【友情提示】：本产品仅供科研研究使用，不得用于人体临床直接检测。避免给您带来不必要的损失，请仔细阅读购买说明！

本试剂盒仅供研究使用，齐一生物销售：021－6034 8496；181214 53965；173021 04490欢迎选购！

操作步骤

1.准备试剂，样品和标准品

2.加入准备好的样品和标准品，37℃反应30分钟

3.洗板5次，加入酶标试剂，37℃反应30分钟

4.洗板5次，加入显色液A、B，37℃显色15分钟

5.加入终止液

6.15分钟之内读OD值

7.计算
齐一生物供应ELISA试剂盒检测的目的是为实验提供准确可靠的定量分析实验依据。为了保证实验数据的可靠性，在实验过程中必须坚持全面质量控制和全过程质量控制。在收集标本前都必须有一个完整的计划。

【小鼠细胞周期素D3(Cyclin-D3)ELISA试剂盒价格】样本保存条件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6个月

3.-50℃以下的冰箱，可以放一年

【小鼠细胞周期素D3(Cyclin-D3)ELISA试剂盒价格】样本收集

1.血清样本用干燥管（红色）或者促凝管（黄色）收集

2.血浆用抗凝管，一般有EDTA抗凝（紫色）、枸橼酸钠抗凝（黑色或者蓝色）

3.动物组织匀浆（细胞）：

              1g组织加入9ml PBS（浓度为0.1M，pH为7.4的）测分泌型指标

              1g组织加入1ml组织裂解液加入8ml PBS 测细胞内

粪便样本：1g样本+9ml PBS（浓度为0.01M，pH为7.4的）

没有PBS的话也可以用一下生理盐水，还是用PBS

【小鼠细胞周期素D3(Cyclin-D3)ELISA试剂盒价格】样本离心转速及时间

1.试管采集的样本：3000转，离心五分钟取上清

2.离心管或者EP管采集的样本：5000－－8000转3分钟取上清

3..样本保存－－如果样品不立即使用，应将其分成小部分-70 ℃保存，避免反复冷冻。尽可能的不要使用溶血或高血脂血。如果血清中大量颗粒，检测前先离心或过滤。不要在37℃或更高的温度加热解冻。应在室温下解冻并确保样品均匀地充分解冻。

【小鼠细胞周期素D3(Cyclin-D3)ELISA试剂盒价格】以上样本采集及处理方式供您参考，如有问题请来电，齐一生物销售：021－6034 8496；181214 53965；173021 04490

9238685  Mains switch ETA, 10 A              CNY                            【小鼠细胞周期素D3(Cyclin-D3)ELISA试剂盒价格】

9231656  Holder, CCD Camera BL-185        CNY                           

9231538  Needle, W-Drive, DISU, BR8        CNY                           

9014876  Microplate Station Adapter            CNY                           

9014556  Shaker Adapter 8000  24-tubes  13ml              CNY                           

9014499  Disposable Tip Station, BR8, RD v1.0         CNY                           

9022574  Strut assembly, Hood, SP, right, Qsym        CNY                           

9238348  Upgrade, Precision Pippeting Precision pipetting upgrade includes onsite installation ａnd calibration visit including all travel ａnd labor to install the precision pipetting system, as well as the following components: 4x100ul syringes, 4x 3 Port Valves, 24x96 10ul Disposable Tips ａnd a precision Pipetting Calibration Kit.    CNY                           

9022379  Notebook, RGQ            CNY                           

9021984  Transport lock, QXA            CNY                           

9021892  Bracket, optical sensor, angled, QIAC          CNY                           

9021613  Kit, upgrade QSSP Cabinet, Qsym        CNY                           

9238605  Centrifuge Motor, complete          CNY                           

9238552  RS232 box, online fill ready, RapidPlate              CNY                           

9238253  Syringe Seal           CNY                           

9238222  De-Bubbler Top             CNY                           

9240674  Clamp Kit, Sensor, Reagent Jar, AP96/196           CNY                            【小鼠细胞周期素D3(Cyclin-D3)ELISA试剂盒价格】

9240665  Nut, Flangeless,1/4-28, White, AP        CNY                           

9240656  Rebuild Kit, Gast Pump, AP96             CNY                           

9240646  External BCR, 2D, HHP, AP          CNY                           

9240619  Fuse, TE5, 2.0A, PCB, AP96/196         CNY                           

9240610  Hall Effects Sensor, Latch Lock, AP98        CNY                           

9240609  Fiber Optic Sensor, Sub-unit, AP98             CNY                           

9240587  Vacuum Switch, Pump, AP96              CNY                           

9240579  Vacuum Switch Assy, Shaker, AP96            CNY                           

9240577  Cable Assy, Capper, X52 CW, AP96/196             CNY                           

9240525  Cable, Motor Axis #2, Elevator, AP98          CNY                           

9240518  Communication Module, RS232-485, AP196              CNY                           

9240502  PM Kit, AP96         CNY                           

9240348  Autopure, Premium Agreement     The Premium Cover Agreement Autopure provides full service coverage for the Autopure LS System. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 24 hours (1 working day).  CNY                           

9240241  Housing, disposable tip adapter, TR             CNY                           

9239957  EZ1, PM Service    Service covers all travel ａnd labor costs plus all necessary parts to perform a Preventative Maintenance. The instrument will also be thoroughly checked ａnd inspected to determine if any additional parts are required to get the instrument running to its optimal performance. There will be an additional charge for any such parts if any are needed.   CNY                           

QY-02050       GHRF (GHRH)生长激素释放激素（因子）（抗原）         0.5mg/6501mg/1100                         

QY-08000       ANGPTL1/ANG-1 (Angiopoietin-like Protein 1)血管位蛋白样1/血管生成素样蛋白-1抗原           0.5mg/6501mg/1100                         

QY-03840       GLUT4(Glucose transporter type 4）葡萄糖转运蛋白4（抗原）            0.5mg/6501mg/1100                          【小鼠细胞周期素D3(Cyclin-D3)ELISA试剂盒价格】

QY-00980       GIP （Gastric Inhibitory polypeptide）胃泌素抑制肽（抗原）               0.5mg/6501mg/1100                         

QY-02080       GLP-2（Glucagon-like peptide-2）胰高血糖素样肽-2（多肽抗原）               0.5mg/6501mg/1200                         

QY-01940       GRP94 （gp96）94kDa 糖调节蛋白（抗原）          0.5mg/6501mg/1100                         

QY-03480       G Protein(Guanine nucleotide-binding regulatory protein)G蛋白（鸟苷酸结合蛋白）（抗原）              0.5mg/6501mg/1100                         

QY-02520       GR (Glucocorticoid receptor)糖皮质激素受体 多肽          0.5mg/6501mg/1100                         

QY-00230       GSK-3β(NT) (Glycogen Synthase Kinase-3β)糖原合酶激酶-3β（抗原）           0.5mg/6501mg/1100                         

QY-03410       CKR-L2（ G protein-coupled receptor-2 ）G蛋白偶联受体-2(抗原)             0.5mg/6501mg/1100                         

QY-00280       GSK-3β, (Glycogen Synthase Kinase-3)葡萄糖合成激酶-3（抗原）             0.5mg/6501mg/1100                         

QY-01360       GTP-CH-1三磷酸鸟苷环水解酶（抗原）         0.5mg/6501mg/1100                         

QY-03440       H5N1-M2（Avian influenza Matrix Protein-2）H5N1流感病毒M2型（抗原）              0.5mg/6501mg/1100                         

QY-02210       HCV-Core丙型肝炎病毒-C区（多肽）             1mg/7002mg/1300                           

QY-02180       HCV-NS1丙型肝炎病毒-NS1（抗原）              0.5mg/6501mg/1100                         

QY-02190       HCV-NS3丙型肝炎病毒-NS3（抗原）              0.5mg/10001mg/1800                       

QY-02130       HCV-NS4a丙型肝炎病毒-NS4a（抗原）           0.5mg/10001mg/1800                       

QY-02120       HEV戊型肝炎病毒（抗原）               0.5mg/6501mg/1100                         

QY-03380       HGV（Hepatitis G vivus）庚型肝炎病毒（抗原）            0.5mg/6501mg/1100                          【小鼠细胞周期素D3(Cyclin-D3)ELISA试剂盒价格】

QY-02870       HisX6聚组氨酸标签蛋白             0.5mg/8001mg/1400                         

QY-02390       HIV-1(gp41)艾滋病病毒（抗原）               0.5mg/6501mg/1100                         

QY-02410       HIV gp120艾滋病病毒（抗原）         0.5mg/6501mg/1100                         

QY-01910       HSP-60（ Heat shock protein-60）热休克蛋白-60（抗原）            0.5mg/6501mg/1100                         

QY-01260       HSP-70 (Heat shock protein-70)热休克蛋白-70（多肽抗原）          0.5mg/6501mg/1100                         

QY-02140       ICAD（Inhibitor of Caspase-Activated DNase ）Caspase 激活的脱氧核糖核酸酶抑制剂（抗原）            0.5mg/6501mg/1100                         

QY-00180       IDE, (Insulin degradation enzyme)胰岛素降解酶（抗原）               0.5mg/6501mg/1100                         

QY-02270       IGF-1 R 人胰岛素样生长因子-I受体         0.5mg/6501mg/1100                         

QY-04060       IGFBP-5 ( Insulin-like Growth Factor Binding Protein 5、IBP5)胰岛素样生长因子结合蛋白-5(抗原)          0.5mg/6501mg/1100                         

QY-00560       Isulin胰岛素（抗原）          0.5mg/6501mg/1100                         

QY-08490       apoA1 (Apolipoprotein/APOA1 protein )human载脂蛋白A1抗原             0.5mg/6501mg/1100