因组DNA/RNA提取试剂盒                                                     

QKT0933  深加工食品DNA提取试剂盒                                                      

QKT0934  动物组织基因组DNA快速提取试剂盒                                                      

QKT0935  细菌基因组DNA快速提取试剂盒                                                      

QKT0936  结核分枝杆菌复合群核酸检测试剂盒扩增法                                                  

QKT0937  军团菌属核酸检测试剂盒荧光法                                                       

QKT0938  嗜肺军团菌核酸检测试剂盒荧光法                                                   【猪皮质酮/肾上腺酮(CORT)ELISA 试剂盒厂家】

QKT0939  登革热病毒DENV I型病毒核酸检测试剂盒荧光法                                                 

QKT0940  登革热病毒DENV II型病毒核酸检测试剂盒荧光法                                                

QKT0941  登革热病毒DENV III型病毒核酸检测试剂盒荧光法                                              

QKT0942  羊源性成分核酸检测试剂盒PCR-荧光探针法                                                  

QKT0943  登革热病毒DENV IV型病毒核酸检测试剂盒荧光法                                              

QKT0944  呼吸道21种病原体荧光检测试剂盒                                                

QKT0945  强化型呼吸系统病原体荧光检测试剂盒                                                  

QKT0947  细菌性肺炎荧光检测试剂盒                                                       

QKT0948  呼吸系统33种病原体荧光检测试剂盒                                                     

QKT0950  博德氏菌荧光检测试剂盒                                                  

QKT0951  流感荧光检测试剂盒                                                  

QKT0952  ACE荧光检测试剂盒                                                   

QKT0953  巨细胞病毒荧光检测试剂盒                                                       

QKT0954  病毒性肠胃炎荧光检测试剂盒                                                  

QKT0955  细菌性肠胃炎荧光检测试剂盒                                                  

QKT0956  肠内寄生虫荧光检测试剂盒                                                        【猪皮质酮/肾上腺酮(CORT)ELISA 试剂盒厂家】

QKT0957  消化系统荧光检测试剂盒                                                  

QKT0958  艰难梭状杆菌荧光检测试剂盒                                                  

QKT0959  丙肝病毒荧光检测试剂盒                                                  

QKT0960  丙肝病毒荧荧光检测试剂盒                                                       

QKT0961  疱疹病毒荧光检测试剂盒                                                  

QKT0962  病毒性脑膜炎荧光检测试剂盒                                                  

QKT0963  细菌性脑膜炎荧光检测试剂盒                                                  

QKT0964  腮腺炎荧光检测试剂盒