【友情提示】：本产品仅供科研研究使用，不得用于人体临床直接检测。避免给您带来不必要的损失，请仔细阅读购买说明！

本试剂盒仅供研究使用，齐一生物销售：021－6034 8496；181214 53965；173021 04490欢迎选购！

操作步骤

1.准备试剂，样品和标准品

2.加入准备好的样品和标准品，37℃反应30分钟

3.洗板5次，加入酶标试剂，37℃反应30分钟

4.洗板5次，加入显色液A、B，37℃显色15分钟

5.加入终止液

6.15分钟之内读OD值

7.计算
齐一生物供应ELISA试剂盒检测的目的是为实验提供准确可靠的定量分析实验依据。为了保证实验数据的可靠性，在实验过程中必须坚持全面质量控制和全过程质量控制。在收集标本前都必须有一个完整的计划。

【人凋亡信号调节激酶I(ASK-1)ELISA试剂盒厂家】样本保存条件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6个月

3.-50℃以下的冰箱，可以放一年

【人凋亡信号调节激酶I(ASK-1)ELISA试剂盒厂家】样本收集

1.血清样本用干燥管（红色）或者促凝管（黄色）收集

2.血浆用抗凝管，一般有EDTA抗凝（紫色）、枸橼酸钠抗凝（黑色或者蓝色）

3.动物组织匀浆（细胞）：

              1g组织加入9ml PBS（浓度为0.1M，pH为7.4的）测分泌型指标

              1g组织加入1ml组织裂解液加入8ml PBS 测细胞内

粪便样本：1g样本+9ml PBS（浓度为0.01M，pH为7.4的）

没有PBS的话也可以用一下生理盐水，还是用PBS

【人凋亡信号调节激酶I(ASK-1)ELISA试剂盒厂家】样本离心转速及时间

1.试管采集的样本：3000转，离心五分钟取上清

2.离心管或者EP管采集的样本：5000－－8000转3分钟取上清

3..样本保存－－如果样品不立即使用，应将其分成小部分-70 ℃保存，避免反复冷冻。尽可能的不要使用溶血或高血脂血。如果血清中大量颗粒，检测前先离心或过滤。不要在37℃或更高的温度加热解冻。应在室温下解冻并确保样品均匀地充分解冻。

【人凋亡信号调节激酶I(ASK-1)ELISA试剂盒厂家】以上样本采集及处理方式供您参考，如有问题请来电，齐一生物销售：021－6034 8496；181214 53965；173021 04490

QKG-312              规格：500ml                                      

QKG-313       丽春红染色试剂 Ponceau Stain Reagent     规格：100ml                             

QKG-314              规格：500ml                                      

QKG-315       PBS (pH7.5, 10×) 规格：500ml                                      

QKG-316       PBST (pH7.5, 10×)      规格：500ml                                      

QKG-317       TBS (pH8.0, 10×) 规格：500ml                                      

QKG-318       TBST (pH8.0, 10×)      规格：500ml                                       【人凋亡信号调节激酶I(ASK-1)ELISA试剂盒厂家】

QKG-319       Tris-Glycine Transfer Buffer (pH8.3, 10×) 规格：500ml                                      

QKG-320       Western Blot Ladder Pen       规格：1000次                                 

QKG-321              规格：5000次                                     

QKG-322       NC膜 (0.45 μm) 规格：14cm×20cm/张                                     

QKG-323       Annexin V-FITC/PI 凋亡检测试剂盒 规格：50次                             

QKG-324       细胞周期与凋亡检测试剂盒       规格：50次                             

QKG-325       Annexin V-Alexa Fluor 488/PI 凋亡检测试剂盒 规格：50次                             

QKG-326       Annexin V-Alexa Fluor 647/PI 凋亡检测试剂盒 规格：50次                             

QKG-327       Annexin V-PE/7-AAD 凋亡检测试剂盒      规格：50次                             

QY1101   Annexin V-FITC/PI细胞凋亡检测试剂盒   20                               

QY1102   Annexin V-EGFP/PI细胞凋亡检测试剂盒  10                               

QY-AB1103     线粒体膜电位检测试剂盒(JC-1) 10                               

QY-AB1104     DNA Ladder检测试剂盒      20                               

QY-AB1105     TUNEL细胞凋亡原位检测试剂盒（显色法，通用型）  20                               

QY-AB1106     TUNEL细胞凋亡原位检测试剂盒（显色法，石蜡切片）      20                               

QY-AB1107     TUNEL细胞凋亡原位检测试剂盒（显色法，细胞）      20                                【人凋亡信号调节激酶I(ASK-1)ELISA试剂盒厂家】

QY-AB1108     TUNEL细胞凋亡原位检测试剂盒（显色法，冰冻切片）      20                               

9QY-AB1109   一步法TUNEL细胞凋亡原位检测试剂盒（通用型）     20                               

QY-AB1110     TUNEL细胞凋亡原位检测试剂盒（荧光及显色法，石蜡切片）  20                               

QY-AB1111     TUNEL细胞凋亡原位检测试剂盒（荧光及显色法，细胞）  20                               

QY-AB1112     TUNEL细胞凋亡原位检测试剂盒（荧光及显色法，冰冻切片）  20                               

QY-AB1113     Caspase-3活性检测试剂盒(分光光度法)   20                               

QY-AB1114     Caspase-8活性检测试剂盒(分光光度法)   20                               

QY-AB1115     Caspase-9活性检测试剂盒(分光光度法)   20                               

QY-AB1116     细胞周期检测试剂盒    20                               

QY-AB1117     MTT细胞增殖及细胞毒性检测试剂盒      500                             

133156    NeXtalStock BIS-TRIS Propane pH8.5 (100)             CNY                           

133151    NeXtalStock BIS-TRIS pH 6.5 (100)           CNY                           

133074    NeXtalStock PEG 1,000 (200)             CNY                           

133071    NeXtalStock Nickel(II) chloride (200)         CNY                           

870111    therascreen EGFR RGQ PCR Kit CE (24)           CNY                           

805923    REPLI-g Service,  Single Tube (100 µg)           CNY                           

9015356  Interlock switch 2, for maintenance            CNY                           

9015345  Board, PSB            CNY                           

133042    NeXtalStock DL-Malic Acid (200)        CNY                           

133037    NeXtalStock Dimethyl sulfoxide (200)         CNY                           

9018394  Key, User, red, QIAxcel FA          CNY                           

9018337  Tubing, Valve to injection needle (set)          CNY                           

9001669  Rotor-Gene Q 6plex (w/o PC) PrioPLUS     "Rotor-Gene Instrument

Installation of Rotor-Gene & Introductory training

Warranty extension to 3 yrs

Loaner available in 2 days*

Annual Inspections (1 onsite PM per yearr)

Priority service provision over non-contract customers

Documented installation & PM report

Unlimited number of repair service

All cost covered (travel, labor, parts)"   CNY                           

9001668  Rotor-Gene Q 6plex (w/o PC) Priority  Rotor-Gene Q 6plex (w/o PC) Priority includes the Rotor-Gene Q system, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with on site introductory training. Priority package extends warranty period of the system to 2 years with shipment to Repair Center Service has response turnaround time (repair) 7-10 days. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Replacement system (Loaner) provision included (provision time 2-3 days) with transportation cost coverage. The service package includes all parts, labor ａnd travel costs with installation performance ａnd service visits documented on a GMP/GLP-compliant Field Service documents. CNY                           

951336    MagAttract DNA Blood Mini M48 Kit (192)  MagAttract Suspension B, buffers, ａnd RNase-free water for up to 192 x 200 ul preps   CNY                           

37582     Qproteome Nuclear Protein Kit            CNY                            【人凋亡信号调节激酶I(ASK-1)ELISA试剂盒厂家】

9001640  Rotor-Gene Q 5plex System         CNY                           

290013    mericon Salmonella spp Kit (24)           CNY                           

231124    QIAGEN PCR Cloning Kit (40)     For 40 reactions: 2x Ligation Master Mix (200 ul), pDrive Cloning Vector (2.0 ug), distilled water (1.7 ml)   CNY                           

133149    NeXtalStock BICINE pH 9.0 (200)              CNY                           

219610    miRNeasy Serum/Plasma Spike-In Control          CNY                           

9015319  Sensor, photo, EE-SX673A          CNY                           

133014    NeXtalStock Ammonium phosphate (200)           CNY                           

1045800  Ni-NTA Superflow Cartridge Kit (1ml)         CNY                           

950107    Reagent Pack, DX  The Reagent Pack, DX,  which is used together with the DNA Plasticware Pack, QIAxtractor (#950037) provides fully automated DNA purification on the QIAxtractor. The combination of reagents ａnd plasticware enables purification of genomic DNA from virtually all types of sample, with a throughput of 8-96 samples per run.  CNY                           

9018319  Fuses 8A, centrifuge board (110V), QIAC           CNY                           

9018305  EZ1 Advanced DSP DNA Blood Card           CNY                           

9015285  Filter, HEPA, M48         CNY                           

9018301  EZ1 Advanced DNA Bacteria Card        CNY                           

9018289  Belttool, Y-Drive, Adjust        CNY                           

800388    Manual Sequence Editing              CNY