齐一生物销售：021－6034 8496；181214 53965；173021 04490

1。我必须运行所有的标准和样品的重复？

是的，重复的运行，以监测测定的精度和提高检测结果的信心。

2。我必须在一次运行所有的样品吗？

不，每个试剂盒采用stripwell微孔板。这允许用户在不同的时间来分析不同数量的样品。

3。什么类型的可重复性的结果，得到与分析？

每一个工具都有一个包含一个典型数据的图表的手册。在运营商的任何变化，移液和洗涤技术，培养时间、温度、试剂盒的年龄可以引起变化的结果。每个用户都应该得到自己的标准曲线。

4。是否有可能储存的试剂比表示？

在不推荐使用条件下的部件组件的存储，以保证测试的正确性能。

5。我应该如何储存我的样品？

样品应保存在-20°C或更低的温度。长期储存，建议冻结他们在- 70余。

6。我可以修改协议吗？

BG的ELISA试剂盒进行了优化，提供*佳的结果。修改的格式或协议可能会给出错误的结果。

7。我可以使用一个不建议在试剂盒插入的样本类型吗？

该试剂盒已被验证在试剂盒插入的样品类型。未经检验的样品类型。为进一步信息的联系技术服务。

8。我的样本产生的值，在动态范围内的测定。我可以使用这些值吗？

建议只有在标准曲线的范围内下降的样本值。标准曲线的范围内的值一般是非线性的，这可能导致不正确的外推值。产生值高于标准的样品应为（进一步）稀释和重复。如果样品低于该测定的范围内，该样品被认为是不可检测的。

9。我每次都要运行一个空白或零标准吗？

是的，这些都是必需的计算，并反映任何微妙但显着的性能变化，从一天到一天，检测到检测。当排除特定的检测问题的来源时，它们也非常有帮助。

10。我能改变我在检测中使用的样本量吗？

不建议你改变卷由于BG工具被设计为*佳的性能在给定的体积

11。可以使用不同的组件组件吗？

每一个组件包含有特定批号的组件，以确保所有的组件都进行优化，以及与组件中的所有其他组件。对这些特定的地段进行质量检验。这是从来没有建议使用你自己的组件或组件从其他包或供应商。

12。我的标准曲线看起来很好，但我没有得到一个信号，我的样本，当我预期，为什么？

样品不包含被分析物。一个矩阵的效果可能是掩盖检测。确保推荐的稀释液在试剂盒插入后按规定进行。如果建议稀释，请检查是否正确进行稀释。过稀释会导致样品在标准曲线的范围内下降。

13。你怎么推荐我洗盘子？

如果您使用的是一个自动平板洗衣机，我们建议定期检查校准，并将该系统在洗涤前冲洗缓冲液冲洗。手动洗衣机也是一样的。一个中继器或洗瓶也可以使用。用户应小心，以确保所有的内容都是送气和板拍干的无尘纸。【大鼠抗精子抗体(AsAb)ELISA试剂盒现货】

14。我需要用一个盘子吗？

可靠的结果可以得到无板振动，但外径的一般会低于那些使用平板振动器。

15。为什么我要用波长校正450-570nm之间？

对于酶联免疫吸附试验，在双波长读的是做正确的光密度贡献的塑料以及，灯和光学波动。

16。如果我提我的样本，我还需要遵循推荐的稀释液试剂盒中插入了吗？

提取过程中所需的样本稀释量将受到影响的纯化和浓度的影响的协议使用。稀释或浓缩的金额将由*终用户决定。

17。什么是预期的分析物浓度，我应该找到？

一个给定的分析物的量可能会有所不同，不仅从物种的物种，但也组织和细胞来源。这个信息的*佳来源是目前的文献，很容易通过互联网在多个科学数据库访问。

18。我的光密度有点高（或较低），比那些在手册中，与我的试剂盒来了。为什么？

光学密度受影响

1.    Do I have to run all of my standards ａnd samples in duplicate?

Yes, the duplicates are run in order to monitor assay precision ａnd increase confidence in the assay results obtained.

2.    Do I have to run all of my samples at one time?

No, each kit uses stripwell microplate. This allows the user to analyse different numbers of samples at different times.

3.    What types of reproducible results are obtained with the assays?

Each kit comes with a manual containing a graph of typical data obtained. Any variation in operator, pipetting ａnd washing technique, incubation time or temperature, ａnd kit age can cause variation in result. Each user should obtain their own standard curve.

4.    Is it possible to store the reagents other than indicated?

Storage of the kit components under conditions other than indicated is not recommended in order to assure proper performance of the test.

5.    How should I store my samples?

Samples should be stored at -20oC or lower temperature. For long-term storage, it is recommended to freeze them at -70oC -80oC.

6.    Can I modify the protocol?

BG ELISA kits have been optimized to provide the best possible results. Modifying the format or protocol may give inaccurate ａnd wrong results.

7.    Can I use a sample type that is not recommended in the kit insert?

The kit has been validated for the sample types listed in the kit insert. Sample types other than those validated have not been tested. Contact Technical Service for further information.

8.    My samples generated values that were outside the dynamic range of the assay. Can I use these values?

It is recommended that only sample values that fall within the range of the standard curve be used. Values outside the range of the standard curve are generally non-linear, which can lead to incorrectly extrapolated values. Samples that generate values higher than the highest standard should be (further) diluted ａnd the assay repeated. If samples fall below the range of the assay, the sample is considered to be non-detectable. 【大鼠抗精子抗体(AsAb)ELISA试剂盒现货】

9.    Do I have to run a Blank or Zero Standards every time?

Yes, these are required for the calculations, ａnd reflect any subtle but significant performance changes from day to day ａnd assay to assay. They are also extremely helpful when troubleshooting the source of a particular assay problem.

10.  Can I alter the volume of sample I use in the assay?

It is not recommended that you alter the volumes since all BG kits are designed for optimal performance at the given volumes

11.  Can components from different kits be used?

Each kit contains components which have specific lot numbers to ensure that all of the components are performing optimally alone, as well as with all of the other components in the kit. QC testing is performed on these specific lots. It is never recommended to use your own components or components from other kits or vendors.

12.  My standard curve looked fine, but I didn’t get a signal in my sample when I expected to, why?

The sample may not contain the analyte. A matrix effect may be masking the detection. Ensure that the recommended dilution was followed as stated in the kit insert. If dilution was recommended, check to be sure that the dilution was performed properly. Over-dilution may cause the sample to fall below the range of the standard curve.

13.  How do you recommend I wash my plate?

If you are using an automated plate washer we recommend that the calibration be checked on a regular basis, ａnd that the system is flushed with the Plate Washing Buffer prior to washing. The same is true for a manual washer. A repeater or a wash bottle can also be used. The user should be careful to ensure that all of the contents are aspirated ａnd the plate tapped dry on lint-free paper.

14.  Do I need to use a plate shaker?

Reliable results can be obtained without a plate shaker, but the O.D.＇s will generally be lower than those obtained using a plate shaker.

15.  Why do I have to use wavelength correction between 450-570nm?

For the ELISA assay, reading at dual wavelengths is done to correct for the optical density contributed by the plastic well, the lamp ａnd optical fluctuations. 【大鼠抗精子抗体(AsAb)ELISA试剂盒现货】

16.  If I extract my sample, do I still need to follow the recommended dilutions given in the kit insert?

The amount of sample dilution needed after an extraction procedure will be affected by the effects of purification ａnd concentration in the protocol used. The amount of dilution or concentration will have to be determined by the end-user.

17.  What is the expected concentration of analyte that I should expect to find?

The amount of a given analyte may vary not only from species-to-species, but also between tissue ａnd cellular sources. The best source of this information is the current literature that is easily accessed through the Internet at multiple scientific databases.

18.  My optical densities were a little higher (or lower) than those in the manual that came with my kit. Why?

The optical density is affected by a number of physical conditions such as time ａnd temperature. We suggest that you shorten or lengthen the final incubation with substrate solution to compensate.

19.  What are the reasons for High Background?

1)     Improper Washing: Check volume of washing buffer reservoir ａnd make sure all recommended washing steps are performed.

2)     Contaminated Substrate: Make sure there is no contamination of the substrate with metal ions or oxidizing reagents, before use. Keep the extra substrate solution separately during the ELISA substrate development time. 【大鼠抗精子抗体(AsAb)ELISA试剂盒现货】

3)     Substrate exposed to light: Exposure to light may result in a blue color of the substrate. Keep solutions in the dark (vial) until ready to dispense into the plate.

4)     Wrong Incubation Times/Temperatures: Generally follow the test protocol regarding incubation times ａnd temperatures. However, if all wells are intensely ａnd equally colored with no intensity gradient observed in the standard dilution series, then it may be necessary to observe the substrate reaction as the color is developing, in order to stop the reaction sooner.

9242763  QS RGQ, Full Agreement "Repair service delivery QIAsymphony SP/AS: onsite

Service response time (repair) QIAsymphony: 48 hours/2 business days

Repair service delivery RGQ: send to Regional Repair Center

Service response time (repair) RGQ: 7-10 days turnaround time

Periodic inspection/maintenance: yes, 1/agreement year

Cost coverage for Repair Parts : yes, full coverage

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision RGQ: yes, included (provision time 2-3 days)

Transportation cost coverage: yes, full coverage"  CNY                             

9242547  SDS Canbus Cable, LC                 CNY                             

5000-1289        DML 2000 - Power Cord - 220V Switzerland             CNY                             

5000-1286        Fuse 2 AMP PKD 10, DML2                CNY                             

5000-1264        Cable Modem INST 9 PIN, DML2              CNY                             

5000-1507        Microplate Heater I - Fuse Inlet 3A 250V                  CNY                             

6000-00180      Plate Washer - Rinse Cap Assembly          CNY                             

6000-00179      Plate Washer - Wash Cap Assembly                   CNY                             

5000-1424        Brush Motor 2 Required, VTX2                  CNY                             

5000-1421        Block Support Back 099, VTX2                   CNY                             

5080-1000        Screw Caps                CNY                              【大鼠抗精子抗体(AsAb)ELISA试剂盒现货】

PUREGST2        GST-tag Purification ａnd Detection Kit 2                   CNY                             

5000-1411        Poteneiometer 100L OHM, VTX2             CNY                             

6000-5026        hc2 Sample Conversion Tubes 15mL                  CNY                             

L2127       hc2 High-Risk HPV DNA Test PI (CD-ROM)                CNY                             

12943       QIAGEN Plasmid Plus Midi Kit (25)  "25 QIAGEN Plasmid Plus Midi Columns, Extender Tubes, Reagents, Buffers, 25 QIAfilter Midi Cartridges

QIAGEN Plasmid Plus Kits enable ultrafast, large-scale plasmid purification of pure plasmid DNA. The use of a vacuum manifold allows purification of up to 12 (Mega ａnd Giga) or up to 24 (Midi ａnd Maxi) samples in parallel with low hands-on needs. Low elution volumes yield highly concentrated plasmid DNA for direct use without ethanol precipitation. QIAGEN Plasmid Plus Kits also feature a novel wash buffer for endotoxin reduction."    CNY                             

9240710  Filter, Pump, Jun-Air, Inlet/Outlet, AP               CNY                             

9240709  Filter, Pressure, 5 Micron, AP196/98                  CNY                             

9242094  QIAcube, Inspection Agreement     "Repair service delivery: not applicable

Service response time (repair): not applicable

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for repair parts: yes, for Periodic Inspection parts

Cost coverage for labor: yes, included for Periodic Maintenance

Cost coverage for travel: yes, included for Periodic Maintenance

Replacement system (Loaner) provision: not included

Transportation cost coverage: not applicable"        CNY                             

9242095  QIAxcel, Inspection Agreement                CNY                             

9241331  #QIAxcel, Full Agreement, no PM   The Full Agreement QIAxcel covers all travel, labor ａnd repair part costs related to an instrument repair for 1 year. Service response time is 2 working days.  CNY                             

9001772  careHPV test System                 CNY                             

275703     flocktype Salmonella Ab (5)               CNY                             

270045     cattletype BHV1 gB Ab (20)              CNY                             

274013     flocktype AIV Ab (5)          CNY                             

289993     virotype Tissue Lysis Reagent (250 ml)              CNY                             

281807     virotype CSFV RT-PCR Kit (480)                  CNY                              【大鼠抗精子抗体(AsAb)ELISA试剂盒现货】

273403     pigtype Toxoplasma Ab (5)                CNY                             

9011875  ZLV Vacuum Trap               CNY                             

9016874  Kit, Maint. Prot. Plasticware, BR UNIV            CNY                             

9232338  Cable, RS232, internal chain, BR8000               CNY                             

9238945  H-motor, BS15          CNY                             

9238927  Linear conductor, BS15             CNY                             

DMEM     DMEM     DMEM with High-Glucose - L-Glutamine ａnd Sodium Pyruvate      500 ml                                 

DMEM  DMEM  DMEM with High-Glucose - L-Glutamine ａnd Sodium Pyruvate     1L                                

DMEM    DMEM    DMEM with L-Glutamine - Sodium Pyruvate ａnd 25 mM HEPES     500 ml                                 

DMEM  DMEM  DMEM with High-Glucose - L-Glutamine - Sodium Pyruvate ａnd 25 mM HEPES     500 ml                                 

DMEM-prf        DMEM-prf        DMEM with High Glucose - L-Glutamine ａnd Sodium Pyruvate - phenol red-free  1L                                

Ham＇s F-10       Ham＇s F-10       F-10 with L-Glutamine ａnd 25 mM HEPES      500 ml                                 

Ham＇s F-12       Ham＇s F-12       F-12 with L-Glutamine ａnd 25 mM HEPES      500 ml                                 

DMEM/F-12     DMEM/F-12     DMEM/F-12 with L-Glutamine        500 ml                                  【大鼠抗精子抗体(AsAb)ELISA试剂盒现货】

DMEM/F-12    DMEM/F-12    DMEM/F-12 with L-Glutamine ａnd 15 mM HEPES          500 ml                                 

RPMI 1640       RPMI 1640       RPMI  1640 without L-Glutamine - with 25 mM HEPES        500 ml                                 

RPMI 1640       RPMI 1640       RPMI  1640 with L-Glutamine ａnd 25 mM HEPES          500 ml                                 

IMDM    IMDM    MDM with L-Glutamine ａnd 25 mM HEPES; without alpha-Thioglycerol - 2-mercaptoethanol   500 ml                                 

rhSCF        重组人干细胞因子         Recombinant Human Stem Cell Factor    2µg                             

rhG-CSF    重组人粒细胞集落刺激因子         Recombinant Human Granulocyte Colony Stimulating Factor         2µg                             

rhGM-CSF         重组人粒细胞巨噬细胞集落刺激因子         Recombinant Human Granulocyte Macrophage Colony Stimulating Factor         5µg                                     

rhM-CSF   重组人巨噬细胞集落刺激因子     Recombinant Human Macrophage Colony Stimulating Factor        2µg                             

rhTNF-α 重组人肿瘤坏死因子-α        Recombinant Human Tumor Necrosis Factor-alpha         10µg                                    

rhTNF-α-His    重组人肿瘤坏死因子-α,His Recombinant Human Tumor Necrosis Factor-alpha,His  10µg                                    

rhTNF-α Vari 重组人肿瘤坏死因子-αVariant Recombinant Human Tumor Necrosis Factor- alpha Variant          10µg                                    

rhBAF       重组人B细胞活化因子         Recombinant Human B Cell Activating Factor         5µg                                     

rhOSM      重组人抑瘤素-M    Recombinant Human Oncostatin-M        2µg                             

rhOSM 209a.a.         重组人抑瘤素-M209a.a.        Recombinant Human Oncostatin-M 209a.a.  2µg                             

rhAK1-3    重组人血管抑素K1-3     Recombinant Human Angiostatin K1-3   10µg                                    

rhEndostatin    重组人血管内皮抑素     Recombinant Human Endostatin     2µg                              【大鼠抗精子抗体(AsAb)ELISA试剂盒现货】

rhaFGF     重组人成纤维细胞生长因子－－酸性       Recombinant Human Fibroblast Growth Factor- acidic  10µg                                    

rhbFGF     重组人成纤维细胞生长因子－－碱性       Recombinant Human Fibroblast Growth Factor-basic    10µg                                    

rhKGF-1    重组人角质细胞生长因子-1 Recombinant Human Keratinocye Growth Factor-1        2µg                             

rhFGF-9    重组人成纤维细胞生长因子-9      Recombinant Human Fibrobalst Growth Factor-9  5µg                                     

rhKGF-2    重组人角质细胞生长因子-2 "Recombinant Human Keratinocye Growth Factor-2

"       5µg                             

rhFGF-19  重组人成纤维细胞生长因子-19   "Recombinant Human Fibrobalst Growth Factor-19

"       5µg                                     

rhFGF-21  重组人成纤维细胞生长因子-21   "Recombinant Human Fibrobalst Growth Factor-21

"       5µg