检测标本：血清,血浆,尿液,胸腹水,脑脊液,细胞培养上清,组织匀浆等

检测方法：ELISA

检测类型：酶联免疫夹心法

产品的用途：仅供科研究课题使用

价格及详细资料：齐一生物销售：021－6034 8496；181214 53965；173021 04490；.齐一生物科技（上海）有限公司提供的ELISA试剂盒受到了广大科研单位的一致肯定和认同.大品牌保证,价格公道,倾力为国内外科研院校实验室提供*优质的产品.若有需要,我司将竭诚为您服务！本试剂盒用于测定血清,血浆及相关液体样本中含量.

【小鼠抗子宫内膜抗体(EMAb)ELISA试剂盒厂家】实验原理：

   本试剂盒应用双抗体夹心法测定标本中该产品水平.用纯化的本产品抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入本产品抗原,再与HRP标记的本产品抗体结合,形成抗体-抗原-酶标抗体复合物,经过彻底洗涤后加底物TMB显色.TMB在HRP酶的催化下转化成蓝色,并在酸的作用下转化成*终的黄色.颜色的深浅和样品中的本产品呈正相关.用酶标仪在450nm波长下测定吸光度（OD值）,通过标准曲线计算样品中该产品浓度.

试剂盒组成：

齐一生物销售：021－6034 8496；181214 53965；173021 04490

【小鼠抗子宫内膜抗体(EMAb)ELISA试剂盒厂家】标本要求：

1．标本采集后尽早进行提取,提取按相关文献进行,提取后应尽快进行实验.若不能马上进行试验,可将标本放于-20℃保存,但应避免反复冻融

2．不能检测含NaN3的样品,因NaN3抑制辣根过氧化物酶的（HRP）活性.

操作步骤：

1.     标准品的稀释与加样：在酶标包被板上设标准品孔10孔,在、第二孔中分别加标准品100μl,然后在、第二孔中加标准品稀释液50μl,混匀；然后从孔、第二孔中各取100μl分别加到第三孔和第四孔,再在第三、第四孔分别加标准品稀释液50μl,混匀；然后在第三孔和第四孔中先各取50μl弃掉,再各取50μl分别加到第五、第六孔中,再在第五、第六孔中分别加标准品稀释液50ul,混匀；混匀后从第五、第六孔中各取50μl分别加到第七、第八孔中,再在第七、第八孔中分别加标准品稀释液50μl,混匀后从第七、第八孔中分别取50μl加到第九、第十孔中,再在第九第十孔分别加标准品稀释液50μl,混匀后从第九第十孔中各取50μl弃掉.（稀释后各孔加样量都为50μl,浓度分别为1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L）. 【小鼠抗子宫内膜抗体(EMAb)ELISA试剂盒厂家】

2.     加样：分别设空白孔（空白对照孔不加样品及酶标试剂,其余各步操作相同）、待测样品孔.在酶标包被板上待测样品孔中先加样品稀释液40μl,然后再加待测样品10μl（样品*终稀释度为5倍）.加样将样品加于酶标板孔底部,尽量不触及孔壁,轻轻晃动混匀.

3.     温育：用封板膜封板后置37℃温育30分钟.

4.     配液：将30（48T的20倍）倍浓缩洗涤液用蒸馏水30（48T的20倍）倍稀释后备用.

5.     洗涤：小心揭掉封板膜,弃去液体,甩干,每孔加满洗涤液,静置30秒后弃去,如此重复5次,拍干.

6.     加酶：每孔加入酶标试剂50μl,空白孔除外.

7.     温育：操作同3.

8.     洗涤：操作同5.

9.     显色：每孔先加入显色剂A50μl,再加入显色剂B50μl,轻轻震荡混匀,37℃避光显色15分钟.

10. 终止：每孔加终止液50μl,终止反应（此时蓝色立转黄色）.

11. 测定：以空白空调零,450nm波长依序测量各孔的吸光度（OD值）. 测定应在加终止液后15分钟以内进行.

注意事项：

1． 试剂盒从冷藏环境中取出应在室温平衡15-30分钟后方可使用,酶标包被板开封后如未用完,板条应装入密封袋中保存.

2． 浓洗涤液可能会有结晶析出,稀释时可在水浴中加温助溶,洗涤时不影响结果. 【小鼠抗子宫内膜抗体(EMAb)ELISA试剂盒厂家】

3． 各步加样均应使用加样器,并经常校对其准确性,以避免试验误差.一次加样时间控制在5分钟内,如标本数量多,推荐使用排抢加样.

4． 请每次测定的同时做标准曲线,做复孔.如标本中待测物质含量过高（样本OD值大于标准品孔孔的OD值）,请先用样品稀释液稀释一定倍数（n倍）后再测定,计算时请*后乘以总稀释倍数（×n×5）.

5． 封板膜只限一次性使用,以避免交叉污染.

6． 底物请避光保存.

7． 严格按照说明书的操作进行,试验结果判定必须以酶标仪读数为准.

8． 所有样品,洗涤液和各种废弃物都应按传染物处理.

9． 本试剂不同批号组分不得混用.

10. 如与英文说明书有异,以英文说明书为准.

计算：

以标准物的浓度为横坐标,OD值为纵坐标,   

在坐标纸上绘出标准曲线,根据样品的OD     

值由标准曲线查出相应的浓度；再乘以稀释     

倍数；或用标准物的浓度与OD值计算出标     

准曲线的直线回归方程式,将样品的OD值     

代入方程式,计算出样品浓度,再乘以稀释

倍数,即为样品的实际浓度.

试剂盒性能：

1.样品线性回归与预期浓度相关系数R值为0.95以上.

2.批内与批见应分别小于9%和11%    

保存条件及有效期：

1.试剂盒保存；2-8℃.

2．有效期：6个月

9238960  Serial Cable, F9/F25, BS            CNY                              【小鼠抗子宫内膜抗体(EMAb)ELISA试剂盒厂家】

9016903  Upgrade kit, needle, w-drive, BR8           CNY                             

9016857  XYP IS V2.3                CNY                             

9232557  Vacuum manifold, plate holder 1.02                 CNY                             

9238941  PCB, CSU-01, BS15            CNY                             

9241121  Loctite 425                CNY                             

9241120  Loctite 242                CNY                             

9240957  PCB, gripper, handler, Qsym             CNY                             

9240953  Motor, z,  handler, Qsym                 CNY                             

9240781  500 ml Reagent #6 Update, AP96            CNY                             

9240780  500 ml Reagent #6 Update, AP196          CNY                             

9240772  Assy, Sub, Dispense Head, AP96                CNY                             

9240769  Mono Touch Screen, AP96                 CNY                             

9241465  IQ/OQ Services, QIAcube         Instrument qualification support service for the QIAcube. The product contains the IQ/OQ documentation ａnd the performance of the qualification protocols (travel ａnd labor costs included). CNY                             

9243647  Acceler. Valid Supp, QuantiPlex/QP Hyres        The QIAGEN Quantiplex/Quantiplex HYres Accelerated Validation Support service includes all travel ａnd labor charges for a site visit up to 5 days by a QIAGEN Specialist.  A QIAGEN specialist will provide an introductory seminar about the Quantiplex/Quantiplex HYres quantification chemistry utilized in the process, review the Handbook ａnd User Manual, as well as hands-on user reaction setup, ａnd best practices.  The Accelerated Validation service includes guidance on the laboratory’s validation experimental design, internal validation documentation support, ａnd QIAGEN sponsored data templates.  The QIAGEN Specialist will support the customer by running samples through Quantiplex/Quantiplex HYres validation, as well as general wet lab support such as pipetting for quantification to produce the data needed for the validation study. The customer is responsible for submitting the data to QIAGEN for assisted data analysis. The customer is responsible for final documentation ａnd report using QIAGEN prepared templates provided with the final product.  Enough consumable chemistry ａnd accessories (QIAGEN or other vendors) should be purchased by the customer to cover the validation experimental design ａnd are not included in the cost of validation support.  CNY                             

9243646  Guided Valid Support, Other Quant. Sys  The QIAGEN Guided Validation Support, Other Quantification System service includes all travel ａnd labor charges for a site visit up to 2 days by a QIAGEN Specialist.  A QIAGEN specialist will provide hands-on user reaction setup, ａnd best practices.  The Guided Validation service includes experimental design consultation, internal validation documentation support, ａnd QIAGEN sponsored data templates.  The QIAGEN Specialist will also aide the customer in running some samples to initiate the validation process.  Enough consumable chemistry ａnd accessories (QIAGEN or other vendors) should be purchased by the customer to cover the validation experimental design ａnd are not included in the cost of validation support. Please indicate which protocols to validate (Plexor HY, Quantifiler Human, Quantifiler Y, Quantifiler Duo)      CNY                             

9243541  #Rapid Plate, PM Service         "Service response time (repair): not applicable

Periodic inspection/maintenance: yes

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts : not included

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage"  CNY                              【小鼠抗子宫内膜抗体(EMAb)ELISA试剂盒厂家】

9243115  Lee Sample Valve, LC                 CNY                             

9242863  QIArent&go - Rent out fee QSAS Cabinet                  CNY                             

9242771  Tool, align encoder, Qsym                  CNY                             

9242717  Validation Support, 5d, QIAcube      The QIAGEN QIAcube Accelerated Validation Support service includes all travel ａnd labor charges for a site visit up to 5 days by a QIAGEN Specialist.  A QIAGEN specialist will provide an introduction to the QIAcube system ａnd the DNA Investigator silica chemistry utilized in the process, review the Handbook ａnd User Manual, as well as hands-on user support ａnd QIAcube instrument operation. The Accelerated Validation service includes guidance on the laboratory’s validation experimental design, internal validation documentation support, ａnd QIAGEN sponsored data templates.  The QIAGEN Specialist will support the customer by running samples through the QIAGEN extraction method/system, as well as general wet lab support such as pipetting for quantification, STR ａnd CE setup, normalization, etc. to produce the data needed for the validation study.  The customer is responsible for submitting the data to QIAGEN for assisted data analysis. The customer is responsible for final documentation ａnd report using QIAGEN prepared templates provided with the final product.  Enough consumable chemistry ａnd accessories (QIAGEN or other vendors) should be purchased by the customer to cover the validation experimental design ａnd are not included by QIAGEN.  Please indicate which protocols to include in the validation (QIAamp, QIAamp DNA Investigator, MinElute PCR Cleanup, etc.). CNY                             

9242204  Adapter, 2x Rotor-Disc, Qsym          CNY                             

5000-1020R     DML 2000 Luminometer, 240V (Refurbished)          CNY                             

5000-1323        Rotary Shaker - Cord Line, 220V                CNY                             

5000-1317        Knob Speed Timer, SKR1           CNY                             

5000-1272        X Drive Track, DML2                  CNY                             

5000-1260        Stepper Model New Styl, DML2                CNY                             

5001-2240        Hybrid Capture 2 (hc2)System Core, 240V                CNY                             

5000-1506        Switch ON-OFF 240V, HTR1               CNY                             

6000-4111ENV2.2   RCS User Application Manual (English)             CNY                             

6000-3117        Plate Washer I - Maintenance Kit             CNY                             

5199-1220        hc2 High-Risk HPV DNA Test             CNY                             

QS-5139   微囊藻毒素RR        Microcystin RR   ≥ 95% by HPLC         50ug                                     【小鼠抗子宫内膜抗体(EMAb)ELISA试剂盒厂家】

QS-5140   微囊藻毒素RR        Microcystin RR   ≥ 95% by HPLC         100ug                                  

QS-5141   微囊藻毒素RR        Microcystin RR   ≥ 95% by HPLC         250ug                                  

QS-5142   微囊藻毒素RR        Microcystin RR   ≥ 95% by HPLC         500ug                                  

QS-5143   微囊藻毒素RR        Microcystin RR   ≥ 95% by HPLC         1mg                            

QS-5144   黑海绵酸,软海绵酸        Okadaic acid(OA)   ≥ 95% by HPLC     25ug                                    

QS-5145   黑海绵酸,软海绵酸        Okadaic acid(OA)   ≥ 95% by HPLC     50ug                                    

QS-5146   黑海绵酸,软海绵酸        Okadaic acid(OA)   ≥ 95% by HPLC     100ug                                  

QS-5147   黑海绵酸,软海绵酸        Okadaic acid(OA)   ≥ 95% by HPLC     500ug                                  

QS-5148   黑海绵酸,软海绵酸        Okadaic acid(OA)   ≥ 95% by HPLC     1mg                            

QS-5149   鳍藻毒素         Dinophysistoxins(DTX-1)    ≥ 95% by HPLC         1mg                            

QS-5150   裸藻毒     Brevetoxin B (PbTx-2)   ≥ 98% by HPLC      1mg                            

QS-5151   裸藻毒     Brevetoxin B (PbTx-2)   ≥ 98% by HPLC      5mg                            

QS-5152   石房蛤毒素     Saxitoxin acetate     1mg                            

QS-5154   黄曲霉毒素B1        Aflatoxin B1                                          

QS-5155   黄曲霉毒素B2        Aflatoxin B2                                          

QS-5156   黄曲霉毒素G1        Aflatoxin G1                                          【小鼠抗子宫内膜抗体(EMAb)ELISA试剂盒厂家】

QS-5157   黄曲霉毒素G2        Aflatoxin G2                                         

QS-5158   黄曲霉毒素M1       Aflatoxin M1    2320                                    

QS-5159   黄曲霉毒素M2       Aflatoxin M2    6200                                    

QS-5160   桔青霉素/桔霉素   Citrinin                                          

QS-5161   呕吐毒素/        Deoxynivalenol                                             

QS-5162   脱氧雪腐镰刀菌烯醇DON    Vomintoxin                                           

QS-5163   伏马毒素B1   Fumonisin B1                                        

QS-5164   伏马毒素B2   Fumonisin B2                                        

QS-5165   赭曲霉毒素A Ochratoxin A                                        

QS-5166   棒曲霉素/展青霉素        Patulin                                          

QS-5167   T-2 毒素 T2 Toxin                                        

QS-5168   玉米赤霉烯酮/F2毒素ZEN   Zearale                                         

QS-5169   杂色曲霉素/柄曲霉素ST       Sterigmatocystin