1. 酶标仪(450nm 波长滤光片)

2. 高精度移液器，EP 管及一次性吸头

3. 37℃恒温箱，双蒸水或去离子水

4. 吸水纸

【小鼠P物质(SP)ELISA试剂盒价格】齐一生物【友情提示】：本产品仅供科研研究使用，不得用于人体临床直接检测。避免给您带来不必要的损失，请仔细阅读购买说明！

ELISA应用：

ELISA检测试剂盒、ELISAKit、酶联免疫试剂盒、酶联免疫吸附测定试剂盒、酶联免疫分析试剂盒、酶免试剂盒等,比较常见的叫法是ELISA检测试剂盒、酶联免疫吸附测定试剂盒等ELISA试剂盒自从60-70年代问世以来,得到全世界科研工作者的认可及推崇,在欧美及中国获得很大的推广,尤其是国内生化领域的长足发展.Elisa生物试验是一种敏感性高,特异性强,重复性好的实验诊断方法.由于其试剂稳定、易保存,操作简便,结果判断较客观等因素,已广泛应用在免疫学检验的各领域中.ELISA检测试剂盒适用于体外定性检测人血清或血浆中的抗人类戊型肝炎（HEV）病毒IgM抗体ELISA检测

【小鼠P物质(SP)ELISA试剂盒价格】齐一生物科技欢迎您订购咨询，齐一生物销售：021－6034 8496；181214 53965；173021 04490

齐一生物供应的ELISA试剂盒安全性提示：

1.工作人员实验中避免直接接触终止液和底物A、B。一旦接触到这些液体，请尽快用水冲洗。

2.实验中不要吃喝、抽烟或使用化妆品。

3.不要用嘴吸取试剂盒里的任何成份。

样本保存条件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6个月

3.-50℃以下的冰箱，可以放一年

【小鼠P物质(SP)ELISA试剂盒价格】齐一生物供应的ELISA试剂盒ELISA实验原理

试剂盒是固相夹心法酶联免疫吸附实验（ELISA）.已知浓度的标准品、未知浓度的样品加入微孔酶标板内进行检测.先将生物素标记的抗体同时温育.洗涤后,加入亲和素标记过的HRP.再经过温育和洗涤,去除未结合的酶结合物,然后加入底物A、B,和酶结合物同时作用.产生颜色.颜色的深浅和样品中的浓度呈比例关系.

免疫测定技术的基础在于抗原抗体之间的特异结合反应,所以任何优质的诊断试剂离不开优质的原料,如抗原抗体,酶等.以前用于免疫测定的抗原通常为各种纯化抗原,而抗体则为纯化抗原免疫动物后获得的多克隆抗体和使用杂交瘤技术得到的单克隆抗体,而抗原或抗体的标记物如酶标结合物则通过各种化学合成方法制备.近年来,随着分子生物学的发展,使用基因工程方法制备各种特殊的抗原或抗体及其酶结合物等免疫测定试剂几成为现实的新一代试剂,各种新型使用的测定方法也不断出现.

HBsAg试剂特点（检测模式：临床抗体夹心法）：包被抗体为山羊多抗.多抗具有高亲和性和对抗原各种表位的反应性.酶表抗体为与HBsAg有不同结合位点的鼠复合单位,可测得HBsAg变异样品,提高检测的特异性（99.98%）提高检测的灵敏度,应用ParlEhrich学说（PEI）HBsAg标准品,对ad标准品的灵敏度为0.05ng/ml对ay标准品灵敏度为0.025ng/ml

ELISA检测试剂盒应用定性夹心免疫检测技术,用合成的HEV多肽抗原包被微孔板板条,这些多肽是中国型HEV毒株核心氨基酸序列中抗原性很强的肽段,分别来自于该毒株的开放阅读框2和开放阅读框3.将样品或标准品加入孔中并孵育,如果其中存在HEVIgM抗体,这些抗体就会与HEV的多肽抗原结合,并固定在上面,洗板除去其它非特异性抗体和样品中的其它成份.然后加入羊抗人IgM-HRP（辣根过氧化物酶）酶结合物,经第二次孵育后,酶结合物就会与次孵育结合上的HEVIgM抗体相结合,洗板除去未结合的酶结合物,加入TMB底物溶液,在第三次孵育时会发生酶-底物反应,只有那些含有HEVIgM抗体和酶结合物所形成的复合物的孔才会发生颜色变化,加入硫酸溶液终止酶和底物间的反应,并在波长:450nm处测量O.D.值,按照本HEVIgM抗体elisa试剂盒的测试标准,O.D.值大于或等于Cut-Off值的样品被认为是初试阳性. 【小鼠P物质(SP)ELISA试剂盒价格】

以上资料仅供参考了解，不供于论文写作

齐一生物专业为您提供高品质，高灵敏度.高质量的酶联免疫试剂盒，齐一生物长期经营ELISA试剂盒（免费代测）及抗体、分子学生物试剂盒、金标法检测试剂盒、比色法检测试剂盒、细胞菌株、培养基、细胞因子、生化试剂、耗材等生物试剂产品。齐一生物销售：021－6034 8496；181214 53965；173021 04490

9010830  Einschubplatte 4DeepWellRackRose-80 1177          CNY                              【小鼠P物质(SP)ELISA试剂盒价格】

981601     Rotor-Disc Heat Sealing Film (60)             CNY                             

9012923  Motor, Y, SamTrack, P3xxx                CNY                             

931236     QIAsymphony DNA Mini Kit (192)             CNY                             

133256     NeXtalStock HEPES sod. salt pH 7.4 (200)                 CNY                             

133253     NeXtalStock Sodium phosphate pH6.2 (200)            CNY                             

133247     NeXtalStock Sodium succinate pH5.5 (200)              CNY                             

929609     QX Pro Labeling Buffer (25 ml)                   CNY                             

330501     RT² SYBR Green qPCR Mastermix (8)                CNY                             

330451     RT² PreAMP cDNA Synthesis Kit (12)                 CNY                             

330431     RT² Microfluidics qPCR Reagent System           CNY                             

210210     QIAGEN OneStep RT-PCR Kit (25)    For 25 reactions: QIAGEN OneStep RT-PCR Enzyme Mix, 5x QIAGEN OneStep RT-PCR Buffer,* dNTP Mix,** 5x Q-Solution, RNase-free water CNY                             

9012758  Magnetic Stirrer, single position               CNY                             

9010345  Cable, X, flat, 1400 mm,  (Size120)                 CNY                             

9010192  X-Top cover,  P3xx0 (9194)              CNY                             

979103     PyroMark Q96 HS Nucleotide Tip (8)       Re-usable tips for dispensing nucleotides (NDTs) for use in PyroMark Q96 MD. Each package contains 8 tips.  CNY                             

301806     HiPerFect HTS Reagent (2 x 1 ml)             CNY                              【小鼠P物质(SP)ELISA试剂盒价格】

9002241  QIAsymphony SP/AS, PrioPLUS         "QIAsymphony SP/AS Instrument

Installation of QIAsymphony SP/AS  instrument & Introductory training

Warranty extension to 3 yrs

On site service, 48 hr response

Preventive Maintenance (1 onsite PM per year)

Priority service provision over non-contract customers

Documented installation & PM report

Unlimited number of repair service

All cost covered (travel, labor, parts)"     CNY                             

9012713  Z-motor (Plato 7) right              CNY                             

9012695  G-motor Plato 7                 CNY                             

57704       QIAamp MinElute Virus Spin Kit (50)       For 50 minipreps: 50 QIAamp MinElute Columns, QIAGEN Protease, Carrier RNA, Buffers, Collection Tubes (2 ml)        CNY                             

929554     QX DNA Size Marker 50 bp-1,5 kb (100 µL)               CNY                             

764133     PAXgene Bone Marrow RNA Kit (30)       "For storage ａnd transport of bone marrow samples ａnd stabilization ａnd purification of intracellular RNA

Integrated system for storage, stabilization, ａnd purification

Immediate stabilization of intracellular RNA at collection

Rapid purification from heterogeneous bone marrow samples

Safe ａnd easy transportation"          CNY                             

762174     PAXgene Blood RNA Kit (50), CE                CNY                             

203646     HotStarTaq Plus Master Mix Kit (2500)   HotStarTaq Plus Master Mix Kits offer all the benefits of great specificity, sensitivity, ａnd performance as HotStarTaq Plus DNA Polymerase NOW in a ｍaster mix format, which offers even faster results ａnd added convenience.   CNY                             

203605     HotStarTaq Plus DNA Polymerase (1000)         4 x 250 units HotStarTaq Plus DNA Polymerase, 10x PCR Buffer, 10x CoralLoad PCR Buffer, 5x Q-Solution, 25 mM MgCl2         CNY                             

201443     Taq PCR Master Mix Kit (250) 3 x 1.7 ml Taq PCR Master Mix‡ containing 250 units Taq DNA Polymerase total, 3 x 1.7 ml distilled water     CNY                             

180964     GeneRead Gene Panel Mastermix (4.8 ml)               CNY                             

180485     GeneRead DNA Library L Amp Kit (100)           CNY                             

180455     GeneRead DNA Library I Amp Kit (100)            CNY                             

159205     QIAcard FTA Four Spots (100)           CNY                             

159203     QIAcard FTA Two Spots (100)            CNY                             

QY-19322 Anti-Visfatin-1/PBEF1(NT)内脂素/内脏脂肪素/腹脂素/内肥素（小鼠抗人、大、小鼠、猴：N端多肽）                                                  

QY-19332 Anti-Visfatin-1/PBEF1(NT) hu、 mo、 rat、MK内脂素/内脏脂肪素(N端抗体)                                                      

QY-19342 Anti-Visfatin-1/PBEF1(NT) Human内脂素/内脏脂肪素（N端抗体）                                               

QY-19352 Anti-Visfatin-2(CT)hu、mo、rat、 MK内脂素/内脏脂肪素（C端抗体）                                                       

QY-19362 Anti-VSIG4 (V-set ａnd immunoglobulin domain-containing protein 4)AntibodyT淋巴细胞负调节蛋白抗体                                                     

QY-19372 Anti-VTG(vitellogenin)卵黄蛋白原抗体                                              

QY-19382 Anti-VWF (Von Willebrand Factor)血管假性血友病因子/血管性血友病因子抗体                                                   

QY-19392 Anti-WNK4(Ser/Thr-protein kinase WNK4; protein kinase with no lysine 4; protein kinase, lysine –dficient 4)一种新的丝氨酸/苏氨酸激酶家族成员的基因WNK4抗体                                                      

QY-19402 Anti-WWOX （WW domain-containing oxidoreductase）包含氧化还原酶的WW域抗体                                                      

QY-19412 Mouse Anti-YERSINIA PESTIS Monoclonal Antibody鼠抗鼠疫F1蛋白/鼠疫耶尔森氏菌荚膜抗原F1单克隆抗体                                                

QY-19422 anti-XIAP（X-linked Inhibitor of Apoptosis Protein）X-连锁凋亡蛋白/性连锁凋亡抑制蛋白抗体                                                    【小鼠P物质(SP)ELISA试剂盒价格】

QY-19432 anti-XAF1（XIAP-associated factor 1）XIAP相关因子1抗体                                                    

QY-19442 Anti-XRCC1（X-ray Repair Cross Complementing 1）X射线修复交叉互补蛋白/细胞碱基切除修复基因抗体                                                       

QY-19452 Anti-ZCWCC1(Zinc finger CW-type coiled-coil domain protein 1)ZCWCC1抗体                                                    

QY-19462 Anti-Z-DEVD-FMK(CASPASE3/CPP32 INHIBITOR W-3)CASPASE3凋亡抑制剂                                                       

QY-19472 Anti-ZNF268 (zinc finger protein ZNF268)- middle (1b)锌指脂蛋白-1b抗体                                              

QY-19482 Anti-ZNF268 (zinc finger protein ZNF268)- middle (1c)锌指脂蛋白-1c抗体                                               

QY-19492 Anti-ZNF268 (zinc finger protein ZNF268)- N-terminal (1a) Anti-ZNF268 (zinc finger protein ZNF268)- N-terminal (1a)锌指脂蛋白-1a抗体                                                

QY-19502 Anti-ZNF300 (zinc finger protein ZNF300)- middle锌指蛋白300抗体                                               

QY-19512 anti-ZO-1（zonula occludens-1）胞质紧密粘连蛋白1抗体                                                      

QY-19522 Anti-β-Actin/HRP辣根过氧化物酶标记β-肌动蛋白（抗体）                                              

QY-19532 Anti-人T亚群/NK细胞检测试剂盒人T淋巴细胞亚群检测盒 （T-SuRCet）                                                

QY-19542 CD2AP (CD2-associated protein)白细胞分化抗原CD2AP                                                    

 QY-02000         新的抑癌基因Brg1抗体      Anti-Brg1（brahma-related gene 1）                                       

 QY-02001          抗布鲁氏菌抗体     Anti-Brucella                                       

 QY-02002          乳腺癌转移抑制基因1抗体          Anti-BRMS-1 (Breast cancer metastasis-suppressor 1)                                       

 QY-02003          兔抗牛血清白蛋白抗体         Anti-BSA                                       【小鼠P物质(SP)ELISA试剂盒价格】

 QY-02004          胆汁酸盐输出泵蛋白抗体     Anti-BSEP(Bile salt export pump)                                        

 QY-02005          B细胞迁移基因2抗体           Anti-BTG2/TIS21(B-cell translocation gene 2)                                              

 QY-02006          过敏毒素C5a（补体C5a）抗体   Anti-C5a