价格详细资料：试剂盒价格电议，齐一生物销售：021－6034 8496；181214 53965；173021 04490,齐一生物科技（上海）有限公司提供ELISA试剂盒受到了广大研究所.中科院科研单位一致肯定和认同。齐一生物大品牌保证，倾力为国内外科研院校实验室提供*优质产品。

【人游离三碘甲状腺原氨酸(Free-T3)ELISA试剂盒】注意事项

1. 当混合蛋白溶液时应尽量轻缓，避免起泡。

2. 洗涤过程非常重要，不充分的洗涤易造成假阳性。

3. 一次加样时间控制在5分钟内，如标本数量多，推荐使用排枪加样。

4. 请每次测定的同时做标准曲线，做复孔。

5. 如标本中待测物质含量过高，请先稀释后再测定，计算时请*后乘以稀释倍数。

6. 在配制标准品、检测溶液工作液时，请以相应的稀释液配制，不能混淆。

7. 底物请避光保存。

8. 不要用其它生产厂家的试剂替换试剂盒中的试剂。

FOR RESEARCH USE ONLY

Drug Names

Generic Name：

This kit can be used for determination of serum, plasma ａnd liquid samples Organization Content.

The experimental principle:

The product levels were measured in samples of the kit by double antibody sandwichmethod. The product with the purified antibody coated microtiter plate, made of solid phase antibody, to package is the product antigen monoclonal antibodies are then added to the micropores, the product and then with HRP labeled antibody binding, the formation of antibody - antigen - antibody complex enzyme label, after thorough washing with TMB chromogenic substrate. TMB in the HRP enzyme catalytic conversion into the blue, and in the action of acid into the final yellow. This product is positively related to the depth of color and in the samples. Instrument measured absorbance in the 450nm wavelength with ELISA (OD), the product concentration in the samples was calculated by standard curve.

Materials provided with the kit

Materials provided with the kit 96 determinations Storage

User manual   1  

Closure plate membrane   2  

Sealed bags   1  

Microelisa stripplate    1   2-8℃

Standard：360ng/L 0.5ml×1 bottle   2-8℃

Standard diluent  1.5ml×1 bottle   2-8℃

HRP-Conjugate reagent    6ml×1 bottle 2-8℃

Sample diluent    6ml×1 bottle 2-8℃

Chromogen Solution A 6ml×1 bottle 2-8℃

Chromogen Solution B 6ml×1 bottle 2-8℃

Stop Solution 6ml×1 bottle 2-8℃

wash  solution    （20ml×30 fold）

×1bottle  2-8℃

Specimen requirements

1.    serum- coagulation at room temperature 10-20 mins，centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. 【人游离三碘甲状腺原氨酸(Free-T3)ELISA试剂盒】

2.    plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

3.    Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax ａnd cerebrospinal fluid Reference to it.

4.    cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS（PH7.2-7.4）, Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells ａnd release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

5.    Tissue samples- After cutting samples, check the weight,add PBS（PH7.2-7.4）, Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS（PH7.4）, Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant.

6.    extract as soon as possible after Specimen collection,and according to the relevant literature, ａnd should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.

7.    Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.

Assay procedure

1.Dilute ａnd add sample to Standard: set 10 Standard wells on the ELISA plates coated, add Standard 100μl to the first ａnd the second well, then add Standard dilution 50μl to the first ａnd the second well, mix; take out 100μl form the first ａnd the second well then add it to the third ａnd the forth well separately. then add Standard dilution 50μl to the third ａnd the forth well ,mix ; then take out 50μl from the third ａnd the forth well discard, add 50μl to the fifth ａnd the sixth well ,then add Standard dilution 50μl to the fifth ａnd the sixth well, mix ; take out 50μl from the fifth ａnd the sixth well ａnd add to the seventh ａnd the eighth well, then add Standard dilution 50μl to the seventh ａnd the eighth well ,mix ; take out 50μl from the seventh ａnd the eighth well ａnd add to the ninth ａnd the tenth well, add Standard dilution 50μl to the ninth ａnd the tenth well, mix , take out 50μl from the ninth ａnd the tenth well discard(add Sample 50μl to each well after Diluting ,(density: 240ng/L,160ng/L ,80ng/L,40ng/L, 20ng/L)

2.add sample：Set blank wells separately (blank comparison wells .don’t add sample ａnd HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, ａnd Gently mix. 【人游离三碘甲状腺原氨酸(Free-T3)ELISA试剂盒】

3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.

4.Configurate liquid: 30-fold (or 20-fold)wash solution diluted 30-fold (or 20-fold) with distilled water ａnd reserve.

5.washing：Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.

6.add enzyme：Add HRP-Conjugate reagent 50μl to each well, except  blank well.

7.incubate：Operation with 3.

8.washing：Operation with 5.

9.color：Add Chromogen Solution A 50ul ａnd Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃

10.Stop the reaction：Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).

11.assay：take blank well as zero , Read absorbance at 450nm after Adding Stop Solution ａnd within 15min.

Important notes

1.    The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.

2.    washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.

3.    add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 mins, if the number of sample is much , recommend to use Volley . 【人游离三碘甲状腺原氨酸(Free-T3)ELISA试剂盒】

4.    if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente ａnd multiplied by the dilution factor.（×n×5）.

5.    Closure plate membrane only limits the disposable use, to avoid cross-contamination.

6.    The substrate evade the light preservation.

7.    Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.

8.    All samples, washing buffer ａnd each kind of reject should according to infective material process.

9.    Do not mix reagents with those from other lots.

Calculate：

Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density ａnd the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.

Storage ａnd validity

1．Storage：  2-8℃.

2．validity： six months.

QY-HC0117       人肺癌细胞 Calu-1         Calu-1                                           

QY-HC0118       人肾透明细胞癌皮肤转移细胞 Caki-1          Caki-1                                            

QY-HC0119       人胰腺癌细胞 SW 1990         SW1990                                        

QY-HC0120       人胰腺癌细胞 CFPAC-1 CFPAC-1                                        

QY-HC0121       人非雄激素依赖型前列腺癌细胞 Tsu-Pr1   Tsu-Pr1                                         

QY-HC0122       人急性淋巴母细胞白血病细胞 MT-4  MT-4                                             

QY-HC0123       人卵巢癌细胞 NIH/OVCAR-3         NIH/OVCAR-3                                      

QY-HC0125       人喉癌细胞 TU 686        TU686                                           

QY-HC0126       人喉癌细胞 TU 212        TU212                                           

QY-HC0127       人喉癌细胞 M2e M2e                                     

QY-HC0129       "人甲状腺鳞癌细胞 SW579[SW 579; SW-

579]"        "SW579[SW579;SW-

579]"                                             

QY-HC0130       人结肠腺癌细胞 LS 180         LS180                                             【人游离三碘甲状腺原氨酸(Free-T3)ELISA试剂盒】

QY-HC0131       人宫颈癌细胞 C-33AC-33 A           C-33AC-33A                                          

QY-HC0132       人宫颈癌肠转移细胞 CaskiCa Ski CaskiCaSki                                             

QY-HC0133       人胰腺癌细胞 Patu 8988       Patu8988                                     

QY-HC0134       人羊膜细胞 WISH WISH                                             

QY-HC0135       人鼻咽癌细胞 HNE2      HNE2                                            

QY-HC0136       人十二脂肠腺癌细胞 HuTu-80     HuTu-80                                       

QY-HC0137       人子宫内膜腺癌细胞 RL95-2       RL95-2                                          

QY-HC0138       人胰腺癌细胞 PANC-1           PANC-1                                         

QY-HC0139       人肺癌细胞 PC-9PC9      PC-9PC9                                        

QY-HC0140       人转移胰腺癌细胞 AsPC-1    AsPC-1                                          

QY-HC0141       人急性非B非T淋巴细胞白血病细胞 Reh         Reh                                      

QY-HC0142       人肺癌细胞 MSTO-211H        MSTO-211H                                          

QY-HC0143       人肺成纤维样细胞 HLF         HLF                                       

QY-HC0144       人B淋巴瘤细胞 Farage        Farage                                          

QY-HC0146       人肺癌细胞 NCI-H596   NCI-H596                                     

QY-HC0147       人肺鳞癌细胞 SK-MES-1        SK-MES-1                                      【人游离三碘甲状腺原氨酸(Free-T3)ELISA试剂盒】

QY-HC0148       人结肠癌细胞 RKO         RKO                                      

QY-HC0149       人鼻咽癌细胞 SUNE-1亚株-6-10B        SUNE-1亚株-6-10B                                     

QY-HC0150       人胚肾细胞 293A  293A                                             

9019565  Clamp, cable 2 wires 2 tabs, Cxx               CNY                             

9019543  Bow spring, table rail, Cxx                 CNY                             

9019510  CHAMBER MAIN VAC STATION 1820                 CNY                             

9019505  PLATE SEPARATOR 48 WELL HIGH            CNY                             

204256     QuantiFast Probe PCR Kit (2000)               CNY                             

204174     Rotor-Gene SYBR Green RT-PCR Kit (400)       The Rotor-Gene SYBR Green RT-PCR Kit is dedicated for use with the Rotor-Gene Q cycler, providing ultrafast, highly specific quantification in real-time one-step RT-PCR using SYBR Green I.   CNY                             

204154     QuantiFast SYBR Green RT-PCR Kit (400)                  CNY                             

9019424  Wiring, lower heater, RG3                CNY                             

9019417  Fan, lid, RG3              CNY                             

9019393  Solenoid, cooling with flap, RG3                CNY                             

9019386  Photo Multiplier Tube with cable, RG3             CNY                             

9019368  Damper, lid, RG6               CNY                              【人游离三碘甲状腺原氨酸(Free-T3)ELISA试剂盒】

9019347  Motor, 12VDC Heater fan, RG6                CNY                             

9234697  Manual Hardware Plato 7                 CNY                             

9020477  Gasket, front plate, GSC           CNY                             

9234463  Coupling, CPC, inline, female, 8mm                   CNY                             

1027285  AllStars Neg. siRNA AF 546 (5 nmol)                  CNY                             

1022564  Mm/Hs_MAPK1 control siRNA (5 nmol)           CNY                             

9019205  Opto - Stacker, PyroQ96MD              CNY                             

9019204  Opto - Robot Z motor , PyroQ96MD                  CNY                             

9019200  Hinge kit - Top cover, PyroQ96MD            CNY                             

1022073  Luciferase GL3 siRNA (5 nm)             CNY                             

1022064  GFP-22 siRNA (5 nm)                  CNY                             

1018996  PCR Buffer, 10 x (22ml/30),KG           CNY                             

1064605  MagAttract M48 DNA Manual Kit  (200)                CNY                             

1066851  PG FMDV RT-PCR Assay (48) LE Z              CNY                             

1061054  HBV PCR REAGENT I                  CNY                             

1061069  UU PCR REAGENT II                   CNY                              【人游离三碘甲状腺原氨酸(Free-T3)ELISA试剂盒】

1061079  NG PCR REAGENT II                   CNY                            

9019886  Lid, label 4CH, RG3           CNY                             

9019875  Cover, main, silver, RGQ           CNY                             

9019836  IPC Hard Drive PCA6751,  PyroQ96ID             CNY                             

9019769  Valve, shutoff, V2, QX                CNY