【友情提示】：本产品仅供科研研究使用，不得用于人体临床直接检测。避免给您带来不必要的损失，请仔细阅读购买说明！

本试剂盒仅供研究使用，齐一生物销售：021－6034 8496；181214 53965；173021 04490欢迎选购！

操作步骤

1.准备试剂，样品和标准品

2.加入准备好的样品和标准品，37℃反应30分钟

3.洗板5次，加入酶标试剂，37℃反应30分钟

4.洗板5次，加入显色液A、B，37℃显色15分钟

5.加入终止液

6.15分钟之内读OD值

7.计算
齐一生物供应ELISA试剂盒检测的目的是为实验提供准确可靠的定量分析实验依据。为了保证实验数据的可靠性，在实验过程中必须坚持全面质量控制和全过程质量控制。在收集标本前都必须有一个完整的计划。

【猪白介素1β (IL-1β)ELISA 试剂盒厂家】样本保存条件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6个月

3.-50℃以下的冰箱，可以放一年

【猪白介素1β (IL-1β)ELISA 试剂盒厂家】样本收集

1.血清样本用干燥管（红色）或者促凝管（黄色）收集

2.血浆用抗凝管，一般有EDTA抗凝（紫色）、枸橼酸钠抗凝（黑色或者蓝色）

3.动物组织匀浆（细胞）：

              1g组织加入9ml PBS（浓度为0.1M，pH为7.4的）测分泌型指标

              1g组织加入1ml组织裂解液加入8ml PBS 测细胞内

粪便样本：1g样本+9ml PBS（浓度为0.01M，pH为7.4的）

没有PBS的话也可以用一下生理盐水，还是用PBS

【猪白介素1β (IL-1β)ELISA 试剂盒厂家】样本离心转速及时间

1.试管采集的样本：3000转，离心五分钟取上清

2.离心管或者EP管采集的样本：5000－－8000转3分钟取上清

3..样本保存－－如果样品不立即使用，应将其分成小部分-70 ℃保存，避免反复冷冻。尽可能的不要使用溶血或高血脂血。如果血清中大量颗粒，检测前先离心或过滤。不要在37℃或更高的温度加热解冻。应在室温下解冻并确保样品均匀地充分解冻。

【猪白介素1β (IL-1β)ELISA 试剂盒厂家】以上样本采集及处理方式供您参考，如有问题请来电，齐一生物销售：021－6034 8496；181214 53965；173021 04490

800141    Investigation grade sequencing, w/ prep       "Single-stranded sequencing of an unknown sequence

QIAGEN DNA template preparation

Design ａnd synthesis of walking primers

Data assembly ａnd sequence alignment

Accuracy >99%"    CNY                            【猪白介素1β (IL-1β)ELISA 试剂盒厂家】

767134    PAXgene Tissue DNA Kit (50)             CNY                           

929605    QX Mineral Oil (50 ml)         CNY                           

330513    RT² SYBR Green Fluor qPCR Mastermix (24)            CNY                           

330502    RT² SYBR Green qPCR Mastermix (12)              CNY                           

330401    RT² First Strand Kit (12)              CNY                           

9010813  BGR Stirrer PK80          CNY                           

69506     DNeasy Blood & Tissue Kit (250) 250 DNeasy Spin Columns, QIAGEN Proteinase K, Buffers, Collection Tubes (2 ml)       CNY                           

63204     DyeEx 2.0 Spin Kit (50) 50 DyeEx Spin Columns, Collection Tubes (2 ml)       CNY                           

211392    Internal Control DNA (High conc.)              CNY                           

9012755  Set, Tubing, Washer, P3        CNY                           

9010126  Washer boat           CNY                           

979015    MOTT 16S Primer 1 for Sequencing           CNY                           

979010    PyroMark Vacuum Prep Filter Probe (100)   Re-usable filter probes for PyroMark Vacuum Workstation Q96 ａnd Q24.       CNY                           

59720     EpiTect Fast 96 DNA Bisulfite Kit         CNY                           

9002245  QIAcube(220-240v) Promo, PrioPLUS QIAcube (220-240V) Promo Priority includes the QIAcube instrument +promo iPad, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with introductory training. Priority package extends warranty period of the system to 3 years. One preventive maintenance visit is included for each year of the warranty; 3 preventive maintenance visits in total. Service package includes all parts, labor ａnd travel costs with a service response time of 48 hours (2 working days). Performance of the installation ａnd service visits are documented on a GMP/GLP-compliant Field Service documents.   CNY                           

9002198  RGQ 5plex HRM, PC, PrioPLUS, no PM     "RGQ 5plex, HRM, PC Instrument

Installation of RGQ 5plex HRM & Introductory training

Warranty extension to 3 yrs

Repair Center service, response 7-10 days

Loaner available in 2 days*

No preventive Maintenance

Priority service provision over non-contract customers

Documented installation & repair report

Unlimited number of repair service

All cost covered (Shipping, labor, parts)"     CNY                            【猪白介素1β (IL-1β)ELISA 试剂盒厂家】

9002197  RGQ 5plex, PC, PrioPLUS, basic, no PM    "RGQ 5plex, PC Instrument

Installation of RGQ 2plex & Introductory training

Warranty extension to 3 yrs

Repair Center service, response 7-10 days

No Loaner available

No Preventive Maintenance

Priority service provision over non-contract customers

Documented installation & repair report

Unlimited number of repair service

All cost covered (Shipping, labor, parts)"     CNY                           

9012718  Pipetting Position (raised platform)              CNY                           

9002042  Rotor-Gene Q MDx 6plex Platform             CNY                           

9002038  Rotor-Gene Q MDx 5plex HRM, Priority     Rotor-Gene Q MDx 5plex HRM, Priority includes the Rotor-Gene Q system, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with on site introductory training. Priority package extends warranty period of the system to 2 years with shipment to Repair Center Service has response turnaround time (repair) 7-10 days. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Replacement system (Loaner) provision included (provision time 2-3 days) with transportation cost coverage. The service package includes all parts, labor ａnd travel costs with installation performance ａnd service visits documented on a GMP/GLP-compliant Field Service documents. CNY                           

59110      EpiTect 96-well Bisulfite Kit (2)    2x EpiTect Bisulfite 96-well Plates, Reaction Mix, DNA Protect Buffer, Carrier RNA, Buffers    CNY                           

133225    NeXtalStock Sodium ac. pH 4.8 (200)         CNY                           

301305    SuperFect Transfection Reagent (1.2 ml)     For 40 transfections in 60 mm dishes or 160 transfections in 12-well plates       CNY                           

970802    PyroMark Gold Q24 Reagents (5 x 24) For performing pyrosequncing reaction on the Q24     CNY                           

970470    BRAF Pyro Kit (24)              CNY                           

970061    MGMT Pyro Kit (48)            CNY                           

133209    NeXtalStock MES pH 5.0 (100)           CNY                           

292065    mericon Sheep Kit (96)         CNY                           

292045    mericon Turkey (96)            CNY                           

929501    QX Pro Calibration Marker (0.6 ml)            CNY                           

9012658  Cable, X-BCR board to buffer track reade           CNY                           

QYD115  猪囊虫抗体检测试剂盒       96孔/盒 评估猪场猪瘟疫苗免疫状况，感染猪的血清学诊断                           

QYD116  猪轮状病毒IgM抗体（RV-IgM)检测试剂盒     97孔/盒 用于测定猪血清、血浆及相关液体样本中轮状病毒IgM 抗体（RV-IgM)表达                      

QYD117  猪大肠杆菌K99 检测试剂盒      96孔/盒 用于测定猪血清、血浆及相关液体样本中大肠杆菌K99表达                            【猪白介素1β (IL-1β)ELISA 试剂盒厂家】

QYD118  猪大肠杆菌987P 检测试剂盒    96孔/盒 用于测定猪血清、血浆及相关液体样本中大肠杆菌987P表达                         

QYD119  猪大肠杆菌K88 检测试剂盒     96孔/盒 用于测定猪血清、血浆及相关液体样本中大肠杆菌K88表达                           

QYD120  猪传染性肠胃炎病毒检测试剂盒       96孔/盒 用于测定猪血清、血浆及相关液体样本中传染性胃肠炎病毒(TGEV)表达                           

QYD121  猪流行性腹泻病毒（PEDV）检测试剂盒        96孔/盒 用于测定猪血清、血浆及相关液体样本中流行性腹泻病毒（PEDV）表达                         

QYD122  猪轮状病毒抗原（RV-Ag)检测试剂盒       96孔/盒 用于测定猪血清、血浆及相关液体样本中轮状病毒抗原（RV-Ag)表达                           

QYD123  猪圆环病毒抗原（PCV）检测试剂盒 96孔/盒 用于测定猪血清、血浆及相关液体样本中圆环病毒（PCV）表达                     

QYD124  猪链球菌（strep）检测试剂盒    96孔/盒 用于测定猪血清、血浆及相关液体样本中链球菌（strep）表达                        

QYD125  猪嗜血杆菌(HPS)检测试剂盒     96孔/盒 用于测定猪血清、血浆及相关液体样本中嗜血杆菌(HPS)表达                         

QYD126  鸡禽流感H5N1抗体检测试剂盒 96孔/盒                             

QYD201  鸡禽流感H9抗体检测试剂盒     96孔/盒                             

QYD201  鸡传染性喉气管炎抗体检测试剂盒    96孔/盒                             

QYD201  鸡法氏囊抗体检测试剂盒    96孔/盒                             

QYD201  鸡新城疫抗体检测试剂盒    96孔/盒                             

QYD301  狗狂犬抗体检测试剂盒       96孔/盒                             

QYD302  旋毛虫抗体检测试剂盒       96孔/盒                             

QYD303  犬恶心丝虫抗体检测试剂盒       96孔/盒                              【猪白介素1β (IL-1β)ELISA 试剂盒厂家】

QYD401  牛羊亚1型口蹄疫       96孔/盒                             

QYD402  猴B病毒抗体检测试剂盒(定量）      96孔/盒                             

QYD403  肺吸虫试剂盒       96孔/盒                             

QYD500  猪蓝耳抗体检测卡       50T/盒                               

QYD501  猪口蹄疫0型抗体检测卡    50T/盒                               

QYD502  猪伪狂犬抗体检测卡    50T/盒                               

QYD503  猪圆环抗体检测卡       50T/盒                               

QYD504  猪细小抗体检测卡       50T/盒                               

QYD505  猪乙脑抗体检测卡       50T/盒                               

QYD506  鸡禽流感抗体检测卡    50T/盒                               

QYD507  鸡禽流感抗原检测卡    50T/盒                               

QYD508  H7亚型禽流感抗原快速检测卡  50T/盒                               

QYD509  H5亚型禽流感抗原快速检测卡  50T/盒                               

QYD510  H9亚型禽流感抗原快速检测卡  50T/盒