检测标本：血清,血浆,尿液,胸腹水,脑脊液,细胞培养上清,组织匀浆等

检测方法：ELISA

检测类型：酶联免疫夹心法

产品的用途：仅供科研究课题使用

价格及详细资料：齐一生物销售：021－6034 8496；181214 53965；173021 04490；.齐一生物科技（上海）有限公司提供的ELISA试剂盒受到了广大科研单位的一致肯定和认同.大品牌保证,价格公道,倾力为国内外科研院校实验室提供*优质的产品.若有需要,我司将竭诚为您服务！本试剂盒用于测定血清,血浆及相关液体样本中含量.

【大鼠白介素16(IL-16)ELISA试剂盒厂家】实验原理：

   本试剂盒应用双抗体夹心法测定标本中该产品水平.用纯化的本产品抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入本产品抗原,再与HRP标记的本产品抗体结合,形成抗体-抗原-酶标抗体复合物,经过彻底洗涤后加底物TMB显色.TMB在HRP酶的催化下转化成蓝色,并在酸的作用下转化成*终的黄色.颜色的深浅和样品中的本产品呈正相关.用酶标仪在450nm波长下测定吸光度（OD值）,通过标准曲线计算样品中该产品浓度.

试剂盒组成：

齐一生物销售：021－6034 8496；181214 53965；173021 04490

【大鼠白介素16(IL-16)ELISA试剂盒厂家】标本要求：

1．标本采集后尽早进行提取,提取按相关文献进行,提取后应尽快进行实验.若不能马上进行试验,可将标本放于-20℃保存,但应避免反复冻融

2．不能检测含NaN3的样品,因NaN3抑制辣根过氧化物酶的（HRP）活性.

操作步骤：

1.     标准品的稀释与加样：在酶标包被板上设标准品孔10孔,在、第二孔中分别加标准品100μl,然后在、第二孔中加标准品稀释液50μl,混匀；然后从孔、第二孔中各取100μl分别加到第三孔和第四孔,再在第三、第四孔分别加标准品稀释液50μl,混匀；然后在第三孔和第四孔中先各取50μl弃掉,再各取50μl分别加到第五、第六孔中,再在第五、第六孔中分别加标准品稀释液50ul,混匀；混匀后从第五、第六孔中各取50μl分别加到第七、第八孔中,再在第七、第八孔中分别加标准品稀释液50μl,混匀后从第七、第八孔中分别取50μl加到第九、第十孔中,再在第九第十孔分别加标准品稀释液50μl,混匀后从第九第十孔中各取50μl弃掉.（稀释后各孔加样量都为50μl,浓度分别为1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L）. 【大鼠白介素16(IL-16)ELISA试剂盒厂家】

2.     加样：分别设空白孔（空白对照孔不加样品及酶标试剂,其余各步操作相同）、待测样品孔.在酶标包被板上待测样品孔中先加样品稀释液40μl,然后再加待测样品10μl（样品*终稀释度为5倍）.加样将样品加于酶标板孔底部,尽量不触及孔壁,轻轻晃动混匀.

3.     温育：用封板膜封板后置37℃温育30分钟.

4.     配液：将30（48T的20倍）倍浓缩洗涤液用蒸馏水30（48T的20倍）倍稀释后备用.

5.     洗涤：小心揭掉封板膜,弃去液体,甩干,每孔加满洗涤液,静置30秒后弃去,如此重复5次,拍干.

6.     加酶：每孔加入酶标试剂50μl,空白孔除外.

7.     温育：操作同3.

8.     洗涤：操作同5.

9.     显色：每孔先加入显色剂A50μl,再加入显色剂B50μl,轻轻震荡混匀,37℃避光显色15分钟.

10. 终止：每孔加终止液50μl,终止反应（此时蓝色立转黄色）.

11. 测定：以空白空调零,450nm波长依序测量各孔的吸光度（OD值）. 测定应在加终止液后15分钟以内进行.

注意事项：

1． 试剂盒从冷藏环境中取出应在室温平衡15-30分钟后方可使用,酶标包被板开封后如未用完,板条应装入密封袋中保存.

2． 浓洗涤液可能会有结晶析出,稀释时可在水浴中加温助溶,洗涤时不影响结果. 【大鼠白介素16(IL-16)ELISA试剂盒厂家】

3． 各步加样均应使用加样器,并经常校对其准确性,以避免试验误差.一次加样时间控制在5分钟内,如标本数量多,推荐使用排抢加样.

4． 请每次测定的同时做标准曲线,做复孔.如标本中待测物质含量过高（样本OD值大于标准品孔孔的OD值）,请先用样品稀释液稀释一定倍数（n倍）后再测定,计算时请*后乘以总稀释倍数（×n×5）.

5． 封板膜只限一次性使用,以避免交叉污染.

6． 底物请避光保存.

7． 严格按照说明书的操作进行,试验结果判定必须以酶标仪读数为准.

8． 所有样品,洗涤液和各种废弃物都应按传染物处理.

9． 本试剂不同批号组分不得混用.

10. 如与英文说明书有异,以英文说明书为准.

计算：

以标准物的浓度为横坐标,OD值为纵坐标,   

在坐标纸上绘出标准曲线,根据样品的OD     

值由标准曲线查出相应的浓度；再乘以稀释     

倍数；或用标准物的浓度与OD值计算出标     

准曲线的直线回归方程式,将样品的OD值     

代入方程式,计算出样品浓度,再乘以稀释

倍数,即为样品的实际浓度.

试剂盒性能：

1.样品线性回归与预期浓度相关系数R值为0.95以上.

2.批内与批见应分别小于9%和11%    

保存条件及有效期：

1.试剂盒保存；2-8℃.

2．有效期：6个月

800328     Miniprep, PCR purification (Easy-Read)            CNY                             

132109     EasyXtal 15-Well DG-Tool X-Seal (100)     "100 empty 15-well crystallization plates with dropguard, X-Seal crystallization supports

For manual setup of hanging ｄrop crystallization experiments."    CNY                             

132101     NeXtal DWBlock Adhesive Foils                 CNY                             

132045     NeXtal Evolution µplate (10)             CNY                             

338172     RT2 Calibration Plate A             CNY                             

338125     384EZload Covers (4)                 CNY                              【大鼠白介素16(IL-16)ELISA试剂盒厂家】

762431     PAXgene Blood RNA MDx Kit (4)               CNY                             

203745     QIAGEN Fast Cycling PCR Kit (1000)        For 1000 x 20 µl reactions: 10 x 1 ml Fast Cycling Master Mix (2x), RNase-free water, 10 x CoralLoad Fast Cycling Dye, 5 x Q-Solution       CNY                             

203609     HotStarTaq Plus DNA Polymerase (25000)       100 x 250 units HotStarTaq Plus DNA Polymerase, 10x PCR Buffer, 10x CoralLoad PCR Buffer, 100 x 2.0ml Q-Solution, 25 mM MgCl2         CNY                             

201205     Taq DNA Polymerase (1000)    "4 x 250 units Taq DNA Polymerase, 10x PCR Buffer,* 5x Q-Solution, 25 mM MgCl2

NEW! Now with 10x CoralLoad PCR Buffer

Taq DNA Polymerase is supplied with CoralLoad PCR Buffer, which has all of the advantages of QIAGEN PCR Buffer but can also be used to directly load the PCR reaction onto an agarose gel without the need to add a gel loading buffer. CoralLoad PCR Buffer provides the same high PCR specificity ａnd minimal reaction optimization as conventional QIAGEN PCR Buffer. Additionally, it contains two marker dyes: an orange dye ａnd a red dye, that facilitate estimation of DNA migration distance ａnd optimization of agarose gel run time."         CNY                             

180850     GeneRead qPCR FAST SYBR Green/ROX MM (2)               CNY                             

159300     QIAcard FTA Purification Reagent (500ml)                CNY                             

158928     Lytic Enzyme Solution (650 µl)          CNY                             

9240549  Imbalance PCB, Eppendorf, AP196/98               CNY                             

9240547  Motor, 230V, Eppendorf, AP196/98           CNY                             

9240528  Cable, Motor, Axis #5, TR, AP98                CNY                             

9240047  Thermostate unit, 120V, 1.04          CNY                             

9020029  Cable RC-3034, RGQ                  CNY                             

9020017  Oil for PM, EZ1                   CNY                             

9020016  Cable harness, VacuumStation PCB, CX1          CNY                             

9019938  Opto switch, P-axis, 1 ch. , Cx2                  CNY                             

9019486  HEPA filter, replacement cartridge, Cxx           CNY                              【大鼠白介素16(IL-16)ELISA试剂盒厂家】

9238861  BR 8000, Premium Agreement        The Premium Cover Agreement 8000 provides full service coverage for the BioRobot 8000. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 24 hours (1 working day).   CNY                             

9238855  BR MDx, Basic Agreement      The Basic Cover Agreement MDx provides full service coverage for the BioRobot MDx. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.     CNY                            

9238850  EZ1, Basic Agreement     The Basic Cover Agreement EZ1 provides full service coverage for the BioRobot EZ1. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.      CNY                             

9241226  Barcode Reader, EZ1a              CNY                             

9238812  Rectangular carrier, undrilled          CNY                             

9019467  PLATE GLASS SHORT SET GS              CNY                             

9019465  Pads, D=10mm, white (85), Cxx                CNY                             

9019459  Transformer (115V), HS            CNY                             

9233429  Valve, magnet, dispenser, BR8000           CNY                             

9233334  Solenoid Valve, 5-fold                CNY                             

9237332  Install, BR plus 2 integr. w. scheduling     Installation ａnd training, BioRobot System including two approved integration devices with scheduling   CNY                             

L-Glu         左旋谷氨酰胺溶液        L-Glutamine Solution, 200 mM        100 ml                                 

NEAA        100X非必需氨基酸        100X Non-Essential Amino Acids      100ml                                  

HGL PCR  人胚层检测试剂盒         Human Germ Layer Detection Kit   50 reactions                               

Ored         油红O染色试剂盒         Oil Red O Staining Kit       100 ml                                

StemDS    人胚胎干细胞分离液     StemDS® Human Embryonic Stem Cell Dissociation Solution  100 ml                                

BPF  牛血浆纤维粘连蛋白     Bovine Plasma Fibronectin       1 mg                                    

HCF  人细胞纤连蛋白     Human Cellular Fibronectin     100 μg                             

BPV  牛血浆玻连蛋白     Bovine Plasma Vitronectin    100 μg                             

rHSA         重组人血清白蛋白         "Recombinant Human Serum Albumin

"       1g                                

BPF  牛血浆纤维粘连蛋白     Bovine Plasma Fibronectin       1 mg                                    

CCCSCK     胶原I-细胞培养表面包被试剂盒        Collage I-Cell Culture Surface Coating Kit         10 mg                                  

PLL   多聚赖氨酸   Poly-L-Lysine 1 mg/ml       1 ml                            

PLL   多聚赖氨酸   Poly-L-Lysine 10 mg/ml    1 ml                             【大鼠白介素16(IL-16)ELISA试剂盒厂家】

STEMium 人胚胎干细胞无血清培养基         Human Pluripotent Stem Cell Growth Medium       500 ml                                 

STEMium-XF     无异种人胚胎干细胞生长培养基         Xeno-free Human Pluripotent Stem Cell Growth Medium       500 ml                                 

STEMium-ACF  无动物成分的人胚胎干细胞生长培养基     Animal Component-free Human Pluripotent Stem Cell Growth Medium         500 ml                                 

StemDS    人胚胎干细胞分离液     StemDS® Human Embryonic Stem Cell Dissociation Solution  100 ml                                

StemCryo 人胚胎干细胞冻存液     Human Pluripotent Stem Cell Cryopreservation Medium        50ml                                    

StemCryo 人胚胎干细胞冻存液     Human Pluripotent Stem Cell Cryopreservation Medium,       5 x 10mL                                     

MEF 小鼠胚胎成纤维细胞     Mouse Embryonic Fibroblasts 5 x 10^5 cells/vial                                

MEF-mt    小鼠胚胎成纤维细胞－－处理  Mouse Embryonic Fibroblasts-mitomicine treated 5 x 10^5 cells/vial                                

MEF-mt    小鼠胚胎成纤维细胞－－CD1小鼠C处理        Mouse Embryonic Fibroblasts- Mitomycin C treated from CD1 mouse 1 x 10^6 cells/vial                              

MEF-mt    小鼠胚胎成纤维细胞－－CF1小鼠C处理         Mouse Embryonic Fibroblasts from CF1 mouse      1 x 10^6 cells/vial                                

MEF-mt    小鼠胚胎成纤维细胞－－CF1小鼠C处理         Mouse Embryonic Fibroblasts-Mitomycin C treated from CF1 mouse    1 x 10^6 cells/vial                              

HDF-f-mt 人真皮成纤维细胞-胎儿C处理    Human Dermal Fibroblasts-Fetal-Mitomycin C treated  1 x 10^6 cells/vial                              

MEF-cm   小鼠胚胎成纤维细胞培养基         Mouse Embryonic Fibroblasts-Conditioned Medium        100 ml                                

bFGF-std MEF-cm     碱性成纤维细胞生长因子刺激的小鼠胚胎成纤维细胞条件培养基         bFGF-Stimulated Mouse Embryonic Fibroblasts Conditioned Medium     100ml                                  

ULBC plates      干细胞超低结合培养板         Ultra-Low Binding Culture Plate      6-well, 1 plate                                   

ULBC plates      超低结合培养板（6孔）      Ultra-Low Binding Culture Plate (6-well)         "6-well, 4 plates

"                                   【大鼠白介素16(IL-16)ELISA试剂盒厂家】

ULBC plate       超低结合培养板（24孔）    Ultra-Low Binding Culture Plate (24-well)      "24-well, 1 plate

"                                  

ULBC plates      超低结合培养板（24孔）    Ultra-Low Binding Culture Plate (24-well)      "24-well, 4 plates

"