价格详细资料：试剂盒价格电议，齐一生物销售：021－6034 8496；181214 53965；173021 04490,齐一生物科技（上海）有限公司提供ELISA试剂盒受到了广大研究所.中科院科研单位一致肯定和认同。齐一生物大品牌保证，倾力为国内外科研院校实验室提供*优质产品。

【小鼠血管舒缓激肽(BK)ELISA试剂盒厂家】注意事项

1. 当混合蛋白溶液时应尽量轻缓，避免起泡。

2. 洗涤过程非常重要，不充分的洗涤易造成假阳性。

3. 一次加样时间控制在5分钟内，如标本数量多，推荐使用排枪加样。

4. 请每次测定的同时做标准曲线，做复孔。

5. 如标本中待测物质含量过高，请先稀释后再测定，计算时请*后乘以稀释倍数。

6. 在配制标准品、检测溶液工作液时，请以相应的稀释液配制，不能混淆。

7. 底物请避光保存。

8. 不要用其它生产厂家的试剂替换试剂盒中的试剂。

FOR RESEARCH USE ONLY

Drug Names

Generic Name：

This kit can be used for determination of serum, plasma ａnd liquid samples Organization Content.

The experimental principle:

The product levels were measured in samples of the kit by double antibody sandwichmethod. The product with the purified antibody coated microtiter plate, made of solid phase antibody, to package is the product antigen monoclonal antibodies are then added to the micropores, the product and then with HRP labeled antibody binding, the formation of antibody - antigen - antibody complex enzyme label, after thorough washing with TMB chromogenic substrate. TMB in the HRP enzyme catalytic conversion into the blue, and in the action of acid into the final yellow. This product is positively related to the depth of color and in the samples. Instrument measured absorbance in the 450nm wavelength with ELISA (OD), the product concentration in the samples was calculated by standard curve.

Materials provided with the kit

Materials provided with the kit 96 determinations Storage

User manual   1  

Closure plate membrane   2  

Sealed bags   1  

Microelisa stripplate    1   2-8℃

Standard：360ng/L 0.5ml×1 bottle   2-8℃

Standard diluent  1.5ml×1 bottle   2-8℃

HRP-Conjugate reagent    6ml×1 bottle 2-8℃

Sample diluent    6ml×1 bottle 2-8℃

Chromogen Solution A 6ml×1 bottle 2-8℃

Chromogen Solution B 6ml×1 bottle 2-8℃

Stop Solution 6ml×1 bottle 2-8℃

wash  solution    （20ml×30 fold）

×1bottle  2-8℃

Specimen requirements

1.    serum- coagulation at room temperature 10-20 mins，centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. 【小鼠血管舒缓激肽(BK)ELISA试剂盒厂家】

2.    plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

3.    Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax ａnd cerebrospinal fluid Reference to it.

4.    cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS（PH7.2-7.4）, Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells ａnd release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

5.    Tissue samples- After cutting samples, check the weight,add PBS（PH7.2-7.4）, Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS（PH7.4）, Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant.

6.    extract as soon as possible after Specimen collection,and according to the relevant literature, ａnd should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.

7.    Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.

Assay procedure

1.Dilute ａnd add sample to Standard: set 10 Standard wells on the ELISA plates coated, add Standard 100μl to the first ａnd the second well, then add Standard dilution 50μl to the first ａnd the second well, mix; take out 100μl form the first ａnd the second well then add it to the third ａnd the forth well separately. then add Standard dilution 50μl to the third ａnd the forth well ,mix ; then take out 50μl from the third ａnd the forth well discard, add 50μl to the fifth ａnd the sixth well ,then add Standard dilution 50μl to the fifth ａnd the sixth well, mix ; take out 50μl from the fifth ａnd the sixth well ａnd add to the seventh ａnd the eighth well, then add Standard dilution 50μl to the seventh ａnd the eighth well ,mix ; take out 50μl from the seventh ａnd the eighth well ａnd add to the ninth ａnd the tenth well, add Standard dilution 50μl to the ninth ａnd the tenth well, mix , take out 50μl from the ninth ａnd the tenth well discard(add Sample 50μl to each well after Diluting ,(density: 240ng/L,160ng/L ,80ng/L,40ng/L, 20ng/L)

2.add sample：Set blank wells separately (blank comparison wells .don’t add sample ａnd HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, ａnd Gently mix. 【小鼠血管舒缓激肽(BK)ELISA试剂盒厂家】

3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.

4.Configurate liquid: 30-fold (or 20-fold)wash solution diluted 30-fold (or 20-fold) with distilled water ａnd reserve.

5.washing：Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.

6.add enzyme：Add HRP-Conjugate reagent 50μl to each well, except  blank well.

7.incubate：Operation with 3.

8.washing：Operation with 5.

9.color：Add Chromogen Solution A 50ul ａnd Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃

10.Stop the reaction：Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).

11.assay：take blank well as zero , Read absorbance at 450nm after Adding Stop Solution ａnd within 15min.

Important notes

1.    The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.

2.    washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.

3.    add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 mins, if the number of sample is much , recommend to use Volley . 【小鼠血管舒缓激肽(BK)ELISA试剂盒厂家】

4.    if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente ａnd multiplied by the dilution factor.（×n×5）.

5.    Closure plate membrane only limits the disposable use, to avoid cross-contamination.

6.    The substrate evade the light preservation.

7.    Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.

8.    All samples, washing buffer ａnd each kind of reject should according to infective material process.

9.    Do not mix reagents with those from other lots.

Calculate：

Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density ａnd the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.

Storage ａnd validity

1．Storage：  2-8℃.

2．validity： six months.

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334471     EpiTect ChIP OneDay Kit (12)            CNY                             

331260     miScript PreAMP Custom Primer Mix                CNY                             

331001     RT² miRNA qPCR Assay (200)            CNY                             

9010969  Plate stacker, detachable         Unit for stacking up to ten 96-well plates; for use with the robotic handling system      CNY                             

9010928  Motor, X, SamTrack, P3xxx                CNY                             

979302     PyroMark Q24 Cartridge (3) CE        For delivery of nucleotides ａnd reagents for use in PyroMark Q24. CE-Marked      CNY                             

981303     Rotor-Disc 72 (240)           CNY                             

981005     PCR Tubes, 0.2 ml  (1000)                 CNY                             

9012976  Gripper, left, landoplasted, MTP 120                CNY                             

931955     QIAsymphony Certal Vaccine NA Kit (96)                   CNY                             

929707     QX Cartridge Stand Cover (1)            CNY                             

133252     NeXtalStock Sodium phosphate pH 7 (200)               CNY                             

929606     QX FA Separation Buffer (40 ml)                CNY                             

9010509  Motor, turntable 1/2 MTP, P3/7                  CNY                             

69525       mericon DNA Bacteria Kit (100)                CNY                              【小鼠血管舒缓激肽(BK)ELISA试剂盒厂家】

69106       DNeasy Plant Mini Kit (250)     250 DNeasy Mini Spin Columns, 250 QIAshredder Mini Spin Columns, RNase A, Buffers, Collection Tubes (2 ml)     CNY                             

211015     QuantiTect Virus Kit (1000)               CNY                             

206674     Type-it CNV SYBR Green PCR +qC Kit (400)               CNY                             

9012763  Liquid Handling Kit Plato 1300                   CNY                             

59568       EpiTect Control DNA (1000)              CNY                             

979014     MOTT 16S Primers For PCR               CNY                             

979011     PyroMark Q96 Vacuum Prep Trough (5)  Re-usable plastic troughs for use in all PyroMark Q96 Vacuum Workstations.         CNY                             

59932       EpiTect MethyLight Assay Hs-ONECUT2(100) "10-fold primer TaqMan probe mix, for real-time MethyLight PCR

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59695       EpiTect PCR Control DNA Set (100)           CNY                             

206343     Type-it Mutation Detect PCR Kit (200)    "The Type-it Mutation Detect PCR Kit is especially developed for to fast ａnd reliable detection of mutations such as deletions, insertions,and translocations. It is also highly suited for multiplex PCR-based preamplification of SNPs as preparation for genotyping systems like the

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Features

 Successful & reproducible analysis of multiple mutations

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Features

 Successful & reproducible analysis of multiple mutations

 Optimization-free multiplex PCR assay development

 Specific ａnd sensitive coamplification of all fragments

 Optimized protocol for fast ａnd reliable results"        CNY                             

9012719  Set, Pins, (6 small, 4 large)                CNY                             

929561     QX Size Marker 50 - 800 bp v2.0 (50 µl)            CNY                             

972804     PyroMark Gold Q96 Reagents (5 x 96)     "For performing pyrosequencing reaction on the ID (5x 96) ａnd Q96 MD (15x96)

ID needs more reagents"         CNY                             

301705     HiPerFect Transfection Reagent (1 ml)             CNY                             

 QY-02067          细胞表面趋化因子受体2A抗体  Anti-CCR-2A(C-C Chemokine receptor 2A)                                            【小鼠血管舒缓激肽(BK)ELISA试剂盒厂家】

 QY-02068          细胞表面趋化因子受体2B抗体  Anti-CCR-2B(C-C Chemokine receptor 2B)                                           

 QY-02069          细胞表面趋化因子受体3抗体     Anti-CCR-3(CC chemokine receptor 3)                                         

 QY-02070          细胞表面趋化因子受体4抗体     Anti-CCR-4(C-C chemokine receptor 4)                                       

 QY-02071          细胞表面趋化因子受体6抗体     Anti-CCR-6/CD196(C-C chemokine receptor type 6)                                             

 QY-02072          细胞表面趋化因子受体6抗体     Anti-CXC-R6(CXC-chemokine receptor 6)                                             

 QY-02073          细胞表面趋化因子受体7抗体     Anti-CCR-7(C-C chemokine receptor 7)                                       

 QY-02074          CXCL10趋化因子10/干扰素诱导蛋白10抗体    anti-CXCL10/IP-10                                       

 QY-02075          细胞表面趋化因子受体8抗体     Anti-CCR-8(C-C chemokine receptor 8)                                       

 QY-02076          细胞表面趋化因子受体9抗体     Anti-CCR-9(C-C chemokine receptor 9)                                       

 QY-02077          粘膜相关上皮趋化因子MEC抗体        Anti-CCL28(CC Chemokine Ligand 28)                                          

 QY-02078          细胞表面趋化因子受体10抗体   Anti-CCR-10(C-C chemokine receptor 10)                                            

 QY-02079          细胞周期相关激酶抗体         Anti-CCRK(Cell cycle related kinase)                                            

 QY-02080          CD10抗体        Anti-CD10/CALLA (Neutral Endopeptidase)                                        

 QY-02081          CD105/转化生长因子β受体抗体          Anti-CD105/Endoglin/TGF-β receptor                                      

 QY-02082          干细胞生长因子受体/细胞表面分化抗原抗体   Anti-CD117/c-kit/SCFR                                         

 QY-02083          抗磷酸化原癌基因c-kit抗体         Anti-phospho-c-kit/SCFR/CD117(pTyr936)                                            【小鼠血管舒缓激肽(BK)ELISA试剂盒厂家】

 QY-02084          造血干细胞抗原CD133抗体         Anti-CD133 antigen                                             

 QY-02085          多配体蛋白聚糖1抗体          Anti-CD138/Syndecan-1                                      

 QY-02086          黑色素瘤细胞粘附分子CD146抗体    Anti-CD146/MCAM                                     

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 QY-02088          氨肽酶N抗体         Anti-CD13/ANPEN(Aminopeptidase N)                                        

 QY-02089          CD14/内毒素受体抗体   Anti-CD14(cluster of differentiation antigen 14)                                         

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 QY-02095          NK细胞抑制性受体3DL1抗体     Anti-CD158e/KIR3DL1(Killer cell immunoglobulin-like receptor 3DL1)                                       

 QY-02096          CD167a抗体   Anti-CD167a/DDR1