1. 酶标仪(450nm 波长滤光片)

2. 高精度移液器，EP 管及一次性吸头

3. 37℃恒温箱，双蒸水或去离子水

4. 吸水纸

【大鼠血管内皮细胞生长因子受体2(VEGFR-2/Flk-1)ELISA试剂盒现货】齐一生物【友情提示】：本产品仅供科研研究使用，不得用于人体临床直接检测。避免给您带来不必要的损失，请仔细阅读购买说明！

ELISA应用：

ELISA检测试剂盒、ELISAKit、酶联免疫试剂盒、酶联免疫吸附测定试剂盒、酶联免疫分析试剂盒、酶免试剂盒等,比较常见的叫法是ELISA检测试剂盒、酶联免疫吸附测定试剂盒等ELISA试剂盒自从60-70年代问世以来,得到全世界科研工作者的认可及推崇,在欧美及中国获得很大的推广,尤其是国内生化领域的长足发展.Elisa生物试验是一种敏感性高,特异性强,重复性好的实验诊断方法.由于其试剂稳定、易保存,操作简便,结果判断较客观等因素,已广泛应用在免疫学检验的各领域中.ELISA检测试剂盒适用于体外定性检测人血清或血浆中的抗人类戊型肝炎（HEV）病毒IgM抗体ELISA检测

【大鼠血管内皮细胞生长因子受体2(VEGFR-2/Flk-1)ELISA试剂盒现货】齐一生物科技欢迎您订购咨询，齐一生物销售：021－6034 8496；181214 53965；173021 04490

齐一生物供应的ELISA试剂盒安全性提示：

1.工作人员实验中避免直接接触终止液和底物A、B。一旦接触到这些液体，请尽快用水冲洗。

2.实验中不要吃喝、抽烟或使用化妆品。

3.不要用嘴吸取试剂盒里的任何成份。

样本保存条件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6个月

3.-50℃以下的冰箱，可以放一年

【大鼠血管内皮细胞生长因子受体2(VEGFR-2/Flk-1)ELISA试剂盒现货】齐一生物供应的ELISA试剂盒ELISA实验原理

试剂盒是固相夹心法酶联免疫吸附实验（ELISA）.已知浓度的标准品、未知浓度的样品加入微孔酶标板内进行检测.先将生物素标记的抗体同时温育.洗涤后,加入亲和素标记过的HRP.再经过温育和洗涤,去除未结合的酶结合物,然后加入底物A、B,和酶结合物同时作用.产生颜色.颜色的深浅和样品中的浓度呈比例关系.

免疫测定技术的基础在于抗原抗体之间的特异结合反应,所以任何优质的诊断试剂离不开优质的原料,如抗原抗体,酶等.以前用于免疫测定的抗原通常为各种纯化抗原,而抗体则为纯化抗原免疫动物后获得的多克隆抗体和使用杂交瘤技术得到的单克隆抗体,而抗原或抗体的标记物如酶标结合物则通过各种化学合成方法制备.近年来,随着分子生物学的发展,使用基因工程方法制备各种特殊的抗原或抗体及其酶结合物等免疫测定试剂几成为现实的新一代试剂,各种新型使用的测定方法也不断出现.

HBsAg试剂特点（检测模式：临床抗体夹心法）：包被抗体为山羊多抗.多抗具有高亲和性和对抗原各种表位的反应性.酶表抗体为与HBsAg有不同结合位点的鼠复合单位,可测得HBsAg变异样品,提高检测的特异性（99.98%）提高检测的灵敏度,应用ParlEhrich学说（PEI）HBsAg标准品,对ad标准品的灵敏度为0.05ng/ml对ay标准品灵敏度为0.025ng/ml

ELISA检测试剂盒应用定性夹心免疫检测技术,用合成的HEV多肽抗原包被微孔板板条,这些多肽是中国型HEV毒株核心氨基酸序列中抗原性很强的肽段,分别来自于该毒株的开放阅读框2和开放阅读框3.将样品或标准品加入孔中并孵育,如果其中存在HEVIgM抗体,这些抗体就会与HEV的多肽抗原结合,并固定在上面,洗板除去其它非特异性抗体和样品中的其它成份.然后加入羊抗人IgM-HRP（辣根过氧化物酶）酶结合物,经第二次孵育后,酶结合物就会与次孵育结合上的HEVIgM抗体相结合,洗板除去未结合的酶结合物,加入TMB底物溶液,在第三次孵育时会发生酶-底物反应,只有那些含有HEVIgM抗体和酶结合物所形成的复合物的孔才会发生颜色变化,加入硫酸溶液终止酶和底物间的反应,并在波长:450nm处测量O.D.值,按照本HEVIgM抗体elisa试剂盒的测试标准,O.D.值大于或等于Cut-Off值的样品被认为是初试阳性. 【大鼠血管内皮细胞生长因子受体2(VEGFR-2/Flk-1)ELISA试剂盒现货】

以上资料仅供参考了解，不供于论文写作

齐一生物专业为您提供高品质，高灵敏度.高质量的酶联免疫试剂盒，齐一生物长期经营ELISA试剂盒（免费代测）及抗体、分子学生物试剂盒、金标法检测试剂盒、比色法检测试剂盒、细胞菌株、培养基、细胞因子、生化试剂、耗材等生物试剂产品。齐一生物销售：021－6034 8496；181214 53965；173021 04490

9236364  Sheath Filter             CNY                             

9232933  Microplate Reader(Agglutination)Module                CNY                             

9232916  Card, liquid detection 1.02                CNY                             

9232910  Board, Z1 motor                CNY                             

9236351  Tubing, Valve to injection needle              CNY                             

9236326  Sheath Pressure Sensor             CNY                             

9235760  Seal, O-ring, 3mm             CNY                             

9230926  Motor Connecting Ground Cable              CNY                             

9230545  Wanne fuer Abschirmung Plato 1xxx                 CNY                             

9235700  Power Cable 24V, RCS1             CNY                             

9230291  Tubing Guide (periscope)          CNY                             

211011     QuantiTect Virus Kit (50)          CNY                              【大鼠血管内皮细胞生长因子受体2(VEGFR-2/Flk-1)ELISA试剂盒现货】

979017     MOTT 16S Primer 2 For Sequencing                  CNY                             

59824       EpiTect Fast DNA Bisulfite Kit (50)            CNY                             

206246     Type-it Microsatellite PCR Kit (2000)                 CNY                             

9012742  Syringe pump outlet tubing (4.7 m)                   CNY                             

9012736  Peristaltic pump outlet tubing (1.0 m)              CNY                             

9012725  Syringe pump outlet for Plato           CNY                             

979001     PyroMark Q96 Plate (100)        "For sample analysis of DNA template prepared with magnetic beads for ID.

Magnetic bead technology instead of vacuum prep tool is used here"   CNY                             

9012706  Base, auto vac, BR8000  This is the robovac base for the BR8000 I. There are 5 contacts.   CNY                             

133240     NeXtalStock Sodium hydroxide (200)                 CNY                             

133233     NeXtalStock Sodium cacodyl. pH 7.5 (100)                CNY                             

9002195  RGQ 2plex, PC, PrioPLUS, basic, no PM   "RGQ 2plex, PC Instrument

Installation of RGQ 2plex & Introductory training

Warranty extension to 3 yrs

Repair Center service, response 7-10 days

No Loaner available

No preventive Maintenance

Priority service provision over non-contract customers

Documented installation & repair report

Unlimited number of repair service

All cost covered (Shipping, labor, parts)"          CNY                             

9002039  Rotor-Gene Q MDx 5plex HRM, PrioPLUS       "Rotor-Gene Instrument

Installation of Rotor-Gene & Introductory training

Warranty extension to 3 yrs

Regional Repair Center service, 7-10 day turn around time

Loaner available in 2 days*

Annual Inspections (1 PM per yearr)

Priority service provision over non-contract customers

Documented installation & PM report

Unlimited number of repair service

All cost covered (shipping, labor, parts)" CNY                             

59436       EpiTect MethyLight PCR Kit (200)             CNY                             

971540     therascreen® UGT1A1 Pyro Kit (24)                  CNY                             

133217     NeXtalStock MOPS pH 7.5 (200)                CNY                             

51504       QIAamp DNA Stool Mini Kit (50)      For 50 DNA preps: 50 QIAamp Mini Spin Columns, QIAGEN Proteinase K, InhibitEX tablets, Buffers, Collection Tubes (2 ml)  CNY                              【大鼠血管内皮细胞生长因子受体2(VEGFR-2/Flk-1)ELISA试剂盒现货】

9002013  Rotor-Gene Q MDx 2plex HRM System            CNY                             

970032     PyroMark Q24 CpG MGMT (4 x 24) For quantification of methylation level in region 17-39 of exon 1 of the MGMT gene.         CNY                             

922920     LiquiChip Filter Microplates (10)               CNY                             

292023     mericon Cattle (24)           CNY                             

292013     mericon Pig Kit (24)          CNY                             

QY-17442 Anti-PP-2A（protein phosphatase 2A catalytic subunit）蛋白质磷酸酶-2A抗体                                                      

QY-17452 Anti-PP2Ac磷酸酯酶-2Ac抗体                                                    

QY-17462 Anti-PPAR-delta (peroxisome proliferator-activated receptor;PPAR-beta)D型-过氧化酶活化增生受体抗体                                                  

QY-17472 Anti-PPARγ (peroxisome proliferator-activated receptor-gamma)过氧化酶活化增生受体γ抗体                                               

QY-17482 Anti-PKB (Akt1/PKB)蛋白激酶B（兔来源抗体）                                               

QY-17492 anti-P-AKT1/PKB1/2/3（Thr473）磷酸化蛋白激酶B抗体（丝氨酸磷酸化位点：473）                                                

QY-17502 anti-PKN2 (protein kinase N2)蛋白激酶N2抗体                                                  【大鼠血管内皮细胞生长因子受体2(VEGFR-2/Flk-1)ELISA试剂盒现货】

QY-17512 Anti-PR（Progestogerone receptor）孕激素受体抗体                                                      

QY-17522 Anti-PRL (Prolactin)鼠抗人泌乳素抗体                                               

QY-17532 Anti-Prolylhydroxylase脯氨酸羟化酶抗体                                                  

QY-17542 Anti-PS-1 （Presennillin-1/S182）（NT）早老素蛋白-1抗体                                                      

QY-17552 Anti-PS-1（Presennillin-1/S182）（CT）早老素蛋白-1抗体                                               

QY-17562 Anti-PS-1（Presennillin-1/S182）（NT）早老素蛋白-1抗体                                               

QY-17572 Anti-PSA (anti-Prostate Specific Antigen (PSA) , for conjugation ａnd detection)鼠抗人前列腺特异性抗原抗体                                                       

QY-17582 Anti-PSCA (Prostate Stem Cell Antigen)抗前列腺干细胞抗原抗体                                                     

QY-17592 Anti-PSD-95突触后密度蛋白95抗体                                                

QY-17602 Anti-P-selectinP选择素抗体                                                

QY-17612 Anti-PTEN/MMAC1一种肿瘤抑制基因                                                       

QY-17622 Anti-PTHrP (Parathyroid Hormone- related Protein)甲状旁腺激素相关蛋白抗体                                                     

QY-17632 Anti-PTP-1B/pTyr (Protein-tyrosine phosphatase, non-receptor :p-tyr)蛋白酪氨酸磷酸酶-1B/磷酸化酪氨酸抗体                                                 

QY-17642 anti-Parvalbumin微白蛋白/细小清蛋白抗体                                                     

QY-17652 Anti-Rad51Rad51抗体                                                 

QY-17662 Anti-RAR-α (Retinoic acid -R-α)维甲酸受体-α 抗体                                                       【大鼠血管内皮细胞生长因子受体2(VEGFR-2/Flk-1)ELISA试剂盒现货】

QY-17672 Anti-RAR-β(Retinoic acid -R-β)维甲酸受体-β抗体                                                 

QY-17682 Anti-RASSF1A（Ras association domain family 1A）Ras相关区域家族1A抗体                                               

QY-17692 Anti-Rat IgA兔抗大鼠sIgA抗体                                                 

QY-17702 Anti-Resistin抵抗素抗体                                                      

QY-17712 Anti-RhoA (RhoA protein)RhoA抗体                                                     

QY-17722 Anti-ROCK1 (Rho-associated coiled-coil containing protein kinase 1)Rho相关蛋白激酶1抗体                                                

QY-17732 Anti-ROCK2 (Rho-associated coiled-coil containing protein kinase 2)Rho相关蛋白激酶2抗体                                                

QY-17742 Anti-RRAD (Ras-related associated with diabetes )RRAD抗体