1. 酶标仪(450nm 波长滤光片)

2. 高精度移液器，EP 管及一次性吸头

3. 37℃恒温箱，双蒸水或去离子水

4. 吸水纸

【友情提示】：本产品仅供科研研究使用，不得用于人体临床直接检测。避免给您带来不必要的损失，请仔细阅读购买说明！【大鼠巨噬细胞炎性蛋白2(MIP-2)ELISA试剂盒现货】

ELISA应用：

ELISA检测试剂盒、ELISAKit、酶联免疫试剂盒、酶联免疫吸附测定试剂盒、酶联免疫分析试剂盒、酶免试剂盒等,比较常见的叫法是ELISA检测试剂盒、酶联免疫吸附测定试剂盒等ELISA试剂盒自从60-70年代问世以来,得到全世界科研工作者的认可及推崇,在欧美及中国获得很大的推广,尤其是国内生化领域的长足发展.Elisa生物试验是一种敏感性高,特异性强,重复性好的实验诊断方法.由于其试剂稳定、易保存,操作简便,结果判断较客观等因素,已广泛应用在免疫学检验的各领域中.ELISA检测试剂盒适用于体外定性检测人血清或血浆中的抗人类戊型肝炎（HEV）病毒IgM抗体ELISA检测

【大鼠巨噬细胞炎性蛋白2(MIP-2)ELISA试剂盒现货】齐一生物销售：021－6034 8496；181214 53965；173021 04490  web：www.qiyibio.com

试剂盒安全性提示：

1.工作人员实验中避免直接接触终止液和底物A、B。一旦接触到这些液体，请尽快用水冲洗。

2.实验中不要吃喝、抽烟或使用化妆品。

3.不要用嘴吸取试剂盒里的任何成份。

样本保存条件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6个月

3.-50℃以下的冰箱，可以放一年

【大鼠巨噬细胞炎性蛋白2(MIP-2)ELISA试剂盒现货】ELISA实验原理

试剂盒是固相夹心法酶联免疫吸附实验（ELISA）.已知浓度的标准品、未知浓度的样品加入微孔酶标板内进行检测.先将生物素标记的抗体同时温育.洗涤后,加入亲和素标记过的HRP.再经过温育和洗涤,去除未结合的酶结合物,然后加入底物A、B,和酶结合物同时作用.产生颜色.颜色的深浅和样品中的浓度呈比例关系.

免疫测定技术的基础在于抗原抗体之间的特异结合反应,所以任何优质的诊断试剂离不开优质的原料,如抗原抗体,酶等.以前用于免疫测定的抗原通常为各种纯化抗原,而抗体则为纯化抗原免疫动物后获得的多克隆抗体和使用杂交瘤技术得到的单克隆抗体,而抗原或抗体的标记物如酶标结合物则通过各种化学合成方法制备.近年来,随着分子生物学的发展,使用基因工程方法制备各种特殊的抗原或抗体及其酶结合物等免疫测定试剂几成为现实的新一代试剂,各种新型使用的测定方法也不断出现.

HBsAg试剂特点（检测模式：临床抗体夹心法）：包被抗体为山羊多抗.多抗具有高亲和性和对抗原各种表位的反应性.酶表抗体为与HBsAg有不同结合位点的鼠复合单位,可测得HBsAg变异样品,提高检测的特异性（99.98%）提高检测的灵敏度,应用ParlEhrich学说（PEI）HBsAg标准品,对ad标准品的灵敏度为0.05ng/ml对ay标准品灵敏度为0.025ng/ml

ELISA检测试剂盒应用定性夹心免疫检测技术,用合成的HEV多肽抗原包被微孔板板条,这些多肽是中国型HEV毒株核心氨基酸序列中抗原性很强的肽段,分别来自于该毒株的开放阅读框2和开放阅读框3.将样品或标准品加入孔中并孵育,如果其中存在HEVIgM抗体,这些抗体就会与HEV的多肽抗原结合,并固定在上面,洗板除去其它非特异性抗体和样品中的其它成份.然后加入羊抗人IgM-HRP（辣根过氧化物酶）酶结合物,经第二次孵育后,酶结合物就会与次孵育结合上的HEVIgM抗体相结合,洗板除去未结合的酶结合物,加入TMB底物溶液,在第三次孵育时会发生酶-底物反应,只有那些含有HEVIgM抗体和酶结合物所形成的复合物的孔才会发生颜色变化,加入硫酸溶液终止酶和底物间的反应,并在波长:450nm处测量O.D.值,按照本HEVIgM抗体elisa试剂盒的测试标准,O.D.值大于或等于Cut-Off值的样品被认为是初试阳性.

以上资料仅供参考了解，不供于论文写作

【大鼠巨噬细胞炎性蛋白2(MIP-2)ELISA试剂盒现货】齐一生物专业为您提供高品质，高灵敏度.高质量的酶联免疫试剂盒，齐一生物长期经营ELISA试剂盒（免费代测）及抗体、分子学生物试剂盒、金标法检测试剂盒、比色法检测试剂盒、细胞菌株、培养基、细胞因子、生化试剂、耗材等生物试剂产品。齐一生物销售：021－6034 8496；181214 53965；173021 04490欢迎选购！

30004   Strep-Tactin Superflow Plus (10 ml)     CNY            

28706   QIAquick Gel Extraction Kit (250)   250 QIAquick Spin Columns, Buffers, Collection Tubes (2 ml)    CNY            

9013181 Tubing, pump outlet, DISU, BR8000       CNY            

9011481 BGR Greiferkopf Plato 1xxx      CNY            

6000-1240U  Microplate Heater I, 240V       CNY            

9011334 Arm Mechanism, RCS1     CNY            

330514  RT² SYBR Green Fluor qPCR Mastermix (6)     CNY            

9011092 Mounting plate, power supplies, BR8000      CNY            

150023  REPLI-g Mini Kit (25)   DNA Polymerase, Buffers, ａnd Reagents for 25 x 50 µl whole genome amplification reactions (typical yield 10 µg per reaction)    CNY            

9241806 Pyro Q24, Installation  Installation ａnd Introductory Training PyroMark is an on-site service, conducted by a QIAGEN Service Specialist. It covers the installation ａnd commissioning of the instrument ａnd the handling of the PyroMark systems hardware. It trains the user on the operation of the PyroMark software ａnd the setup ａnd execution of a pyrosequencing run. After the training the user will be able to prepare samples, set-up ａnd run the instrument, analyse the results ａnd design new assays as well as troubleshoot new assays ａnd perform basic maintenance on the sample preparation tool ａnd instrument. All travel ａnd labor costs ａnd well as material for the demonstration run are included. The service is documented in accordance with GMP/GLP requirements    CNY            

9241177 Power switch, 6A, 250VAC, QX        CNY            

9241147 Transport  lock, X axis, M48        CNY            

9241032 Tube insert, 2.0 ml,samp.carr., Qsym        CNY            

9240986 PCB, main, cooling device, Qsym     CNY             【大鼠巨噬细胞炎性蛋白2(MIP-2)ELISA试剂盒现货】

9240981 Slide block, sample tracking, Qsym      CNY            

9240974 Carrier locking, sample tracking, Qsym      CNY            

9240967 Sample tracking, Qsym       CNY            

9240963 Drop catcher, pipetting system, Qsym        CNY            

9240944 Motor, xy, portal, Qsym     CNY            

9234853 Modem, analoge ELSA     CNY            

9240763 Analog Module, F2-04AD-1, AP96      CNY            

9241376 Upgrade, Autopure LS Software v3.x  The “Upgrade, Autopure LS Software v3.x” is an on-site service for the software upgrade to the latest Autopure LS software version (currently version 3.1), the verification of the instrument performance (including the setting of database parameter), ａnd a user training. The product is available for Autopure LS instruments (Version AP-98) with a current software version lower or equal to V2.4 ａnd is performed by a QIAGEN service specialist.    CNY            

9241764 QIAgility, Premium Agreement    "Repair service delivery: onsite

Service response time (repair): 24 hours/1 business day

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for repair parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) or PC repair provision: not included

Transportation cost coverage: not applicable"   CNY            

9241757 Certification, QIAsymphony      CNY            

9241464 USB Memory Stick 2GB, Qsym      CNY            

9241370 Power supply, EZ1a      CNY            

9241363 Ribbon cable, CPU board, EZ1a       CNY            

9240756 Downtube, 500 ml Bottle, AP98       CNY            

9240748 Belt, Dispense Head, AP     CNY            

9243543 BR Universal, PM Service    "Service response time (repair): not applicable

Periodic inspection/maintenance: yes

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts : not included

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage"   CNY             【大鼠巨噬细胞炎性蛋白2(MIP-2)ELISA试剂盒现货】

9243308 HC2 Systems Training – In House    HC2 In house, 3 day course trains users on the HC2 system (manual or automated) ａnd includes participate travel, lodging, meals, materials ａnd PACE continuing education credits.    CNY            

9242861 QIArent&go – Rent out fee QIAxcel Adv.     CNY            

9242544 Power Input Module, Eppendorf, AP196/98     CNY            

    神经元细胞  5 ×105 1ml            

    神经胶质细胞    5 × 105    1ml            

    海马神经元细胞  5 × 105    1ml            

    脑微血管内皮细胞    5 × 105    1ml            

    脑成纤维细胞    5 × 105    1ml             【大鼠巨噬细胞炎性蛋白2(MIP-2)ELISA试剂盒现货】

    神经小胶质细胞  5 × 105    1ml            

    雪旺氏细胞  5 × 105    1ml            

    食管癌组织源性原代细胞  5 × 105/1ml                   

    胃癌组织源性原代细胞    5 × 105/1ml                   

    肝癌组织源性原代细胞    5 × 105/1ml                   

    结肠癌组织源性原代细胞  5 × 105/1ml                   

    直肠癌组织源性原代细胞  5 × 105/1ml                   

    肺癌组织源性原代细胞    5 × 105/1ml                   

    胆道癌组织源性原代细胞  5 × 105/1ml                   

    肾癌组织源性原代细胞    5 × 105/1ml                   

    膀胱癌组织源性原代细胞  5 × 105/1ml                   

    胰腺癌组织源性原代细胞  5 × 105/1ml                   

    甲状腺癌组织源性原代细胞    5 × 105/1ml                   

    前列腺癌组织源性原代细胞    5 × 105/1ml                   

    乳腺癌组织源性原代细胞  5 × 105/1ml                   

    卵巢癌组织源性原代细胞  5 × 105/1ml                    【大鼠巨噬细胞炎性蛋白2(MIP-2)ELISA试剂盒现货】

    子宫癌组织源性原代细胞  5 × 105/1ml                   

    宫颈癌组织源性原代细胞  5 × 105/1ml                   

    皮肤癌组织源性原代细胞  5 × 105/1ml                   

    胶质瘤组织源性原代细胞  5 × 105/1ml                   

    髓母细胞瘤组织源性原代细胞  5 × 105/1ml                   

    胃癌组织源性原代成纤维细胞  5 × 105/1ml                   

    肺微血管内皮细胞    5 × 105    1ml            

    肺大动脉内皮细胞    5 × 105    1ml            

    肺大动脉平滑肌细胞  5 × 105    1ml            

    肺大静脉内皮细胞    5 × 105    1ml